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Genome-wide Association Analysis
Boithuto ba Genome-wide Association (GWAS) bo ikemiselitse ho khetholla mefuta e fapaneng ea liphatsa tsa lefutso (genotype) e amanang le litšobotsi tse itseng (phenotype).Boithuto ba GWAS bo batlisisa matšoao a liphatsa tsa lefutso tse tšelang palo e kholo ea batho ka bomong le ho bolela esale pele mekhatlo ea genotype-phenotype ka tlhahlobo ea lipalo maemong a baahi.E sebelisitsoe haholo lipatlisisong tsa mafu a batho le merafo e sebetsang ea liphatsa tsa lefutso ka litšobotsi tse rarahaneng tsa liphoofolo kapa limela.
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Tatelano ya Genome ya Semela/Aphoofolo
Whole genome re-sequencing, eo hape e tsejoang e le WGS, e thusa ho senola liphetoho tse tloaelehileng le tse sa tloaelehang ho genome eohle ho kenyelletsa Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), le Copy Number Variation (CNV). ).Li-SV li etsa karolo e kholoanyane ea motheo oa ho fapana ho feta li-SNP 'me li na le tšusumetso e kholo ho genome, e nang le phello e kholo ho lintho tse phelang.Requencing ea nako e telele e lumella ho tsebahatsa ka nepo likhechana tse kholo le liphapang tse rarahaneng hobane ho bala nako e telele ho etsa hore ho be bonolo haholo ho tšela chromosomal libakeng tse rarahaneng joalo ka ho pheta-pheta tandem, libaka tse ruileng tsa GC / AT, le libaka tse feto-fetohang.
Sethala: Illumina, PacBio, Nanopore
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Liphatsa tsa lefutso tsa ho iphetola ha lintho
Evolutionary genetics ke ts'ebeletso e hlophisitsoeng ea tatelano e etselitsoeng ho fana ka tlhaloso e felletseng mabapi le tlhaiso-leseling ea ho iphetola ha lintho ea thepa e fanoeng e ipapisitseng le liphatsa tsa lefutso, ho kenyeletsoa SNPs, InDels, SVs le CNVs.E fana ka tlhahlobo eohle ea motheo e hlokahalang bakeng sa ho hlalosa liphetoho tsa ho iphetola ha lintho le likarolo tsa lefutso tsa baahi, joalo ka sebopeho sa baahi, mefuta-futa ea liphatsa tsa lefutso, likamano tsa phylogeny, joalo-joalo. E boetse e na le lithuto tsa phallo ea liphatsa tsa lefutso, tse matlafatsang khakanyo ea boholo bo sebetsang ba baahi, nako ea ho fapana.
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Papiso ea Genomics
Papiso ea genomics ha e le hantle e bolela ho bapisa tatellano e felletseng ea liphatsa tsa lefutso le libopeho tsa mefuta e fapaneng.Taeo ena e ikemiselitse ho senola phetoho ea mefuta, tšebetso ea liphatsa tsa lefutso, mokhoa oa taolo ea liphatsa tsa lefutso maemong a genome ka ho tsebahatsa libopeho tsa tatelano le likarolo tse bolokileng kapa tse arohanyang mefuta e fapaneng.Boithuto bo tloaelehileng ba ho bapisa liphatsa tsa lefutso bo kenyelletsa tlhahlobo ea liphatsa tsa lefutso, nts'etsopele ea ho iphetola ha lintho, phetiso e felletseng ea genome, khatello ea khetho, jj.
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Hi-C e thehiloeng ho Genome Assembly
Hi-C ke mokhoa o etselitsoeng ho ts'oara tlhophiso ea chromosome ka ho kopanya litšebelisano tse thehiloeng ho proximity le tatellano ea maemo a holimo.Ho lumeloa hore matla a litšebelisano tsena a amana hampe le sebaka sa 'mele ho li-chromosome.Ka hona, lintlha tsa Hi-C li ka tataisa ho kopanya, ho hlophisa le ho tsamaisa tatellano e kopaneng ho genome e hlophisitsoeng le ho tiisa tseo palo e itseng ea li-chromosome.Theknoloji ena e matlafatsa kopano ea liphatsa tsa lefutso tsa boemo ba chromosome ha ho se na 'mapa oa lefutso o thehiloeng ho baahi.Genome e 'ngoe le e' ngoe e hloka Hi-C.
Sethala: Illumina NovaSeq Platform / DNBSEQ
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Semela / Phoofolo De Novo Genome Sequencing
Ho tloha Novotatellano e bolela kaho ea mofuta oohle oa lefutso ho sebelisoa theknoloji ea tatelano, mohlala, PacBio, Nanopore, NGS, joalo-joalo, ha ho se na genome.Ntlafatso e tsotehang ea bolelele ba ho bala ba theknoloji ea tatellano ea moloko oa boraro e tlisitse menyetla e mecha ea ho bokella liphatsa tsa lefutso tse rarahaneng, tse kang tse nang le heterozygosity e phahameng, karo-karolelano e phahameng ea libaka tse pheta-phetoang, li-polyploids, joalo-joalo. ho rarolla lintlha tse ipheta-phetang, libaka tse nang le litaba tsa GC tse sa tloaelehang le libaka tse ling tse rarahaneng haholo.
Sethala: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq Platform
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Sequencing ea Exome ea Motho e Felletseng
Whole exome sequencing (WES) e nkuoa e le leano la tatelano le baballang chelete bakeng sa ho tseba liphetoho tse bakang mafu.Leha li-exons li nka hoo e ka bang 1.7% ea genome kaofela, e emela boemo ba tšebetso ea protheine ka kotloloho.Liphatseng tsa lefutso tsa motho, ho tlalehiloe hore ho feta 85% ea liphetoho tse amanang le mafu li etsahala sebakeng sa protheine ea likhoutu.
BMKGENE e fana ka lits'ebeletso tse felletseng le tse tenyetsehang tsa tatellano ea batho ka kakaretso ka maano a fapaneng a ho hapa a fumanehang ho fihlela lipheo tse fapaneng tsa lipatlisiso.
Sethala: Illumina NovaSeq Platform
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, haholo-holo ho baahi ba bangata, ke mohato oa bohlokoa lithutong tsa liphatsa tsa lefutso, tse fanang ka motheo oa liphatsa tsa lefutso bakeng sa ho sibolloa ha liphatsa tsa lefutso, tlhahlobo ea ho iphetola ha lintho, joalo-joalo Ho e-na le tatellano e tebileng ea liphatsa tsa lefutso, ho fokotseha ha tatellano ea liphatsa tsa lefutso (RRGS). ) e hlahisoa ho fokotsa litšenyehelo tsa tatellano ea sampuli ka 'ngoe, ha ho ntse ho boloka ts'ebetso e utloahalang ho sibolloeng ha lets'oao la lefutso.Hangata sena se finyelloa ka ho ntša sekhechana sa lithibelo ka har'a boholo bo fanoeng, se bitsoang laeborari ea boemeli bo fokolitsoeng (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) ke leano le iketselitseng la SNP genotyping ka kapa ntle le genome ea referense.
Sethala: Illumina NovaSeq Platform
