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Metagenomic Sequencing -NGS
Metagenome e bolela pokello ea kakaretso ea liphatsa tsa lefutso tsa sehlopha se tsoakaneng sa lintho tse phelang, tse kang metagenome ea tikoloho, metagenome ea batho, joalo-joalo. E na le liphatsa tsa lefutso tsa likokoana-hloko tse lengoang le tse ke keng tsa lengoa.Metagenomic sequencing ke sesebelisoa sa limolek'hule se sebelisetsoang ho hlahloba lisebelisoa tse tsoakiloeng tsa genomic tse ntšitsoeng lisampoleng tsa tikoloho, tse fanang ka boitsebiso bo qaqileng ka mefuta-futa ea mefuta le bongata, sebopeho sa baahi, kamano ea phylogenetic, liphatsa tsa lefutso tse sebetsang le marang-rang a amanang le maemo a tikoloho.
Sethala:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics ke sesebelisoa sa limolek'hule se sebelisetsoang ho hlahloba lisebelisoa tse tsoakiloeng tsa genomic tse ntšitsoeng lisampoleng tsa tikoloho, tse fanang ka boitsebiso bo qaqileng ka mefuta-futa ea mefuta-futa le bongata, sebopeho sa baahi, kamano ea phylogenetic, liphatsa tsa lefutso tse sebetsang le marang-rang a ho hokahanya le maemo a tikoloho, joalo-joalo, "Nanopore sequencing platforms" e sa tsoa hlahisoa. ho ea lithutong tsa metagenomic.Ts'ebetso ea eona e ikhethang ea bolelele ba ho bala e ntlafalitse haholo tlhahlobo ea metagenomic, haholo kopano ea metagenome.Ho nka melemo ea bolelele ba ho bala, boithuto ba metagenomic bo thehiloeng ho Nanopore bo khona ho fihlela kopano e tsoelang pele ha e bapisoa le metagenomics ea sethunya.Ho phatlalalitsoe hore metagenomics e thehiloeng ho Nanopore e atlehile ho hlahisa liphatsa tsa lefutso tsa baktheria tse felletseng le tse koetsoeng ho tsoa ho li-microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Sethala:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Subunit e ho 16S le 18S rRNA e nang le libaka tse bolokiloeng haholo le tse feto-fetohang haholo ke monwana o phethahetseng oa limolek'hule bakeng sa boitsebahatso ba likokoana-hloko tsa prokaryotic le eukaryotic.Ho nka monyetla oa tatellano, li-amplicon tsena li ka shebisisoa ho latela likarolo tse bolokiloeng mme libaka tse feto-fetohang li ka tsebahala ka botlalo bakeng sa boitsebahatso ba likokoana-hloko tse kenyang letsoho lithutong tse akaretsang tlhahlobo ea mefuta-futa ea likokoana-hloko, taxonomy, phylogeny, joalo-joalo. ) tatellano ea sethala sa PacBio se nolofalletsa ho fumana lipalo tse telele tse nepahetseng haholo, tse ka koahelang li-amplicon tsa bolelele bo felletseng (hoo e ka bang 1.5 KB).Pono e pharalletseng ea sebaka sa liphatsa tsa lefutso e matlafalitse haholo tharollo ea tlhaloso ea mefuta ea libaktheria kapa li-fungus.
Sethala:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
Tatelano ea amplicon ea 16S/18S/ITS e ikemiselitse ho senola phylogeny, taxonomy, le mefuta e mengata sechabeng sa likokoana-hloko ka ho batlisisa lihlahisoa tsa PCR tsa liphatsa tsa lefutso tsa tlhokomelo ea ntlo tse nang le likarolo tse peli tse buuoang haholo le tse sa fetoheng.Kenyelletso ea likhatiso tsena tsa menoana ea limolek'hule tse phethahetseng ka Woeses et al, (1977) e matlafatsa profiling ea microbiome e se nang ho itšehla thajana.Tatellano ea 16S (libaktheria), 18S (fungus) le Internal transcribed spacer(ITS, fungus) e lumella ho tsebahatsa mefuta e mengata e mengata hammoho le mefuta e sa tloaelehang le e sa tsejoeng.Theknoloji ena e se e le sesebelisoa se sebelisoang haholo le se seholo ho khetholla sebopeho sa likokoana-hloko tse fapaneng libakeng tse fapaneng, joalo ka molomo oa motho, mala, mantle, joalo-joalo.
Sethala:Illumina NovaSeq Platform
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Libaktheria le Fungus Whole Genome Re-Sequencing
Tatelano bocha ea genome ea baktheria le fungal ke sesebelisoa sa bohlokoa sa ho phethela liphatsa tsa lefutso tse tsejoang tsa baktheria le li-fungus, hammoho le ho bapisa liphatsa tsa lefutso tse ngata kapa ho etsa 'mapa oa liphatsa tsa lefutso tse ncha.Ho bohlokoa haholo ho tatellano ea liphatsa tsa lefutso tsa baktheria le li-fungus e le ho hlahisa liphatsa tsa lefutso tse nepahetseng, ho etsa boitsebahatso ba likokoana-hloko le lithuto tse ling tsa papiso ea genome.
Sethala: Illumina NovaSeq Platform
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Metatranscriptome Sequencing
Metatranscriptome sequencing e supa liphatsa tsa lefutso tsa likokoana-hloko (ka bobeli eukaryotes le prokaryotes) ka har'a tikoloho ea tlhaho (e leng mobu, metsi, leoatle, mantle le mala.) Haholo-holo, litšebeletso tsena li u lumella ho fumana boitsebiso bo feletseng ba liphatsa tsa lefutso tsa lichaba tse rarahaneng tsa likokoana-hloko, tlhahlobo ea taxonomic. ea mefuta, tlhahlobo ea ntlafatso ea ts'ebetso ea liphatsa tsa lefutso tse hlahisitsoeng ka mokhoa o fapaneng, le tse ling.
Sethala: Illumina NovaSeq Platform
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Genome ea fungal
Biomarker Technologies e fana ka tlhahlobo ea genome, genome e ntle le genome e felletseng ea fungal ho latela sepheo se itseng sa lipatlisiso.Tatelano ea liphatsa tsa lefutso, kopano le tlhaloso e sebetsang e ka finyelloa ka ho kopanya tatellano ea moloko o latelang + Tatelano ea moloko oa boraro ho finyella kopano ea boemo bo phahameng ba genome.Theknoloji ea Hi-C e ka boela ea sebelisoa ho tsamaisa kopano ea genome boemong ba chromosome.
Sethala:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Genome e Felletseng Libaktheria
Biomarker Technologies e fana ka ts'ebeletso ea tatellano mabapi le ho aha genome e felletseng ea libaktheria tse nang le lekhalo le zero.Ts'ebetso ea mantlha ea libaktheria kaho e felletseng ea liphatsa tsa lefutso e kenyelletsa tatellano ea moloko oa boraro, kopano, tlhaloso e sebetsang le tlhahlobo e tsoetseng pele ea bioinformatics e phethahatsang lipheo tse khethehileng tsa lipatlisiso.Profil e felletseng ea genome ea baktheria e fana ka matla a ho senola mekhoa ea mantlha ea ts'ebetso ea bona ea tlhaho, e ka fanang ka leseli la bohlokoa bakeng sa lipatlisiso tsa genomic mefuteng e phahameng ea eukaryotic.
Sethala:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
