PHEPO PHETOHO
Genome De novo
Kopano| Boikemisetso ba thobalano Mesebetsi eohle ea tatellano le lits'ebeletso tse sa fellang tsa bioinformatic li fanoe ke Biomarker Techonologies.
Kakaretso
“Sebōpeho se ikhethang sa li-seadragons se akarelletsa likaroloana tse kang makhasi, molomo o se nang meno le ho ima ha banna ho kopanyelletsang ho fuama mahe a emolisitsoeng “lephatleng” le bulehileng.We de novo–sequenced male and female genomes of common seadragon (Phyllopteryx taeniolatus) le mefuta ea eona e amanang haufi-ufi, alligator pipefish (Syngnathoides biaculeatus).Litlaleho tse ngotsoeng ka mokhoa oa ho iphetola ha lintho, li-appendages tse kang makhasi, li bonts'a hore mefuta e mengata ea liphatsa tsa lefutso e amehang ntlafatsong ea ho qetela e khethiloe hammoho le ntlafatso ea lingoliloeng bakeng sa tokiso ea lisele le liphatsa tsa lefutso tsa tšireletso ea 'mele.Li-zebrafish mutants bakeng sa scpp5, tse lahlehileng ho li-sygnathids tsohle, li fumanoe li haelloa kapa li na le meno a holofetseng a pharyngeal, a tšehetsa khopolo ea hore tahlehelo ea scpp5 e tlatselitse ho lahleheloa ke meno ka syngnathids.Ho ile ha hloauoa sebaka se khethollang thobalano e kenyelletsang mofuta oa amhr2y oa banna o arolelanoang ke common seadragon le alligator pipefish. ”
Lisebelisoa le mekhoa:
Lisebelisoa
FSeadragon ea rona e tloaelehileng (P. taeniolatus) le alligator pipefish (S. biaculeatus) mehlala.Li-seadragon tse peli tse tloaelehileng (e le 'ngoe e motona le e tšehali) le li-pipefish tse peli (e le 'ngoe e tona le e tšehali) li ile tsa sebelisoa bakeng sa tatellano ea liphatsa tsa lefutso,' me banna ba bang ba babeli ba tloaelehileng ba seadragon ba ile ba sebelisoa bakeng sa ho etsa liphatsa tsa lefutso.
Leano la Genome Sequencing
270/350 bp library (Hiseq 2500) + Pacbio 20Kb (Sequel) kapa Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000).sequencing, le batho ba bang ba babeli ba tloaelehileng ba seadragon batho ka bomong ba ile ba sebelisoa bakeng sa genome resequencing.
Sequencing ea Transcriptome
Fkapa seadragon e tloaelehileng, boko, leihlo, gill, sebete, pelo, liphio, mesentery, mala, mesifa, mapheoana, letlalo, likaroloana tse kang makhasi, testis, le ovary.
Leano la kopano ea Genome de novo
Canu (Tokiso) + WTDBG2 (Assembly) + Pilon(Polish) + Lachesis (Hi-C).
Liphetho tse ka Sehloohong
Setšoantšo sa 1. Likarolo tsa bohlokoa tsa seadragon e tloaelehileng (P. taeniolatus) le alligator pipefish (S. biaculeatus) le maemo a tsona a phylogenetic.
Setšoantšo sa 2. Morphology le liphatsa tsa lefutso tsa li-appendages tse kang makhasi ho seadragon e tloaelehileng (P. taeniolatus).
Setšoantšo sa 3. Sefutho se ikemiselitseng ho kopanela liphate ho common seadragon (P. taeniolatus) le alligator pipefish (S. biaculeatus).
Setšoantšo sa 4. Pharyngeal meno phenotypes ho zebrafish scpp5 homozygous mutants.
Nako ea poso: Jan-08-2022