Li-Spatial transcriptomics li ka pele ho boqapi ba mahlale, li matlafatsa bafuputsi ho batlisisa mekhoa e rarahaneng ea liphatsa tsa lefutso ka har'a lisele ha ba ntse ba boloka maemo a bona a sebaka.Har'a li-platform tse fapaneng, BMKGene e hlahisitse BMKManu S1000 Spatial Transcriptome Chip, e ithorisa katharollo e ntlafalitsoengea 5µM, ho fihla ho subcellular range, le ho nolofalletsalitlhophiso tsa tharollo ea maemo a mangata.Chip ea S1000, e nang le matheba a ka bang limilione tse 2, e sebelisa li-microwell tse koahetsoeng ka lifaha tse nang le lisebelisoa tsa ho hapa tse nang le barcode.Laeborari ea cDNA, e ntlafalitsoeng ka li-barcode tsa sebaka, e lokisoa ho tsoa ho chip ea S1000 mme ka mor'a moo e hlophisoa sethaleng sa Illumina NovaSeq.Motsoako oa disampole tse nang le barcoded tsa sebaka le li-UMI li netefatsa ho nepahala le ho nepahala ha data e hlahisitsoeng.Tšobotsi e ikhethang ea BMKManu S1000 chip e itšetlehile ka ho feto-fetoha ha eona, e fana ka litlhophiso tsa maemo a mangata tse ka hlophisoang hantle ho lisele tse fapaneng le maemo a lintlha.Ho ikamahanya le maemo hona ho beha chip e le khetho e ikhethang bakeng sa lithuto tse fapaneng tsa transcriptomics tsa sebaka, e netefatsang kopano e nepahetseng ea sebaka ka lerata le fokolang.
Ba sebelisa chip ea BMKManu S1000 le mahlale a mang a transcriptomics a sebaka, bafuputsi ba ka fumana kutloisiso e betere ea mokhatlo oa sebaka sa lisele le litšebelisano tse rarahaneng tsa limolek'hule tse etsahalang ka har'a lisele, ho fana ka leseli la bohlokoa mabapi le lits'ebetso tsa ts'ebetso ea bioloji libakeng tse fapaneng, ho kenyeletsoa. oncology, neuroscience, tsoelopele ea baeloji, immunology le lithuto tsa botanical.
Platform: BMKManu S1000 chip le Illumina NovaSeq