Gxininisa kwisakhono sokufunda iintlobo ngeentlobo zeentlobo zophando, umzekelo ngemeko engundoqo evela kubathengi be-BMKGENE.Kutshanje ipapashwe kuhlelo lwalo nyaka lwe-Plant Biotechnology Journal, inqaku elinesihloko esithi "i-Chromosome-level Wild Hevea brasiliensis Genome: Ukuxhobisa i-Genomic-Assisted Breeding kunye ne-Unearthing Vital Loci kwi-Elevated Rubber Yield" imele ireferensi ebalulekileyo.
Umthi werabha waseBrazil (Hevea brasiliensis) ume mde njengomthombo obalulekileyo werabha yendalo.Ukutyhubela inkulungwane edlulileyo, iindlela zokuzalanisa ngokwesintu zibangele ukwanda okumangalisayo okuphindwe kathandathu kwimveliso yerabha.Nangona kunjalo, isiseko sofuzo esisisiseko esinoxanduva lokomeleza isivuno serabha esinokubakho sihleli singumnqa.
Olu phononongo luqale ekwakhiweni kwegenome yomgangatho wechromosome yomthi werabha yasendle, kusetyenziswa i-synergy yeNanopore yolandelelwano lokufunda ixesha elide, ukulandelelana kwe-Illumina NGS, kunye nobuchwepheshe be-Hi-C.
Ngokunxuseneyo, i-trove yemithombo ye-germplasm ye-147, equka ukuhluka kwesivuno esiphezulu, iintlobo zasendle, kunye neentlobo ezinxulumene nazo, zahlanganiswa ukuze ziphelele i-genome resequencing (WGS) kusetyenziswa iqonga le-Illumina.Ulandelelwano lwedatha luye lwahlahlelwa ngokungqongqo kwimfuza yabemi, okwenza ukuba ukwahlulwa kobume babemi, iyantlukwano, kunye nokungalungelelani konxibelelwano.Olu hlalutyo lunika ukukhanya kwiiyantlukwano zasekhaya kuluntu lonke.
Uphando olungaphaya lubandakanya uhlalutyo olubandakanya i-Fst, π, Tajima's D, kunye ne-LD, ejolise ekutyhileni imiqondiso yokukhetha evela kwimveliso ephuculweyo yelatex, egxile kakhulu kwiiclones zeWickham.
Ukuphelela kuHlahlelo lwe-Genome-wide Association Analysis (GWAS), abamakishi abadityaniswe neempawu zesivuno kunye nofuzo olunxulumene nesivuno lwachongwa.Uqinisekiso lokuqala luveze imisebenzi ekhutshelweyo eyahlukileyo kuzo zonke iinguqulelo ezahlukeneyo zemfuza ezimbini zeERF, ibonelela ngomboniso wokuqala wokubaluleka kokusebenza kwazo.Uqinisekiso olongezelelweyo lucwangciswe kuphando oluzayo.
I-BMKGENE iyazingca ngokuba negalelo kulandelelwano olubanzi lwegenome yolu luntu lukhulu, kunye nendima enkulu kuhlalutyo lwe-bioinformatics.Amava ethu amakhulu aquka ulandelelwano lwe-NGS/TGS kunye nohlalutyo lwe-multiomics bioinformatics kulo lonke uluhlu lweentlobo ezili-1000+.Sijonge phambili kwithemba lokusebenzisana nawe kwiiprojekthi zakho ezizayo.
Cofa apha ukuzefunda ngakumbi malunga nesi sifundo
Ixesha lokuposa: Aug-29-2023