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Wholesale Sequencing Strategy - Full-length mRNA sequencing-Nanopore – Biomarker

Service Details

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Wholesale Sequencing Strategy - Full-length mRNA sequencing-Nanopore – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing

ü Transcript identification

ü Alternative splicing

ü Expression quantification in gene level and isoform level

ü Differential expression analysis

ü Function annotation and enrichment (DEGs and DETs)

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Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel.	For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥40 ng/μl;Total ≥ 1 μg

Tissue: Weight(dry): ≥1 g

*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷

*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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With our loaded encounter and considerate services, we have now been recognized as a trustworthy supplier for lots of worldwide consumers for Wholesale Sequencing Strategy - Full-length mRNA sequencing-Nanopore – Biomarker , The product will supply to all over the world, such as: Bahamas , Singapore , Islamabad , With a state-of-the-art comprehensive marketing feedback system and 300 skilled workers' hard work, our company has developed all kinds of products ranging from high class, medium class to low class. This whole selection of fine products offers our customers different choices. Besides, our company sticks to high quality and reasonable price, and we also offer good OEM services to many famous brands.

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This supplier's raw material quality is stable and reliable, has always been in accordance with the requirements of our company to provide the goods that quality meet our requirements.

By Doris from Thailand - 2017.09.22 11:32

The company can keep up with the changes in this industry market, product updates fast and the price is cheap, this is our second cooperation, it's good.

By Caroline from Hanover - 2018.04.25 16:46