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Well-designed Metagenomics - De novo Full-length Transcriptome sequencing -PacBio – Biomarker


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Well-designed Metagenomics - De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing
 
ü Transcript identification
 
ü Sequence structure
 
ü Expression Quantification
 
ü Function Annotation

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Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Amount Total ≥ 3 μg;  Conc. ≥ 300 ng/μl;  Volumn ≥ 10 μl
Integrity For plants: RIN ≥ 7.5; For animals: RIN ≥ 8;  28S/18S ≥ 1.0;  Limited or no baseline elevation
Purity OD260/280 ≥ 1.8;  OD260/230 ≥ 1.0;  Clear peak at 260 nm
Note Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level.

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×106 – 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services


Product detail pictures:

Well-designed Metagenomics -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures

Well-designed Metagenomics -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures

Well-designed Metagenomics -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures

Well-designed Metagenomics -
 De novo Full-length Transcriptome sequencing -PacBio – Biomarker detail pictures


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  • This company has the idea of "better quality, lower processing costs, prices are more reasonable", so they have competitive product quality and price, that's the main reason we chose to cooperate.
    5 Stars By Wendy from Lithuania - 2017.08.18 18:38
    Product variety is complete, good quality and inexpensive, the delivery is fast and transport is security, very good, we are happy to cooperate with a reputable company!
    5 Stars By Naomi from Swansea - 2017.06.16 18:23

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