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Well-designed Rna Sequencing Cost - De novo Full-length Transcriptome sequencing -PacBio – Biomarker


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Well-designed Rna Sequencing Cost - De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing
 
ü Transcript identification
 
ü Sequence structure
 
ü Expression Quantification
 
ü Function Annotation

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Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Amount Total ≥ 3 μg;  Conc. ≥ 300 ng/μl;  Volumn ≥ 10 μl
Integrity For plants: RIN ≥ 7.5; For animals: RIN ≥ 8;  28S/18S ≥ 1.0;  Limited or no baseline elevation
Purity OD260/280 ≥ 1.8;  OD260/230 ≥ 1.0;  Clear peak at 260 nm
Note Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level.

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×106 – 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services


Product detail pictures:

Well-designed Rna Sequencing Cost -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures

Well-designed Rna Sequencing Cost -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures

Well-designed Rna Sequencing Cost -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures

Well-designed Rna Sequencing Cost -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures


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