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Metatranscriptome Sequencing
Metatranscriptome sequencing e supa liphatsa tsa lefutso tsa likokoana-hloko (ka bobeli eukaryotes le prokaryotes) ka har'a tikoloho ea tlhaho (e leng mobu, metsi, leoatle, mantle le mala.) Haholo-holo, litšebeletso tsena li u lumella ho fumana boitsebiso bo feletseng ba liphatsa tsa lefutso tsa lichaba tse rarahaneng tsa likokoana-hloko, tlhahlobo ea taxonomic. ea mefuta, tlhahlobo ea ntlafatso ea ts'ebetso ea liphatsa tsa lefutso tse hlahisitsoeng ka mokhoa o fapaneng, le tse ling.
Sethala: Illumina NovaSeq Platform
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Genome ea fungal
Biomarker Technologies e fana ka tlhahlobo ea genome, genome e ntle le genome e felletseng ea fungal ho latela sepheo se itseng sa lipatlisiso.Tatelano ea liphatsa tsa lefutso, kopano le tlhaloso e sebetsang e ka finyelloa ka ho kopanya tatellano ea moloko o latelang + Tatelano ea moloko oa boraro ho finyella kopano ea boemo bo phahameng ba genome.Theknoloji ea Hi-C e ka boela ea sebelisoa ho tsamaisa kopano ea genome boemong ba chromosome.
Sethala:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Lisebelisoa tsa lisebelisoa
BMKCloud ke sethala se etelletseng pele sa bioinformatic se fanang ka tharollo ea sebaka se le seng bakeng sa mananeo a genomic, a tšeptjoang haholo ke bafuputsi mabaleng a fapaneng a kenyeletsang bongaka, temo, tikoloho, joalo-joalo. BMKCloud e na le lisebelisoa tse fapaneng tse sebelisoang khafetsa tsa bioinformatic tse kenyelletsang tlhaloso ea liphatsa tsa lefutso, lisebelisoa tsa liphatsa tsa lefutso, ncRNA, taolo ea boleng ba data, kopano, ho tsamaisana, ho ntšoa ha data, liphetoho, lipalo, jenereithara ea litšoantšo, tatellano. tlhahlobo, joalo-joalo.
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Genome e Felletseng Libaktheria
Biomarker Technologies e fana ka ts'ebeletso ea tatellano mabapi le ho aha genome e felletseng ea libaktheria tse nang le lekhalo le zero.Ts'ebetso ea mantlha ea libaktheria kaho e felletseng ea liphatsa tsa lefutso e kenyelletsa tatellano ea moloko oa boraro, kopano, tlhaloso e sebetsang le tlhahlobo e tsoetseng pele ea bioinformatics e phethahatsang lipheo tse khethehileng tsa lipatlisiso.Profil e felletseng ea genome ea baktheria e fana ka matla a ho senola mekhoa ea mantlha ea ts'ebetso ea bona ea tlhaho, e ka fanang ka leseli la bohlokoa bakeng sa lipatlisiso tsa genomic mefuteng e phahameng ea eukaryotic.
Sethala:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
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RNA e nyane
Li-RNA tse nyane ke mofuta oa RNA e khuts'oane e sa keneng likhoutu ka bolelele ba 18-30 nt, ho kenyeletsoa miRNA, siRNA le piRNA.Li-RNA tsena tse nyenyane li 'nile tsa tlalehoa ka bongata hore li ameha mekhoeng e fapaneng ea likokoana-hloko tse kang ho senyeha ha mRNA, ho thibela phetolelo, ho thehoa ha heterochromatin, joalo-joalo. Tlhahlobo ea tatellano ea SmallRNA e sebelisitsoe haholo lithutong tsa nts'etsopele ea liphoofolo / limela, maloetse, kokoana-hloko, joalo-joalo. Sequencing analysis platform e na le tlhahlobo e tloaelehileng le merafo e tsoetseng pele ea data.Motheong oa data ea RNA-seq, tlhahlobo e tloaelehileng e ka fihlela boitsebahatso le bonohe ba miRNA, ponelopele ea liphatsa tsa lefutso tsa miRNA, tlhaloso le tlhahlobo ea polelo.Tlhahlobo e tsoetseng pele e nolofalletsa ho batla le ho ntšoa ha miRNA, tlhahiso ea setšoantšo sa Venn, miRNA le mohaho oa marang-rang oa liphatsa tsa lefutso.
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NGS-WGS (Illumina/BGI)
NGS-WGS ke sethala se felletseng sa tlhahlobo ea tatellano ea liphatsa tsa lefutso, se ntlafalitsoeng motheong oa boiphihlelo bo bongata ho Biomarker Technologies.Sethala sena se bonolo sa ho se sebelisa se lumella ho fana ka potlako ha ts'ebetso e kopaneng ea tlhahlobo ka ho beha lintlha tse 'maloa tsa motheo, tse lumellanang le data ea tatellano ea DNA e hlahisitsoeng sethaleng sa Illumina le sethala sa tatellano sa BGI.Sethala sena se sebelisoa ho seva sa komporo ea ts'ebetso e phahameng, e matlafatsang tlhahlobo ea data e sebetsang hantle haholo ka nako e lekanyelitsoeng haholo.Merafo ea data ea motho ka mong e fumaneha ho latela tlhahlobo e tloaelehileng, ho kenyeletsoa potso ea mofuta o fetotsoeng, moralo oa mantlha oa PCR, jj.
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mRNA(Reference)
Transcriptome ke sehokelo lipakeng tsa tlhaiso-leseling ea liphatsa tsa lefutso le proteome ea ts'ebetso ea baeloji.Taolo ea boemo ba ho ngoloa ke mokhoa oa bohlokoa ka ho fetisisa le o ithutoang ka ho fetisisa oa taolo ea lintho tse phelang.Transcriptome sequencing e ka tatellano ea transcriptome ka nako leha e le efe ka nako kapa tlas'a boemo leha e le bofe, ka qeto e nepahetseng ho nucleotide e le 'ngoe. E ka bonahatsa ka mokhoa o ts'oanelang boemo ba liphatsa tsa lefutso, ka nako e le 'ngoe e khetholla le ho lekanya litlaleho tse sa tloaelehang le tse tloaelehileng,' me e fane ka boitsebiso ba sebopeho sa mohlala oa litšoantšo tse khethehileng.
Hajoale, thekenoloji ea tatellano ea li-transcriptome e sebelisitsoe haholo ho agronomy, bongaka le mafapheng a mang a lipatlisiso, ho kenyeletsoa taolo ea nts'etsopele ea liphoofolo le limela, ho ikamahanya le maemo a tikoloho, tšebelisano ea 'mele ea ho itšireletsa mafung, tikoloho ea liphatsa tsa lefutso, ho iphetola ha liphatsa tsa lefutso le hlahala le ho lemoha lefu la lefutso.
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Metagenomics (NGS)
Sethala sena sa tlhahlobo se etselitsoe tlhahlobo ea data ea shotgun metagenomic motheong oa boiphihlelo ba lilemo.E na le ts'ebetso e kopantsoeng ea ts'ebetso e nang le litlhahlobo tse fapaneng tsa metagenomics tse hlokahalang hangata ho kenyelletsa ts'ebetso ea data, lithuto tsa boemo ba mefuta, lithuto tsa boemo ba liphatsa tsa lefutso, binning ea metagenome, joalo-joalo. Ho feta moo, lisebelisoa tsa morafo tsa data tse hlophisitsoeng li fumaneha holim'a tlhahlobo e tloaelehileng ea mosebetsi, ho kenyeletsoa potso ea mofuta le mefuta. , tlhophiso ea paramethara, tlhahiso ea motho ka mong, joalo-joalo.
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LncRNA
Li-RNA tse telele tse sa khouteng (lncRNA) ke mofuta oa lingoliloeng tse nang le bolelele bo fetang 200 nt, tse sa khoneng ho khouta liprotheine.Bopaki bo ipokeletseng bo fana ka maikutlo a hore boholo ba li-lncRNA li na le monyetla oa ho sebetsa.Litheknoloji tsa tatellano ea maemo a holimo le lisebelisoa tsa tlhahlobo ea bioinformatic li re fa matla a ho senola tatelano ea lncRNA le ho beha tlhahisoleseling ka katleho le ho re lebisa ho sibolleng li-lncRNA tse nang le mesebetsi ea bohlokoa ea taolo.BMKCloud e motlotlo ho fa bareki ba rona sethala sa tlhahlobo ea lncRNA ho fihlela tlhahlobo e potlakileng, e tšepahalang le e bonolo ea lncRNA.
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GWASE
Boithuto ba Genome-wide Association (GWAS) bo ikemiselitse ho khetholla mefuta e fapaneng ea liphatsa tsa lefutso (genotype) e amanang le litšobotsi tse itseng (phenotype).Lithuto tsa GWA li etsa lipatlisiso tsa matšoao a liphatsa tsa lefutso tse tšelang palo e kholo ea batho ka bomong le ho bolela esale pele mekhatlo ea genotype-phenotype ka tlhahlobo ea lipalo-palo boemong ba baahi.Whole-genome resequencing e ka khona ho sibolla mefuta eohle ea liphatsa tsa lefutso.Ho kopantsoe le lintlha tsa phenotypic, GWAS e ka sebetsoa ho khetholla li-SNP tse amanang le phenotype, li-QTL le liphatsa tsa lefutso tsa likhetho, tse tšehetsang ka matla ho ikatisa ha liphoofolo / limela tsa sejoale-joale.SLAF ke leano le nolofalitsoeng la tatellano ea liphatsa tsa lefutso, le sibollang matšoao a pharalletseng a pharalletseng a genome, SNP.Li-SNP tsena, e le matšoao a liphatsa tsa lefutso tsa molek'hule, li ka sebetsoa bakeng sa lithuto tsa kopanelo tse nang le litšobotsi tse lebisitsoeng.Ke leano le bolokang chelete e ngata ho tsebahatsa litšobotsi tse rarahaneng tse amanang le liphatsa tsa lefutso.
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Nanopore Tlhōlo e feletseng ea transcriptomics
Li-isoform tse rarahaneng le tse feto-fetohang linthong tse phelang ke mekhoa ea bohlokoa ea liphatsa tsa lefutso bakeng sa ho laola liphatsa tsa lefutso le ho fapana ha liprotheine.Khethollo e nepahetseng ea meaho ea mongolo ke motheo oa boithuto bo tebileng ba mekhoa ea taolo ea polelo ea lefutso.Sethala sa tatellano sa Nanopore se atlehile ho tlisa boithuto ba transcriptomic boemong ba isoform.Sethala sena sa tlhahlobo se etselitsoe ho sekaseka data ea RNA-Seq e hlahisitsoeng sethaleng sa Nanopore motheong oa genome ea referense, e fihlelang tlhahlobo ea boleng le palo maemong a liphatsa tsa lefutso le boemo ba lingoliloeng.
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selikalikoe-RNA
CircRNA (circRNA) ke mofuta oa RNA e sa ngolisoeng, e sa tsoa fumanoa e phetha karolo ea bohlokoa ho marang-rang a taolo a amehang ho nts'etsopele, ho hanyetsa tikoloho, joalo-joalo E fapane le limolek'hule tsa RNA tse nang le mela, mohlala, mRNA, lncRNA, 3′ le 5′. lipheletso tsa circRNA li kopantsoe hammoho ho etsa sebopeho se chitja, se li pholosang ho tsoa tšilong ea exonuclease mme li tsitsitse ho feta boholo ba RNA e melang.CircRNA e fumanoe e na le mesebetsi e fapaneng ea ho laola polelo ea lefutso.CircRNA e ka sebetsa joalo ka ceRNA, e tlamang miRNA ka tlholisano, e tsejoang ka seponche sa miRNA.Sethala sa tlhahlobo ea tatellano ea CircRNA se matlafatsa sebopeho sa circRNA le tlhahlobo ea polelo, ponelopele ea sepheo le tlhahlobo e kopaneng le mefuta e meng ea limolek'hule tsa RNA.
