Ukuthelekisa iGenomics
Iinkonzo eziluncedo
● Ipakethe yocazululo olubanzi, equlathe uhlalutyo olusibhozo olufuneka ngokuqhelekileyo
● Ukuthembeka okuphezulu kuhlalutyo oluneenkcukacha kunye nokutolika ngokulula kwiziphumo
● Amanani ayilwe kakuhle alungele ukupapashwa
● Iqela le-bioinformatics elinezakhono eziphezulu lizalisekisa iimfuno ezahlukeneyo zohlalutyo lomntu
● Ixesha elifutshane lokujika-jika ngokuchaneka okuphezulu kuhlalutyo
● Amava amaninzi aneemeko eziyimpumelelo ezingaphezu kwama-90 ezinempembelelo eyongezelelweyo epapashiweyo engaphezu kwama-900
IiNgcaciso zeNkonzo
| Ixesha eliqikelelweyo lokujika | Inani leentlobo | Uhlalutya |
| Iintsuku zokusebenza ezingama-30 | 6 - 12 | Ukuhlanganiswa kosapho lweGene Ukwandiswa kosapho lweGene kunye nokuncipha Ukwakhiwa komthi wePhylogenetic Uqikelelo lwexesha lokwahluka (ulungelelwaniso lweFossil luyafuneka) Ixesha lofakelo lwe-LTR (Yezityalo) Uphindaphindo lwe-genome epheleleyo (Yezityalo) Uxinzelelo olukhethiweyo Uhlalutyo lwe-Synteny |
Uhlalutyo lwe-Bioinformatics
● Intsapho yakwaGene
● IPhylogenetics
● Ixesha lokuhlukana
● Uxinzelelo olukhethekileyo
● Uhlalutyo lwentsingiselo
IiMfuno zeSampulu kunye nokuhanjiswa
IiMfuno zeSampulu:
I-Tissue okanye i-DNA yolandelelwano lwe-genome kunye nokudibanisa
Yezicubu
| Iintlobo | Izicubu | Uvavanyo | I-PacBio CCS |
| Isilwanyana | Izicubu ze-Visceral | 0.5 ~ 1g | ≥ 3.5g |
| Izicubu zezihlunu | |||
| ≥ 5.0g | |||
| ≥ 5.0mL | |||
| Igazi leMammalian | |||
| ≥ 0.5mL | |||
| Inkukhu / Igazi lentlanzi | |||
| Isityalo | Igqabi Elitsha | 1 ~ 2g | ≥ 5.0g |
| Ipetali/Isiqu | 1 ~ 2g | ≥ 10.0g | |
| Ingcambu/Imbewu | 1 ~ 2g | ≥ 20.0g | |
| Iiseli | Iseli Cultured | - | ≥ 1 x 108 |
Idatha
Iifayile zolandelelwano lweGenome(.fasta) kunye neefayile zenkcazo (.gff3) zeentlobo ezinxulumene ngokusondeleyo
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
*Iziphumo zedemo eziboniswe apha zonke zivela kwiigenomes ezipapashwe ngeBiomarker Technologies
I-1.LTR ifake uqikelelo lwexesha: Inani eliboniswe usasazo olulodwa lwe-bimodal ngamaxesha okufakwa kwe-LTR-RTs kwi-Weining rye genome, xa kuthelekiswa nezinye iintlobo.Eyona ncopho yakutshanje yavela malunga ne-0.5 yezigidi zeminyaka eyadlulayo.
ULi Guang et al.,Imfuzo yendalo, 2021
I-2.I-Phylogeny kunye nohlalutyo lwentsapho ye-gene kwi-chayote (i-Sechium edule) : Ngokuhlalutya i-chayote kunye nezinye iintlobo ze-13 ezinxulumene ne-gene yentsapho, i-Chayote yafunyanwa inxulumene kakhulu ne-snake gourd (i-Trichosanthes anguina).I-Chayote ethathwe kwintlavulo yenyoka ejikeleze i-27-45 Mya kunye nokuphindaphinda kwe-genome yonke (WGD) yabonwa kwi-chayote kwi-25±4 Mya, isiganeko sesithathu se-WGD kwi-cucuibitaceae.
Fu A et al.,Uphando lweHorticulture, 2021
3.Uhlalutyo lwe-Synteny: Ezinye iijini ezinxulumene ne-phytohormones ekuphuhliseni iziqhamo zifunyenwe kwi-chayote, i-snake gourd kunye ne-squash.Unxulumano phakathi kwe chayote kunye ne squash luphezulu kancinci kunolo luphakathi kwe chayote kunye ne snake gourd.
Fu A et al.,Uphando lweHorticulture, 2021
Uhlalutyo lwentsapho ye-4.Gene: Ukutyetyiswa kwe-KEGG kwi-gene yentsapho yokwandiswa kunye nokunyanzeliswa kwe-G.thurberi kunye ne-G.davidsonii genomes eboniswe ukuba i-steroid biosynthesis kunye ne-brassinosteroid biosynthesis enxulumene nohlobo lwandisiwe.
Yang Z et al.,Ibhayoloji ye-BMC, 2021
5.Uhlalutyo lwe-genome oluphindwayo lulonke: Uhlalutyo lwe-4DTV kunye ne-Ks lokusasazwa luboniswe isiganeko sokuphindaphinda kwe-genome.Incopho ye-intraspecies ebonisiweyo yeziganeko eziphindaphindayo.Incopho ye-interspecies ebonisiweyo yeziganeko.Uhlalutyo lubonise ukuba xa kuthelekiswa nezinye iintlobo ezintathu ezinxulumene ngokusondeleyo, iO.
URao G et al.,Uphando lweHorticulture, 2021
Ityala le-BMK
I-Rose ngaphandle kwe-prickle: ulwazi lwe-genomic oludityaniswe nokulungelelanisa ukufuma
Ipapashiwe: Uphononongo lweNzululwazi yeSizwe, 2021
Iqhinga lokulandelelanisa:
'eBasyeIngenameva' (R.Wichurainan) genome:
Malunga.93 X PacBio + malunga.90 X Nanopore + 267 X Illumina
Iziphumo eziphambili
I-1.Umgangatho ophezulu we-R.wichuraiana genome wakhiwa kusetyenziswa iindlela zokulandelelanisa ixesha elide, ezivelisa i-assembly ye-530.07 Mb (Ubungakanani obuqikelelweyo be-genome malunga ne-525.9 Mb nge-cytometry yokuhamba kunye ne-525.5 nge-genome survey; I-Heterozygosity yayimalunga ne-1.03%).Amanqaku eBUSCO aqikelelwa kwi-93.9%.Xa kuthelekiswa ne "Old blush" (haploOB), umgangatho kunye nokuphelela kwale genome kwaqinisekiswa ngokuchaneka kwesiseko esisodwa kunye nesalathisi sendibano ye-LTR (LAI = 20.03).I-R.wichuraiana genome iqulethe i-32,674 ye-protein coding genes.
2.Uhlalutyo oludibeneyo lwe-Multi-omics, olubandakanya i-genomics ethelekisayo, i-transcriptomics, uhlalutyo lwe-QTL yofuzo lwabantu, lubonise i-speciation ebalulekileyo phakathi kwe-R. wichuraiana kunye ne-Rosa chinensis.Kwakhona, ukwahluka kwentengiselwano yeegenes ezinxulumeneyo kwi-QTL bekunokwenzeka ukuba inxulunyaniswe ne-stem prickle patterning.
Ucazululo lothelekiso lwe-genomics phakathi kwe-Basye; i-Thornless kunye ne-Rosa chinensis kubandakanya uhlalutyo lwe-synteny, iqoqo losapho lwemfuza, uhlalutyo lolwandiso kunye nokucutheka, kutyhilwe inani elikhulu leyantlukwano, ehambelana neempawu ezibalulekileyo kwiirozi.Ukwandiswa okukodwa kwi-NAC kunye nosapho lwemfuza ye-FAR1/FRS bekunokwenzeka ukuba inyanyaniswe nokuchasa indawo emnyama.
Uhlalutyo lothelekiso lwe-genomics phakathi kwe-BT kunye ne-haploOB genomes.
Zhong, M., et al."I-Rose ngaphandle kwe-prickle: ulwazi lwe-genomic olunxulunyaniswa nokulungelelanisa ukufuma"Uphononongo lweNzululwazi yeSizwe, 2021;, nwb092.
