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Sequloana se le seng sa RNA Sequencing
Tsoelo-pele ea ho nka sele e le 'ngoe le mokhoa oa ho haha laebrari ka bomong ho kopanngoa le tatellano e phahameng e lumellang lithuto tsa liphatsa tsa lefutso motheong oa sele-by-cell.E etsa hore ho be le tlhahlobo e tebileng le e felletseng ea litsamaiso tsa lisele tse rarahaneng, moo haholo-holo e qobang ho pata liphapang tsa bona ka ho nka karolelano ea lisele tsohle.
Leha ho le joalo, lisele tse ling ha li tšoanelehe ho etsoa ho emisoa ha sele e le 'ngoe, kahoo mekhoa e meng ea ho lokisa sampole - ho ntšoa ha nucleus ho tsoa liseleng hoa hlokahala, ke hore, nucleus e ntšoa ka kotloloho ho lisele kapa lisele ebe e lokisetsoa ho emisoa ha nucleus e le 'ngoe bakeng sa motho a le mong. tatellano ea lisele.
BMK e fana ka ts'ebeletso ea tatellano ea 10× Genomics ChromiumTM e thehiloeng ho sele e le 'ngoe ea RNA.Ts'ebeletso ena e sebelisitsoe haholo lithutong tse amanang le mafu, joalo ka phapang ea lisele tsa 'mele oa ho itšireletsa mafung, tumor heterogeneity, nts'etsopele ea lisele, joalo-joalo.
Spatial transcriptome chip: 10 × Genomics
Sethala: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Li-Spatial transcriptomics li ka pele ho boqapi ba mahlale, li matlafatsa bafuputsi ho batlisisa mekhoa e rarahaneng ea liphatsa tsa lefutso ka har'a lisele ha ba ntse ba boloka maemo a bona a sebaka.Har'a li-platform tse fapaneng, BMKGene e hlahisitse BMKManu S1000 Spatial Transcriptome Chip, e ithorisa katharollo e ntlafalitsoengea 5µM, ho fihla ho subcellular range, le ho nolofalletsalitlhophiso tsa tharollo ea maemo a mangata.Chip ea S1000, e nang le matheba a ka bang limilione tse 2, e sebelisa li-microwell tse koahetsoeng ka lifaha tse nang le lisebelisoa tsa ho hapa tse nang le barcode.Laeborari ea cDNA, e ntlafalitsoeng ka li-barcode tsa sebaka, e lokisoa ho tsoa ho chip ea S1000 mme ka mor'a moo e hlophisoa sethaleng sa Illumina NovaSeq.Motsoako oa disampole tse nang le barcoded tsa sebaka le li-UMI li netefatsa ho nepahala le ho nepahala ha data e hlahisitsoeng.Tšobotsi e ikhethang ea BMKManu S1000 chip e itšetlehile ka ho feto-fetoha ha eona, e fana ka litlhophiso tsa maemo a mangata tse ka hlophisoang hantle ho lisele tse fapaneng le maemo a lintlha.Ho ikamahanya le maemo hona ho beha chip e le khetho e ikhethang bakeng sa lithuto tse fapaneng tsa transcriptomics tsa sebaka, e netefatsang kopano e nepahetseng ea sebaka ka lerata le fokolang.
Ba sebelisa chip ea BMKManu S1000 le mahlale a mang a transcriptomics a sebaka, bafuputsi ba ka fumana kutloisiso e betere ea mokhatlo oa sebaka sa lisele le litšebelisano tse rarahaneng tsa limolek'hule tse etsahalang ka har'a lisele, ho fana ka leseli la bohlokoa mabapi le lits'ebetso tsa ts'ebetso ea bioloji libakeng tse fapaneng, ho kenyeletsoa. oncology, neuroscience, tsoelopele ea baeloji, immunology le lithuto tsa botanical.
Platform: BMKManu S1000 chip le Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics ke theknoloji e tsoetseng pele e lumellang bafuputsi ho etsa lipatlisiso tsa mefuta ea liphatsa tsa lefutso ka har'a lisele ha ba ntse ba boloka maemo a bona a sebaka.Sethala se le seng se matla sebakeng sena ke 10x Genomics Visium e kopantsoeng le tatellano ea Illumina.Molao-motheo oa 10X Visium o holim'a chip e khethehileng e nang le sebaka se khethiloeng sa ho hapa moo likarolo tsa lisele li behoang teng.Sebaka sena sa ho ts'oaroa se na le libaka tse nang le barcode, e 'ngoe le e' ngoe e tsamaellana le sebaka se ikhethileng ka har'a thishu.Limolek'hule tsa RNA tse hapuoeng ho tsoa liseleng ebe li ngotsoe ka li-identifiers tse ikhethang tsa limolek'hule (UMIs) nakong ea ts'ebetso ea ho fetolela ka morao.Libaka tsena tse nang le li-barcode le li-UMI li thusa ho etsa 'mapa o nepahetseng oa sebaka le ho lekanya liphatsa tsa lefutso ka qeto ea sele e le 'ngoe.Motsoako oa disampole tse nang le barcoded tsa sebaka le li-UMI li netefatsa ho nepahala le ho nepahala ha data e hlahisitsoeng.Ka ho sebelisa theknoloji ena ea Spatial Transcriptomics, bafuputsi ba ka fumana kutloisiso e tebileng ea mokhatlo oa sebaka sa lisele le litšebelisano tse rarahaneng tsa limolek'hule tse hlahang ka har'a lisele, tse fanang ka kutloisiso ea bohlokoa ka mekhoa ea mekhoa ea likokoana-hloko likarolong tse ngata, ho akarelletsa le oncology, neuroscience, tsoelo-pele ea biology, immunology. , le lithuto tsa botanical.
Sethala: 10X Genomics Visium le Illumina NovaSeq
