I-Evolutionary Genetics
Iinkonzo eziluncedo
Takagi et al.,Ijenali yezityalo, 2013
● Uqikelelo lokwahlukana kweentlobo zexesha kunye nesantya esisekwe kwiiyantlukwano kwinqanaba le-nucleotide kunye ne-amino acid.
● Ukutyhilwa kobudlelwane obuthembeke ngakumbi bephylogenetic phakathi kwezidalwa ezinempembelelo encitshisiweyo yendaleko eguquguqukayo kunye nendaleko enxuseneyo.
● Ukwenza amakhonkco phakathi kweenguqu zemfuza kunye neephenotypes ukuze kufunyanwe imizila yemfuza enxulumene neempawu
● Ukuqikelela ukwahlukana kwemizila yemfuza, ebonisa ukuba izinto eziphilayo zazivelela
● Ixesha lokuguqula ngokukhawuleza
● Amava abanzi: I-BMK iqokelele amava amakhulu kuluntu kunye neeprojekthi ezinxulumene ne-evolution iminyaka engaphezu kwe-12, equka amakhulu eentlobo zezilwanyana, njl.njl. kwaye ibe negalelo kwiiprojekthi ezingaphezulu kwama-80 ezikumgangatho ophezulu ezipapashwe kwi-Nature Communications, i-Molecular Plants, i-Plant Biotechnology Journal, njl.
IiNgcaciso zeNkonzo
Izixhobo:
Ngokwesiqhelo, ubuncinci abathathu abancinci (umzekelo, iintlobo ezingaphantsi okanye iintlobo) bayacetyiswa.Icandelwana ngalinye kufuneka liqulathe abantu abangekho ngaphantsi kwe-10 (Izityalo ＞15, zinokuncitshiswa kwiintlobo ezinqabileyo).
Iqhinga lokulandelelanisa:
* I-WGS inokuqeshwa kwiintlobo ezinomgangatho ophezulu we-genome, ngelixa i-SLAF-Seq isetyenziswa kwiintlobo okanye ngaphandle kwe-genome ye-reference, okanye i-genome ye-reference yomgangatho ophantsi.
| Isebenza kubungakanani begenome | WGS | I-SLAF-Tags (×10,000) |
| ≤ 500Mb | 10×/ngabanye | I-WGS iyacetyiswa ngakumbi |
| 500 Mb - 1 Gb | 10 | |
| I-1 Gb - 2 Gb | 20 | |
| ≥2 Gb | 30 |
Uhlalutyo lwe-Bioinformatics
● Uhlalutyo lwendaleko
● Ukutshayela okukhethiweyo
● Ukuhamba kwemfuza
● Imbali yamanani abemi
● Ixesha lokuhlukana
IiMfuno zeSampulu kunye nokuhanjiswa
IiMfuno zeSampulu:
| Iintlobo | Izicubu | WGS-NGS | ISLAF |
| Isilwanyana
| Izicubu ze-Visceral |
0.5~1g
|
0.5g
|
| Izicubu zezihlunu | |||
| Igazi leMammalian | 1.5mL
| 1.5mL
| |
| Inkukhu / Igazi lentlanzi | |||
| Isityalo
| Igqabi Elitsha | 1~2g | 0.5~1g |
| Ipetali/Isiqu | |||
| Ingcambu/Imbewu | |||
| Iiseli | Iseli Cultured |
| gDNA | Ukugxininisa | Isixa (ug) | OD260/OD280 |
| ISLAF | ≥35 | ≥1.6 | 1.6-2.5 |
| WGS-NGS | ≥1 | ≥0.1 | - |
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
*Iziphumo zedemo eziboniswe apha zonke zivela kwiigenomes ezipapashwe nge-BMKGENE
I-1.Uhlalutyo lwe-Evolution luqulethe ukwakhiwa komthi we-phylogenetic, isakhiwo sabantu kunye ne-PCA esekelwe kwiinguqu zofuzo.
Umthi we-Phylogenetic umele ubudlelwane be-taxonomic kunye ne-evolutionary phakathi kweentlobo kunye nezinyanya eziqhelekileyo.
I-PCA ijolise ekuboneni ukusondelana phakathi kwabantu abancinci.
Ubume benani labantu bubonisa ubukho benani labemi abahlukileyo ngokwemfuza ngokwemiqukumbelo yeallele.
Chen, kunye.al.,PNAS, 2020
2.Ukutshayela okukhethiweyo
Ukutshayela okukhethiweyo kubhekiselele kwinkqubo apho isiza esiluncedo sikhethwayo kwaye ukuphindaphindwa kweendawo ezingathathi hlangothi zinyuswa kwaye ezo sayithi zingadityaniswanga ziyancipha, okukhokelela ekuncipheni kommandla.
Ubhaqo olubanzi lwe-Genome kwimimandla ekhethiweyo yokutshayela kusetyenzwa ngokubala isalathisi semfuza yabemi（π，Fst, Tajima's D) yazo zonke ii-SNP ngaphakathi kwefestile etyibilikayo (100 Kb) kwinqanaba elithile (10 KB).
Ukwahluka kwe-Nucleotide (π)
UTajima D
Ukulungiswa kwesalathiso(Fst)
Wu, kunye.al.,Isityalo seemolekyuli, 2018
3.Gene Flow
Wu, kunye.al.,Isityalo seemolekyuli, 2018
Imbali ye-4.Demographic
Zhang, kunye.al.,I-Ecology yendalo kunye ne-Evolution, 2021
5.Ixesha leDivergence
Zhang, kunye.al.,I-Ecology yendalo kunye ne-Evolution, 2021
Ityala le-BMK
Imephu yokwahluka kwegenomic ibonelela ngokuqonda kwisiseko sofuzo seSpring Chinese Cabbage(Brassica rapa ssp. Pekinensis) ukhetho
Ipapashiwe: Isityalo seemolekyuli, 2018
Iqhinga lokulandelelanisa:
Ukulandelelana: ubunzulu bolandelelwano: 10×
Iziphumo eziphambili
Kolu phononongo, iikhaphetshu ezingama-194 zaseTshayina zacutshungulwa ukuze ziphinde zilandelelane ngobunzulu obuphakathi kwe-10 ×, eyavelisa i-1,208,499 SNPs kunye ne-416,070 InDels.Uhlalutyo lwe-Phylogenetic kule migca ye-194 ibonise ukuba le migca ingahlulwa ibe yi-ecotypes ezintathu, intwasahlobo, ihlobo kunye nekwindla.Ukongeza, ubume boluntu kunye nohlalutyo lwe-PCA lubonise ukuba iklabishi yaseTshayina yasentwasahlobo yavela kwikhaphetshu yasekwindla eShandong, eChina.Ezi ke emva koko zaziswa eKorea nase Japan, zawela nemigca yalapha ekhaya kwaye ezinye iindidi zokubolitha kade zaziswa emva eTshayina kwaye ekugqibeleni zaba yikhaphetshu yase-Spring Chinese.
Ukuskena okubanzi kweGenome kwintlakohlaza yekhaphetshu zaseTshayina kunye nekhaphetshu zasekwindla ekukhethweni kutyhilwe iiloci ezingama-23 zegenomic eziye zadlula kukhetho olomeleleyo, ezimbini kuzo zadityaniswa nommandla wolawulo lwebholiti esekwe kwimephu yeQTL.Le mimandla mibini ifunyenwe iqulethe imfuza engundoqo elawula ukudubula kweentyatyambo, i-BrVIN3.1 kunye ne-BrFLC1.Ezi zofuzo zimbini ziphinde zaqinisekiswa ukuba zibandakanyeke kwixesha lokuvalwa ngophononongo lwe-transcriptome kunye novavanyo lwe-transgenic.
 Uhlalutyo lwesakhiwo sabemi kwiikhaphetshu zaseTshayina |  Ulwazi lwemfuzo ekukhethweni kwekhaphetshu yaseTshayina |
Tongbing, et al."Imephu yoLwahluko lweGenomic ibonelela ngeMiyalelo kwiSiseko seMfuzo yeNtwasahlobo yeKhaphetshu yaseTshayina (Brassica rapa ssp.pekinensis)Ukhetho."Izityalo zeMolekyuli,11 (2018): 1360-1376.
