I-BMKGENE inebhongo ngokubhengeza ukufumaneka kwePacBio yolandelelwano oluphambili, iRevio, esinceda ukuba sinikezele ngeziphumo eziphezulu kunye neenkonzo zolandelelwano ezifundwe ixesha elide kubaphandi.
*Kutheni ukhetha iPacBio Revio?
*Izicelo zePacBio Revio
•Ulandelelwano lweGenome
1.De Novoindibano yegenome- I-Revio inegalelo ekulandeleniseni umgangatho ophezulu kunye nokudityaniswa kweegenomes ezinkulu kakhulu nezintsokothileyo, ibonelela ngengqiqo ebalulekileyo kuguquko lwemfuza, ukwakheka kwemfuza, kunye nomsebenzi.
2.T2T genome- I-Revio, idibaniswe neendlela ezifana ne-Hi-C, inokuphucula umgangatho wee-genomes eziqhelekileyo kwaye incede iintlobo ezininzi zifumana i-T2T-level reference genome, eyenza abaphandi baqonde ngcono ukuhlukahluka kofuzo ngaphakathi naphakathi kwabantu.
3.Ulandelelwano lwegenome yonke- I-Revio inokubonelela ngengqiqo ebanzi kwisakhiwo kunye nomsebenzi we-genomes yonke, kuquka i-SNP, i-Indel, i-SV, i-CNV, njl., ngokukodwa kwi-genetics enkulu.
4.Ulandelelwano lwe-Pan-genome- I-Revio ilungele ukulandelelanisa ngokufanelekileyo nangokungabizi kakhulu kunye nokudibanisa iigenomes ezininzi zeentlobo ngaxeshanye ukwakha i-pan-genome, ukubonelela ngengqiqo ebalulekileyo kwinguqu ye-genomic kunye ne-evolution.
•Ulandelelwano lwe-Transcriptome
1.Ulandelelwano lwemibhalo ekhutshelweyo yobude obupheleleyo-Ngobude bayo obude bokufunda, iRevio yenza ukuba ulandelelwano lombhalo olupheleleyo olukumgangatho ophezulu, lubonelele ngombono ophangaleleyo kwezinye iindlela zokwahlulahlula, ukudityaniswa kofuzo, kunye nokubonakaliswa kwe-isoform.
I-2.Iprofayili yokubonisa ubude obupheleleyo beesampuli ezinkulu-I-Revio inokubonelela ngobungakanani obuchanekileyo obuphezulu bamanqanaba embonakalo yemfuza kuzo zonke iisampulu ezinkulu, ivumela ukuqonda okubalulekileyo kulawulo lwemfuza kunye nokwahlulwa kweeseli.
3.Ulandelelwano lwe-transcriptome yeseli enye- I-Revio inokubonelela nge-high-resolution yedatha ye-transcriptomics yeseli enye, eyenza abaphandi bafunde i-heterogeneity yeselula kunye nokuchonga iintlobo ezinqabileyo zeeseli.
•Ukulandelelana kweMicrobial
1.16S ulandelelwano olupheleleyo-I-Revio yenza umgangatho ophezulu we-16S rRNA ukulandelelana, ukubonelela ngeenkcukacha ezicacileyo malunga nokubunjwa koluntu lwe-microbial kunye nomsebenzi.
2.Ulandelelwano lweamplicon olufunda ixesha elide-I-Revio ifaneleke ngokulandelelana kwe-amplicon efundwa ixesha elide, eyenza abaphandi bafunde ukuhlukahluka kwe-microbial kwii-ecosystems ezinzima.