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BSA
Sethala sa Bulked Segregant Analysis se na le tlhahlobo e tloaelehileng ea mohato o le mong le tlhahlobo e tsoetseng pele e nang le maemo a ikhethileng a paramente.BSA ke mokhoa o sebelisoang ho tsebahatsa matšoao a lefutso a amanang le phenotype.Ts'ebetso e kholo ea BSA e na le: 1. ho khetha lihlopha tse peli tsa batho ba nang le phenotypes tse hanyetsanang haholo;2. ho kopanya DNA, RNA kapa SLAF-seq(E ntlafalitsoe ke Biomarker) ea batho bohle ho etsa bongata bo boholo ba DNA;3. ho khetholla tatellano e fapaneng khahlanong le genome ea litšupiso kapa lipakeng, 4. ho bolela esale pele libaka tse amanang le mokhethoa ka ED le SNP-index algorithm;5. Tlhahlobo ea ts'ebetso le ho ntlafatsa liphatsa tsa lefutso libakeng tsa bo-nkgetheng, joalo-joalo. Meepo e tsoetseng pele haholoanyane litabeng tse kenyeletsang tlhahlobo ea liphatsa tsa lefutso le moralo oa pele le tsona lia fumaneha.
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Amplicon (16S/18S/ITS)
Sethala sa Amplicon (16S/18S/ITS) se entsoe ka boiphihlelo ba lilemo tsa tlhahlobo ea projeke ea mefuta-futa ea likokoana-hloko, e nang le tlhahlobo e tloaelehileng ea mantlha le tlhahlobo ea motho ka mong: tlhahlobo ea mantlha e akaretsa litaba tsa tlhahlobo e akaretsang ea lipatlisiso tsa hajoale tsa likokoana-hloko, litaba tsa tlhahlobo li ruile ebile li na le botlalo, le liphetho tsa tlhahlobo li hlahisoa ka mokhoa oa litlaleho tsa morero;Litaba tsa tlhahlobo ea motho ka mong li fapane.Mehlala e ka khethoa 'me liparamente li ka hlophisoa ka mokhoa o bonolo ho latela tlaleho ea tlhahlobo ea mantlha le sepheo sa lipatlisiso, ho fihlela litlhoko tsa motho ka mong.Sistimi ea ts'ebetso ea Windows, e bonolo ebile e potlakile.
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Liphatsa tsa lefutso tsa ho iphetola ha lintho
Evolutionary genetics ke ts'ebeletso e hlophisitsoeng ea tatelano e etselitsoeng ho fana ka tlhaloso e felletseng mabapi le tlhaiso-leseling ea ho iphetola ha lintho ea thepa e fanoeng e ipapisitseng le liphatsa tsa lefutso, ho kenyeletsoa SNPs, InDels, SVs le CNVs.E fana ka tlhahlobo eohle ea motheo e hlokahalang bakeng sa ho hlalosa liphetoho tsa ho iphetola ha lintho le likarolo tsa lefutso tsa baahi, joalo ka sebopeho sa baahi, mefuta-futa ea liphatsa tsa lefutso, likamano tsa phylogeny, joalo-joalo. E boetse e na le lithuto tsa phallo ea liphatsa tsa lefutso, tse matlafatsang khakanyo ea boholo bo sebetsang ba baahi, nako ea ho fapana.
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Papiso ea Genomics
Papiso ea genomics ha e le hantle e bolela ho bapisa tatellano e felletseng ea liphatsa tsa lefutso le libopeho tsa mefuta e fapaneng.Taeo ena e ikemiselitse ho senola phetoho ea mefuta, tšebetso ea liphatsa tsa lefutso, mokhoa oa taolo ea liphatsa tsa lefutso maemong a genome ka ho tsebahatsa libopeho tsa tatelano le likarolo tse bolokileng kapa tse arohanyang mefuta e fapaneng.Boithuto bo tloaelehileng ba ho bapisa liphatsa tsa lefutso bo kenyelletsa tlhahlobo ea liphatsa tsa lefutso, nts'etsopele ea ho iphetola ha lintho, phetiso e felletseng ea genome, khatello ea khetho, jj.
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Liphatsa tsa lefutso tsa ho iphetola ha lintho
Sethala sa tlhahlobo ea liphatsa tsa lefutso tsa baahi le kholo ea ho iphetola ha lintho se thehiloe motheong oa boiphihlelo bo bongata bo bokelletsoeng ka har'a sehlopha sa BMK R&D ka lilemo.Ke sesebelisoa se bonolo sa basebelisi haholo-holo bakeng sa bafuputsi ba sa ithuteng haholo ho bioinformatics.Sethala sena se thusa tlhahlobo ea mantlha e amanang le liphatsa tsa lefutso tse amanang le ho aha lifate tsa phylogenetic, tlhahlobo ea ho se lekane ha khokahano, tlhahlobo ea mefuta-futa ea liphatsa tsa lefutso, tlhahlobo e ikhethileng, tlhahlobo ea kamano, PCA, tlhahlobo ea sebopeho sa baahi, jj.
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Hi-C e thehiloeng ho Genome Assembly
Hi-C ke mokhoa o etselitsoeng ho ts'oara tlhophiso ea chromosome ka ho kopanya litšebelisano tse thehiloeng ho proximity le tatellano ea maemo a holimo.Ho lumeloa hore matla a litšebelisano tsena a amana hampe le sebaka sa 'mele ho li-chromosome.Ka hona, lintlha tsa Hi-C li ka tataisa ho kopanya, ho hlophisa le ho tsamaisa tatellano e kopaneng ho genome e hlophisitsoeng le ho tiisa tseo palo e itseng ea li-chromosome.Theknoloji ena e matlafatsa kopano ea liphatsa tsa lefutso tsa boemo ba chromosome ha ho se na 'mapa oa lefutso o thehiloeng ho baahi.Genome e 'ngoe le e' ngoe e hloka Hi-C.
Sethala: Illumina NovaSeq Platform / DNBSEQ
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Semela / Phoofolo De Novo Genome Sequencing
Ho tloha Novotatellano e bolela kaho ea mofuta oohle oa lefutso ho sebelisoa theknoloji ea tatelano, mohlala, PacBio, Nanopore, NGS, joalo-joalo, ha ho se na genome.Ntlafatso e tsotehang ea bolelele ba ho bala ba theknoloji ea tatellano ea moloko oa boraro e tlisitse menyetla e mecha ea ho bokella liphatsa tsa lefutso tse rarahaneng, tse kang tse nang le heterozygosity e phahameng, karo-karolelano e phahameng ea libaka tse pheta-phetoang, li-polyploids, joalo-joalo. ho rarolla lintlha tse ipheta-phetang, libaka tse nang le litaba tsa GC tse sa tloaelehang le libaka tse ling tse rarahaneng haholo.
Sethala: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq Platform
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Sequencing ea Exome ea Motho e Felletseng
Whole exome sequencing (WES) e nkuoa e le leano la tatelano le baballang chelete bakeng sa ho tseba liphetoho tse bakang mafu.Leha li-exons li nka hoo e ka bang 1.7% ea genome kaofela, e emela boemo ba tšebetso ea protheine ka kotloloho.Liphatseng tsa lefutso tsa motho, ho tlalehiloe hore ho feta 85% ea liphetoho tse amanang le mafu li etsahala sebakeng sa protheine ea likhoutu.
BMKGENE e fana ka lits'ebeletso tse felletseng le tse tenyetsehang tsa tatellano ea batho ka kakaretso ka maano a fapaneng a ho hapa a fumanehang ho fihlela lipheo tse fapaneng tsa lipatlisiso.
Sethala: Illumina NovaSeq Platform
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, haholo-holo ho baahi ba bangata, ke mohato oa bohlokoa lithutong tsa liphatsa tsa lefutso, tse fanang ka motheo oa liphatsa tsa lefutso bakeng sa ho sibolloa ha liphatsa tsa lefutso, tlhahlobo ea ho iphetola ha lintho, joalo-joalo Ho e-na le tatellano e tebileng ea liphatsa tsa lefutso, ho fokotseha ha tatellano ea liphatsa tsa lefutso (RRGS). ) e hlahisoa ho fokotsa litšenyehelo tsa tatellano ea sampuli ka 'ngoe, ha ho ntse ho boloka ts'ebetso e utloahalang ho sibolloeng ha lets'oao la lefutso.Hangata sena se finyelloa ka ho ntša sekhechana sa lithibelo ka har'a boholo bo fanoeng, se bitsoang laeborari ea boemeli bo fokolitsoeng (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) ke leano le iketselitseng la SNP genotyping ka kapa ntle le genome ea referense.
Sethala: Illumina NovaSeq Platform -
Illumina le BGI
Theknoloji ea tatellano ea Illumina, e ipapisitseng le Sequencing by Synthesis (SBS), ke popontšuoa ea lefatše ka bophara ea NGS, e ikarabellang bakeng sa ho hlahisa ho feta 90% ea data ea tatellano ea lefatše.Molao-motheo oa SBS o kenyelletsa ho nahana ka li-terminator tse fetolehang tse ngotsoeng ka fluorescent ha dNTP ka 'ngoe e eketsoa, 'me ka mor'a moo e khomaretsoe ho lumella ho kenyelletsoa ha setsi se latelang.Ka li-dNTP tse 'ne tse ka khutlisetsoang ka li-terminator tse teng nakong e 'ngoe le e 'ngoe ea tatellano, tlholisano ea tlhaho e fokotsa leeme la kenyelletso.Theknoloji ena e feto-fetohang e ts'ehetsa lilaebrari tse baloang ha motho a le mong le tse peli, tse sebetsanang le mefuta e mengata ea likopo tsa genomic.Bokhoni ba tatellano e phahameng ea Illumina le ho nepahala ha eona bo e beha e le motheo oa lipatlisiso tsa genomics, e matlafatsa bo-ramahlale ho manolla boqhetseke ba liphatsa tsa lefutso ka lintlha tse ke keng tsa lekanngoa le bokhabane.
DNBSEQ, e ntlafalitsoeng ke BGI, ke theknoloji e 'ngoe e ncha ea NGS e khonneng ho fokotsa litšenyehelo tsa tatellano le ho eketsa sephetho.Tokiso ea lilaebrari tsa DNBSEQ e kenyelletsa ho arohana ha DNA, ho lokisoa ha ssDNA le ho phahamisa selikalikoe ho fumana li-nanoballs tsa DNA (DNB).Ka mor'a moo, tsena li laeloa holim'a sebaka se tiileng 'me ka mor'a moo li hlophisoa ke motsoako oa Probe-Anchor Synthesis (cPAS).
Ts'ebeletso ea rona ea tatellano ea laeborari e entsoeng esale pele e thusa bareki ho hlophisa lilaeborari tsa bona tsa tatellano ho tsoa mehloling e fapaneng (mRNA, genome eohle, amplicon, hara tse ling).Kamora moo, lilaebrari tsena li ka romelloa litsing tsa rona tsa tatellano bakeng sa taolo ea boleng le tatellano ho li-platform tsa Illumina kapa tsa BGI.
