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Sequencing ea Chromatin Immunoprecipitation (ChIP-seq)
ChIP-Seq e fana ka profil ea genome-wide ea lipheo tsa DNA bakeng sa phetoho ea histone, lintlha tsa transcription, le liprotheine tse ling tse amanang le DNA.E kopanya khetho ea chromatin immuno-precipitation (ChIP) ho khutlisa protheine-DNA complexes, ka matla a tatellano ea moloko o latelang (NGS) bakeng sa tatellano e phahameng ea DNA e fumanoeng.Ho feta moo, kaha liprotheine-DNA complexes li fumanoa liseleng tse phelang, libaka tse tlamang li ka bapisoa ka mefuta e fapaneng ea lisele le lisele, kapa tlas'a maemo a sa tšoaneng.Likopo li fapana ho tloha taolong ea mongolo ho ea ho litsela tsa nts'etsopele ho isa mekhoeng ea mafu le ho feta.
Sethala: Illumina NovaSeq Platform
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Metagenomic Sequencing -NGS
Metagenome e bolela pokello ea kakaretso ea liphatsa tsa lefutso tsa sehlopha se tsoakaneng sa lintho tse phelang, tse kang metagenome ea tikoloho, metagenome ea batho, joalo-joalo. E na le liphatsa tsa lefutso tsa likokoana-hloko tse lengoang le tse ke keng tsa lengoa.Metagenomic sequencing ke sesebelisoa sa limolek'hule se sebelisetsoang ho hlahloba lisebelisoa tse tsoakiloeng tsa genomic tse ntšitsoeng lisampoleng tsa tikoloho, tse fanang ka boitsebiso bo qaqileng ka mefuta-futa ea mefuta le bongata, sebopeho sa baahi, kamano ea phylogenetic, liphatsa tsa lefutso tse sebetsang le marang-rang a amanang le maemo a tikoloho.
Sethala:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics ke sesebelisoa sa limolek'hule se sebelisetsoang ho hlahloba lisebelisoa tse tsoakiloeng tsa genomic tse ntšitsoeng lisampoleng tsa tikoloho, tse fanang ka boitsebiso bo qaqileng ka mefuta-futa ea mefuta-futa le bongata, sebopeho sa baahi, kamano ea phylogenetic, liphatsa tsa lefutso tse sebetsang le marang-rang a ho hokahanya le maemo a tikoloho, joalo-joalo, "Nanopore sequencing platforms" e sa tsoa hlahisoa. ho ea lithutong tsa metagenomic.Ts'ebetso ea eona e ikhethang ea bolelele ba ho bala e ntlafalitse haholo tlhahlobo ea metagenomic, haholo kopano ea metagenome.Ho nka melemo ea bolelele ba ho bala, boithuto ba metagenomic bo thehiloeng ho Nanopore bo khona ho fihlela kopano e tsoelang pele ha e bapisoa le metagenomics ea sethunya.Ho phatlalalitsoe hore metagenomics e thehiloeng ho Nanopore e atlehile ho hlahisa liphatsa tsa lefutso tsa baktheria tse felletseng le tse koetsoeng ho tsoa ho li-microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Sethala:Nanopore PromethION P48
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Tatelano e felletseng ea genome bisulfite
DNA methylation sebakeng sa bohlano ho cytosine (5-mC) e na le tšusumetso ea mantlha ho polelo ea liphatsa tsa lefutso le tšebetso ea lisele.Mekhoa e sa tloaelehang ea methylation e 'nile ea amahanngoa le maemo le maloetse a' maloa, a kang kankere.WGBS e fetohile tekanyetso ea khauta ea ho ithuta genome-wide methylation ka qeto ea motheo e le 'ngoe.
Sethala: Illumina NovaSeq Platform
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Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
ATAC-seq ke mokhoa oa tatellano ea maemo a holimo bakeng sa tlhahlobo ea phihlello ea genome-wide chromatin, e leng bohlokoa bakeng sa taolo ea lefats'e ea epigenetic ea polelo ea liphatsa tsa lefutso.Li-adapter tsa tatellano li kenngoa libakeng tse bulehileng tsa chromatin ka hyperactive Tn5 transposase.Kamora ho holisoa ha PCR, ho etsoa laeborari ea tatellano.Libaka tsohle tse bulehileng tsa chromatin li ka fumanoa tlas'a boemo bo itseng ba nako ea sebaka, eseng feela libakeng tse tlamang tsa transcription factor, kapa sebaka se itseng se fetotsoeng sa histone.
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16S/18S/ITS Amplicon Sequencing-PacBio
Subunit e ho 16S le 18S rRNA e nang le libaka tse bolokiloeng haholo le tse feto-fetohang haholo ke monwana o phethahetseng oa limolek'hule bakeng sa boitsebahatso ba likokoana-hloko tsa prokaryotic le eukaryotic.Ho nka monyetla oa tatellano, li-amplicon tsena li ka shebisisoa ho latela likarolo tse bolokiloeng mme libaka tse feto-fetohang li ka tsebahala ka botlalo bakeng sa boitsebahatso ba likokoana-hloko tse kenyang letsoho lithutong tse akaretsang tlhahlobo ea mefuta-futa ea likokoana-hloko, taxonomy, phylogeny, joalo-joalo. ) tatellano ea sethala sa PacBio se nolofalletsa ho fumana lipalo tse telele tse nepahetseng haholo, tse ka koahelang li-amplicon tsa bolelele bo felletseng (hoo e ka bang 1.5 KB).Pono e pharalletseng ea sebaka sa liphatsa tsa lefutso e matlafalitse haholo tharollo ea tlhaloso ea mefuta ea libaktheria kapa li-fungus.
Sethala:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
Tatelano ea amplicon ea 16S/18S/ITS e ikemiselitse ho senola phylogeny, taxonomy, le mefuta e mengata sechabeng sa likokoana-hloko ka ho batlisisa lihlahisoa tsa PCR tsa liphatsa tsa lefutso tsa tlhokomelo ea ntlo tse nang le likarolo tse peli tse buuoang haholo le tse sa fetoheng.Kenyelletso ea likhatiso tsena tsa menoana ea limolek'hule tse phethahetseng ka Woeses et al, (1977) e matlafatsa profiling ea microbiome e se nang ho itšehla thajana.Tatellano ea 16S (libaktheria), 18S (fungus) le Internal transcribed spacer(ITS, fungus) e lumella ho tsebahatsa mefuta e mengata e mengata hammoho le mefuta e sa tloaelehang le e sa tsejoeng.Theknoloji ena e se e le sesebelisoa se sebelisoang haholo le se seholo ho khetholla sebopeho sa likokoana-hloko tse fapaneng libakeng tse fapaneng, joalo ka molomo oa motho, mala, mantle, joalo-joalo.
Sethala:Illumina NovaSeq Platform
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Libaktheria le Fungus Whole Genome Re-Sequencing
Tatelano bocha ea genome ea baktheria le fungal ke sesebelisoa sa bohlokoa sa ho phethela liphatsa tsa lefutso tse tsejoang tsa baktheria le li-fungus, hammoho le ho bapisa liphatsa tsa lefutso tse ngata kapa ho etsa 'mapa oa liphatsa tsa lefutso tse ncha.Ho bohlokoa haholo ho tatellano ea liphatsa tsa lefutso tsa baktheria le li-fungus e le ho hlahisa liphatsa tsa lefutso tse nepahetseng, ho etsa boitsebahatso ba likokoana-hloko le lithuto tse ling tsa papiso ea genome.
Sethala: Illumina NovaSeq Platform
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PacBio-Full-length 16S/18S/ITS Amplicon Sequencing
Sethala sa Amplicon (16S/18S/ITS) se entsoe ka boiphihlelo ba lilemo tsa tlhahlobo ea projeke ea mefuta-futa ea likokoana-hloko, e nang le tlhahlobo e tloaelehileng ea mantlha le tlhahlobo ea motho ka mong: tlhahlobo ea mantlha e akaretsa litaba tsa tlhahlobo e akaretsang ea lipatlisiso tsa hajoale tsa likokoana-hloko, litaba tsa tlhahlobo li ruile ebile li na le botlalo, le liphetho tsa tlhahlobo li hlahisoa ka mokhoa oa litlaleho tsa morero;Litaba tsa tlhahlobo ea motho ka mong li fapane.Mehlala e ka khethoa 'me liparamente li ka hlophisoa ka mokhoa o bonolo ho latela tlaleho ea tlhahlobo ea mantlha le sepheo sa lipatlisiso, ho fihlela litlhoko tsa motho ka mong.Sistimi ea ts'ebetso ea Windows, e bonolo ebile e potlakile.
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PacBio-Full-length Transcriptome (Non-Reference)
Ho nka data ea tatellano ea Isoform ea Pacific Bioscience (PacBio) joalo ka ho kenya, Sesebelisoa sena se khona ho tsebahatsa tatelano ea sengoloa sa bolelele bo felletseng (ntle le ho kopanya).Ka ho etsa 'mapa oa tatellano ea bolelele bo felletseng khahlano le genome ea litšupiso, lingoliloeng li ka ntlafatsoa ka liphatsa tsa lefutso tse tsebahalang, lingoliloeng, libaka tsa likhoutu, joalo-joalo. Tabeng ena, ho ka finyelloa boitsebiso bo nepahetseng haholoanyane ba meaho ea mRNA, joalo ka mefuta e meng ea ho kopanya, joalo-joalo.Tlhahlobo e kopaneng le data ea tatellano ea li-transcriptome ea NGS e etsa hore ho be le tlhaloso e felletseng le palo e nepahetseng haholoanyane ea polelo maemong a mongolo, e leng molemo haholo oa polelo e fapaneng le tlhahlobo ea ts'ebetso.
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Representation Bisulfite Sequencing (RRBS)
Lipatlisiso tsa DNA methylation esale e le taba e chesang haholo lipatlisisong tsa mafu, 'me e amana haufi-ufi le liphatsa tsa lefutso le litšobotsi tsa pheno-typic.RRBS ke mokhoa o nepahetseng, o sebetsang hantle le oa moruo bakeng sa lipatlisiso tsa DNA methylation.Matlafatso ea libaka tsa lihlekehleke tsa CpG ka enzymatic cleavage (Msp I), e kopantsoeng le tatellano ea Bisulfite, e fana ka tlhahlobo e phahameng ea DNA methylation.
Sethala: Illumina NovaSeq Platform
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Prokaryotic mRNA tatelano
tatellano ea mRNA e matlafatsa profil e felletseng ea lingoliloeng tsohle tsa mRNA ka har'a lisele tlas'a maemo a ikhethileng.Theknoloji ena e tsoetseng pele e sebetsa e le sesebelisoa se matla, se senolang liphatlalatso tse rarahaneng tsa liphatsa tsa lefutso, libopeho tsa liphatsa tsa lefutso, le mekhoa ea limolek'hule e amanang le mekhoa e fapaneng ea tlhaho.E amohetsoe haholo lipatlisisong tsa mantlha, tlhahlobo ea bongaka, le nts'etsopele ea lithethefatsi, tatellano ea mRNA e fana ka leseli mabapi le ho rarahana ha matla a cellular le taolo ea liphatsa tsa lefutso.Ts'ebetso ea rona ea sampole ea prokaryotic mRNA e etselitsoe li-transcriptomes tsa prokaryotic, tse kenyelletsang ho fokotseha ha rRNA le ho hlophisoa ha laeborari ea tataiso.
Sethala: Illumina NovaSeq X
