Lihlahisoa

Tatelano ya Genome ya Semela/Aphoofolo

Whole genome re-sequencing, eo hape e tsejoang e le WGS, e thusa ho senola liphetoho tse tloaelehileng le tse sa tloaelehang ho genome eohle ho kenyelletsa Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), le Copy Number Variation (CNV). ).Li-SV li etsa karolo e kholoanyane ea motheo oa ho fapana ho feta li-SNP 'me li na le tšusumetso e kholo ho genome, e nang le phello e kholo ho lintho tse phelang.Requencing ea nako e telele e lumella ho tsebahatsa ka nepo likhechana tse kholo le liphapang tse rarahaneng hobane ho bala nako e telele ho etsa hore ho be bonolo haholo ho tšela chromosomal libakeng tse rarahaneng joalo ka ho pheta-pheta tandem, libaka tse ruileng tsa GC / AT, le libaka tse feto-fetohang.

Sethala: Illumina, PacBio, Nanopore

Iteanye le rona

Lintlha tsa Tšebeletso

Sephetho sa Demo

Phatlalatso e Featured

1.Melemo ea Ts'ebeletso

Boiphihlelo bo pharalletseng ba tatellano ea liphatsa tsa lefutso bakeng sa mefuta e fetang 1000.

Linyeoe tse fetang 800 tse phatlalalitsoeng tse nang le tšusumetso e fetang 4000.

Tlhahlobo e felletseng ea bioinformatics mabapi le mehala e fapaneng le tlhahlobo ea ts'ebetso.

2. Litlhaloso tsa Tšebeletso

Sethala		Laebrari	Botebo ba seq bo khothalelitsoeng
Illumina		PE150	Bakeng sa SNP, InDel e letsa ≥ 10x Bakeng sa SV, CNY e letsetsa ≥ 30x
Nanopore		8kb	Bakeng sa SV, CNY e letsetsa ≥ 20x
Pacbio	CCS	15 khb	Bakeng sa SNP, InDel, SV, CNY e letsetsa ≥ 10x

3. Litlhoko tsa Mohlala

Sethala		Conc.(ng/μL)	Chelete (ng)	Bohloeki		Gel ea agarose
				OD260/280	OD260/230	1. Hlakola sehlopha se seholo se se nang letho kapa se lekanyelitsoengho senyeha ho hlokometsoeng ka gel. 2. Ha ho na RNA kapa tšilafalo e fokolang ea protheine
Illumina		≥1	≥30	-	-
Nanopore		≥30	E ipapisitse le tlhahiso ea data 10μg/sele	1.7-2.2	≥1.5
Pacbio	CCS	≥50		1.7-2.2	1.8-2,5

4. Tlhahlobo ea Bioinformation

5. Phallo ea Mosebetsi oa Tšebeletso

Mohlala oa thomello

Ho ntšoa ha DNA

Kaho ea laebrari

Tatelano

Tlhahlobo ea lintlha

Phano ea lintlha

E fetileng: BMKMANU S1000 Spatial Transcriptome

E 'ngoe: Sequloana se le seng sa RNA Sequencing

1) Lipalopalo tsa Genome Mapping

Lethathamo la 1 Lipalopalo tsa sephetho sa 'mapa

Setšoantšo sa 1 Kabo ea boholo ba ho kenya le ho bala sekoahelo.

2) Phapang ea ho fumanoa

Setšoantšo sa 2 Lipalopalo le tlhaloso ea SNP/INDEL/SV har'a lisampole

Setšoantšo sa 3 Kabo ea liphatsa tsa lefutso ka bophara

3) Tlhaloso e sebetsang ea mefuta e fapaneng

2019	Lipuisano tsa tlhaho	Requencing ea lefutso lohle e senola tšimoloho ea Brassica napus le liphatsa tsa lefutso tse amehang ntlafatsong ea eona
2020	PNAS	Tšimoloho ea ho iphetola ha lintho le nalane ea lehae ea tlhapi ea khauta (Carassius auratus)
2021	Plant Biotechnology Journal	Liphatsa tsa lefutso tsa mefuta e 'meli e lenngoeng ea jute
2021	Plant Biotechnology Journal	Lisaeno tsa genomic tsa allopolyploid ea peo ea meroho le oli ea Brassicajuncea le liphatsa tsa lefutso tse laolang ho bokellana ha glucosinolate.
2019	Semela sa Molek'hule	Requencing ea liphatsa tsa lefutso kaofela tsa pokello ea lefats'e ka bophara ea lipese tsa liphoofolo tse rutoang li senola motheo oa liphatsa tsa lefutso tsa phapang ea bona ea ecotype.
2022	Lipatlisiso tsa Temo ea Lirapa	Tlhahlobo ea Genome-wide Association e fana ka leseli la limolek'hule mabapi le phapang ea tlhaho ea boholo ba peo ea lehapu
2021	Journal of Testing Botany	Boithuto ba mokhatlo ka bophara ba Genome bo supa mefuta e fapaneng ea GhSAD1 e fanang ka mamello e batang ka k'hothone.
2021	Journal of Testing Botany	Boithuto ba mokhatlo oa Genome-wide le papiso ea transcriptome e senola buka ea QTL le liphatsa tsa lefutso tse laolang boholo ba petal ho peteed.