Wholesale Sequencing Strategy - circRNA sequencing – Illumina – Biomarker
Wholesale Sequencing Strategy - circRNA sequencing – Illumina – Biomarker Detail:
Service Advantages
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Purity | Integrity | Contamination | Amount |
| OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; | For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation | Limited or no protein or DNA contamination shown on gel. | Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.
Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
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By Christina
from Miami
- 2018.02.08 16:45
Although we are a small company, we are also respected. Reliable quality, sincere service and good credit, we are honored to be able to work with you!
By Camille
from Johor
- 2018.12.30 10:21
