Iimveliso

I-DNA / RNA Ukulandelelana - i-Nanopore Sequencer

I-DNA / i-RNA Ukulandelelana - i-Nanopore Sequencer Umfanekiso obonakalayo

Ukulandelelana kwe-ONT yi-molecule enye ye-real-time real-time signal sequencing technology esekelwe kwi-nanopores, umgaqo wokulandelelana kweqonga ngalinye liyafana.I-DNA/RNA enemisonto ephindwe kabini iya kubophelela kwiprotein ye-nanoporous efakwe kwi-biofilm kwaye ikhulule phantsi kwesikhokelo seprotein yemoto, phantsi kwesenzo somohluko wombane kumacala omabini e-biofilm, imitya ye-DNA/RNA idlula kwiprotein yesitishi se-nanopore kwindawo ethile. izinga.Ngenxa yokungafani kweempawu zeekhemikhali zeziseko ezahlukeneyo kwi-DNA / i-RNA strand, xa isiseko esisodwa okanye i-molecule ye-DNA idlula kwi-channel ye-nanopore, iya kubangela ukuguqulwa kweempawu zombane ezahlukeneyo.Ngokufumanisa kunye nokuhambelana nale miqondiso, iintlobo zesiseko ezihambelanayo zingabalwa, kwaye ukubonwa kwexesha langempela lokulandelelana kunokugqitywa.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Iziphumo zeDemo

IiNkcukacha zeNkonzo Iimpawu

Iqonga	Ubungakanani beThala leeNcwadi	Ukukhutshwa kweDatha yeThiyori (NgeSeli)	Ukuchaneka kwesiseko esinye	Usetyenziso
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Iseli	85-92%	Ukufowuna kwe-SV, iDe novo, ukulandelelana kobude obupheleleyo, i-Iso-Seq, i-Gene annotation, Ukufunyanwa kwe-DNA methylation

Iinkonzo eziluncedo

● Ngaphezulu kweminyaka emi-5 yamava kwi-PacBio yokulandelelana kweqonga kunye namawaka eeprojekthi ezivaliweyo ezineentlobo ezahlukeneyo.
● I-BMKGENE ngumlingani osemthethweni we-Oxford Nanopore, kunye ne-platform ye-RNA / DNA certification.
● Kukho imifuziselo yesiqhelo yabalandelelanisi abanezixhobo ezipheleleyo kunye ne-throughput yolandelelwano olwaneleyo.
● Ngokusekelwe kwiqonga leNanopore, ngaphezu kwezilwanyana ze-10 kunye nophando lweDenovo lwezityalo luye lwapapashwa kwiijenali ezidumileyo kumazwe ngamazwe.

IiMfuno zeSampulu

Uhlobo lweSampuli

Isixa

Ugxininiso(Qubit ®)

Umthamo

Ubunyulu

Abanye

Genomic DNA

Kuxhomekeke kwiMfuno yeDatha

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

Incopho ecacileyo ye-260 nm, akukho kungcoliseko

Ugxininiso kufuneka lulinganiswe ngeQubit kunye neQubit / Nanopore ≤ 2

Iyonke i-RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5;akukho kungcoliseko

Ixabiso le-RIN ≥7.5

Ukuhanjiswa kwenkonzo

Ukulungiselela isampuli

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Uhlalutyo lwedatha

Unikezelo lweprojekthi

Ngaphambili: I-DNA / RNA Ukulandelelana - Illumina Sequencer

Okulandelayo: I-Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

UVavanyo lweMgangatho weDatha yeSampuli yeDNA

Itheyibhile 1. Izibalo kwidatha ecocekileyo.

BMKID	eluhlazaSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	cleanN50Len	cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9