● Ufundo olude luveza ulandelelwano olupheleleyo lwe-16S/18S/ITS
● Isiseko esichanekileyo sokufowuna kunye nolandelelwano lwemodi ye-PacBio CCS
● Isisombululo senqanaba leentlobo kwinkcazo ye-OTU/ASV
● I-QIIME2 yamva nje yokuhlalutya ukuhamba nohlalutyo olwahlukeneyo ngokwemigaqo yogcino-lwazi, inkcazo, i-OTU/ASV.
● Isetyenzwa kwizifundo zoluntu ezahlukeneyo zemicrobial
● I-BMK inamava abanzi ngeesampulu ezingaphezulu kwe-100,000 ngonyaka, ezigquma umhlaba, amanzi, igesi, udaka, ilindle, amathumbu, ulusu, umhluzi wokubila, izinambuzane, izityalo, njl.
● I-BMKCloud iququzelele ukutolikwa kwedatha equlethe i-45 yezixhobo zokuhlalutya zomntu
UkulandelelanisaIqonga | Ithala leencwadi | Idatha ecetyiswayo | Ixesha lokuguqula |
I-PacBio Sequel II | SMRT-intsimbi | 5K/10K/20K iithegi | 44 Iintsuku zokusebenza |
● Ulawulo lomgangatho wedatha ekrwada
● I-OTU clustering/De-noise(ASV)
● Inkcazo ye-OTU
● Ukwahluka kweAlpha
● Ukwahluka kweBeta
● Uhlalutyo lwamaqela ngamaqela
● Uhlalutyo lombutho ngokuchasene nemiba yovavanyo
● Uqikelelo lwemfuza yokusebenza
KubaIzicatshulwa zeDNA:
Uhlobo lweSampuli | Isixa | Ukugxininisa | Ubunyulu |
Izicatshulwa zeDNA | > 1 μg | > 20 ng/μl | OD260/280= 1.6-2.5 |
Ngeesampuli zokusingqongileyo:
Uhlobo lwesampuli | Inkqubo yesampulu ecetyiswayo |
Umhlaba | Isixa sesampulu: malunga.5 g;Into eseleyo ebunayo kufuneka isuswe kumphezulu;Gaya iinqununu ezinkulu kwaye udlule kwi-2 mm filter;Iisampulu ze-Aliquot kwi-EP-tube engcolileyo okanye i-cyrotube yogcino. |
Ilindle | Isixa sesampulu: malunga.5 g;Qokelela kwaye iisampulu ze-aliquot kwi-EP-tube engcolileyo okanye i-cryotube yokubhukisha. |
Iziqulatho zamathumbu | Iisampuli kufuneka ziqwalaselwe phantsi kwemeko ye-aseptic.Hlamba izicubu eziqokelelweyo nge-PBS;I-Centrifuge i-PBS kwaye uqokelele i-precipitant kwi-EP-tubes. |
Udaka | Isixa sesampulu: malunga.5 g;Qokelela kwaye isampuli ye-aliquot ye-sludge kwi-EP-tube engcolileyo okanye i-cryotube yokugcinelwa |
Amanzi | Kwisampulu enesixa esilinganiselweyo se-microbial, njengamanzi epompo, amanzi omthombo, njl., Qokelela ubuncinci be-1 L yamanzi kwaye udlule kwi-0.22 μm yokucoca ukutyebisa i-microbial kwi-membrane.Gcina inwebu kwityhubhu enyumba. |
Ulusu | Khulula ngononophelo umphezulu wolusu ngeswab yomqhaphu engcolileyo okanye iblade yotyando kwaye uyibeke kwityhubhu engenanto. |
Umkhenkce iisampulu kulwelo nitrogen iiyure 3-4 kwaye ugcine kulwelo nitrogen okanye -80 degree ukuya ugcino ixesha elide.Isampulu yokuthumela ngenqanawe eyomileyo iyafuneka.
I-1.Izinga le-Annotation ye-V3 + V4 (Illumina)-based microbial community profiling vs. Full-length (PacBio)-based profiling.
(Idatha yeeprojekthi ezingama-30 ezichongwe ngokungacwangciswanga ziye zafakwa kwizibalo)
2.Ireyithi yenkcazo yolandelelwano lweamplicon yobude obupheleleyo kwinqanaba leentlobo ezahlukeneyo zesampulu
3.Ukuhanjiswa kweentlobo
4.Umthi wePhylogenetic
Ityala le-BMK
Ukuvezwa kwe-arsenic kubangela umonakalo othintela amathumbu kunye nokulandelwa kokusebenza kwe-gut-liver axis ekhokelela ekudumbeni kunye ne-pyroptosis yesibindi kumadada.
Ipapashiwe:Inzululwazi yeNdawo yonke,2021
Iqhinga lokulandelelanisa:
Iisampulu: Ukulawula vs 8 mg / kg ATO evezwe iqela
Ukulandelelanisa isivuno sedatha: 102,583 ulandelelwano lwe-CCS ekrwada iyonke
Ulawulo: 54,518 ± 747 CCS esebenzayo
I-ATO-eveziweyo : 45,050 ± 1675 i-CCS esebenzayo
Iziphumo eziphambili
Ukwahluka kweAlpha:Ukuvezwa kwe-ATO kutshintshe kakhulu ubutyebi be-intestinal microbial kunye nokwahluka kwamadada.
Uhlalutyo lwe-Metastats:
Kwinqanaba le-phylum: I-2 ibhaktheriya phyla ifunyenwe kuphela kumaqela olawulo
Kwinqanaba le-genus: I-6 genera ifunyenwe yahluke kakhulu kubuninzi obunxulumene
Kwinqanaba leentlobo: Iindidi ezingama-36 zachongwa zizonke, kunye ne-6 yazo yahluke kakhulu kubuninzi be-relavive
Isalathiso
Thingholm, LB , et al."Abantu abatyebileyo abanesifo seswekile kunye nabangenaso iSifo seswekile sesi-2 babonisa isakhono esiSebenzayo seThumbu leMicrobial."Umamkeli weSeli kunye neMicrobe26.2(2019).