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I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)
I-ChIP-Seq inikezela ngeprofayili ye-genome-wide-wide ye-DNA ekujoliswe kuyo yokuguqulwa kwe-histone, izinto ezibhaliweyo, kunye nezinye iiprotheni ezinxulumene ne-DNA.Idibanisa ukukhethwa kwe-chromatin immuno-precipitation (i-ChIP) ukubuyisela i-protein-DNA complexes ethile, kunye namandla okulandelelanisa isizukulwana esilandelayo (NGS) ukulandelelana kwe-high-throughput ye-DNA efunyenweyo.Ukongeza, ngenxa yokuba iiprotheyini ze-DNA zifunyenwe kwiiseli eziphilayo, iindawo zokubopha zinokuthelekiswa kwiintlobo zeeseli ezahlukeneyo kunye nezicubu, okanye phantsi kweemeko ezahlukeneyo.Izicelo zisusela kulawulo lokubhaliweyo ukuya kwiindlela zophuhliso kwiindlela zokugula nangaphaya.
Iqonga: Illumina NovaSeq 6000
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Ulandelelwano lwegenome bisulfite epheleleyo
I-DNA methylation kwindawo yesihlanu kwi-cytosine (5-mC) inempembelelo ebalulekileyo kwi-gene expression kunye nomsebenzi weselula.Iipateni ze-methylation ezingaqhelekanga ziye zadibaniswa neemeko ezininzi kunye nezifo, ezifana nomhlaza.I-WGBS ibe ngumgangatho wegolide wokufunda i-genome-wide methylation kwisisombululo sesiseko esinye.
Iqonga: Illumina NovaSeq6000
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I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye nokuSetyenziswa kwe-High throughput (ATAC-seq)
I-ATAC-seq yindlela ephezulu yokulandelelanisa i-genome-wide-wide chromatin accessibility, ebaluleke kakhulu kulawulo lwe-epigenetic lwehlabathi jikelele lwe-gene expression.Iiadaptha zolandelelwano zifakwe kwimimandla yechromatin evulekileyo nge-hyperactive Tn5 transposase.Emva kokwandiswa kwePCR, ithala leencwadi lolandelelwano liyakhiwa.Yonke imimandla ye-chromatin evulekileyo inokufumaneka phantsi kwemeko yendawo yexesha elithile, engaphelelanga kuphela kwiindawo ezibophelelayo ze-transcription factor, okanye ummandla othile we-histone modified.
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UMelo oNcitshisiweyo we-Bisulfite Sequencing (RRBS)
Uphando lwe-DNA methylation luhlala luyisihloko esishushu kuphando lwezifo, kwaye luhambelana ngokusondeleyo ne-gene expression kunye ne-pheno-typic traits.I-RRBS yindlela echanekileyo, esebenzayo kunye neyoqoqosho yophando lwe-DNA methylation.Ukutyetyiswa komgqugquzeli kunye nemimandla yesiqithi se-CpG nge-enzymatic cleavage (Msp I), idityaniswe nokulandelelana kwe-Bisulfite, ibonelela ngesisombululo esiphezulu se-DNA methylation yokufumanisa.
Iqonga: Illumina NovaSeq 6000
