PHETOHO EA LITŠOANTŠISO, PANGENOME
Pan-genome ke eng?
Bopaki bo bokelletsoeng bo bontša hore phapang pakeng tsa mefuta e sa tšoaneng ea mefuta e ka ba khōlō.Genome e le 'ngoe ha e lekane ho fumana boitsebiso bohle ba liphatsa tsa lefutso tsa mofuta o le mong.Sepheo sa thuto ea Pan-genome ke ho fumana setšoantšo se pharaletseng sa mofuta oa mofuta le ho khetholla likamano pakeng tsa litšobotsi le liphatsa tsa lefutso ka ho etsa kopano ea genome de novo ea mefuta e mengata, e lumellang hore ho be le merafo e tebileng le e pharaletseng ea mefuta-futa.
Mekhoa ea Thuto ea Pan-genome
Setšoantšo sa 1 Mekhoa ea lipampiri tsa lipatlisiso tse hatisitsoeng tsa Pan-genome.
Tlhokomeliso: Palo e bonts'a sephetho sa ho nka "pan-genome" e le lentsoe la bohlokoa ho batla lihlooho tsa lingoliloeng tse hatisitsoeng likoranteng tsa letoto la Nature, Cell le Science.
a.Lipalo ho tsoa ho disampole tse ngata li hokahantsoe le litšupiso mme tse sa lumellaneng li bokelloa ho li-contigs tse ncha.Ka ho kenyelletsa li-contigs tsena tsa lipale ho tatellano ea litšupiso tsa mantlha, ho ka etsoa tšupiso ea pangenome.Libaka tse ka sebelisoang li khethoa ho ipapisitsoe le 'mapa oa limmapa tsohle ho khutlela ho pangenome.
b.Kopano ea De novo ea liphatsa tsa lefutso tsa li-accessions tse ngata e lumella mekhoa eohle ea ho hokahanya ha liphatsa tsa lefutso ho khetholla libaka tsa genomic tse ka sebelisoang.
c.Kerafo ea pan-genome e ka hahuoa ho tsoa ho lialgments tsa genome kapa ka kopano ea graph ea de novo, e bolokang tlhaiso-leseling e fapaneng ea libaka tse ka sebelisoang e le litsela tse ikhethang ho graph.
Mokhoa oa ho etsa Pan-genome?
Setšoantšo sa 2 Papiso ea mekhoa ea pan-genome1
a.Lipalo ho tsoa ho disampole tse ngata li hokahantsoe le litšupiso mme tse sa lumellaneng li bokelloa ho li-contigs tse ncha.Ka ho kenyelletsa li-contigs tsena tsa lipale ho tatellano ea litšupiso tsa mantlha, ho ka etsoa tšupiso ea pangenome.Libaka tse ka sebelisoang li khethoa ho ipapisitsoe le 'mapa oa limmapa tsohle ho khutlela ho pangenome.
b.Kopano ea De novo ea liphatsa tsa lefutso tsa li-accessions tse ngata e lumella mekhoa eohle ea ho hokahanya ha liphatsa tsa lefutso ho khetholla libaka tsa genomic tse ka sebelisoang.
c.Kerafo ea pan-genome e ka hahuoa ho tsoa ho lialgments tsa genome kapa ka kopano ea graph ea de novo, e bolokang tlhaiso-leseling e fapaneng ea libaka tse ka sebelisoang e le litsela tse ikhethang ho graph.
Haufinyane tjena Pan-genomes
● Rape pan-genomes2
● Li-pan-genome tsa tamati 3
● Rice Pan -genome4
● Soneblomo Pan-genome5
● Soybean Pan-genome 6
● Rice Pan-genome7
● Barley Pan-genome8
● Koro Pan-genome9
● Mabele Pan-genome10
● Phytoplankton Pan-genome11
Referense
1. Bayer PE, Golicz AA, Scheben A, Batley J, Edwards D. Plant pan-genomes ke boitsebiso bo bocha.Nat Plants.2020;6(8):914-920.doi:10.1038/s41477-020-0733-0
2. Pina JM, Guan Z, Hu J, et al.Liphatsa tsa lefutso tse robeli tsa boleng bo holimo li senola meralo ea pan-genome le phapang ea ecotype ea Brassica napus.Nat Plants.2020;6(1):34-45.doi:10.1038/s41477-019-0577-7
3. Gao L, Gonda I, Sun H, le ba bang.Tomate pan-genome e senola mefuta e mecha le tatso ea litholoana tse sa tloaelehang.Nat Genet.2019;51(6):1044-1051.doi:10.1038/s41588-019-0410-2
4. Zhao Q, Feng Q, Lu H, et al.Tlhahlobo ea Pan-genome e totobatsa boholo ba phapang ea genomic raese e lengoang le e hlaha [tokiso e hatisitsoeng e hlaha ho Nat Genet.2018 Aug;50(8):1196].Nat Genet.2018;50(2):278-284.doi:10.1038/s41588-018-0041-z
5. Hübner S, Bercovich N, Todesco M, et al.Tlhahlobo ea sonobolomo ea liphatsa tsa lefutso e bontša hore hybridization e fetotse liphatsa tsa lefutso le ho hanyetsa mafu.Nat Plants.2019;5(1):54-62.doi:10.1038/s41477-018-0329-0
6. Liu Y, Du H, Li P, et al.Pan-Genome ea Li-soya tse Hlaha le tse Lenngoeng.Sele.2020;182(1):162-176.e13.doi:10.1016/j.cell.2020.05.023
7. Qin P, Lu H, Du H, et al.Tlhahlobo ea Pan-genome ea liphatsa tsa lefutso tse 33 tse fapaneng tsa raese e senola mefuta e patiloeng ea genomic [e phatlalalitsoeng inthaneteng pele ho khatiso, 2021 May 25].Sele.2021;S0092-8674(21)00581-X.doi:10.1016/j.cell.2021.04.046
8. Jayakodi M, Padmarasu S, Haberer G, et al.Pan-genome ea harese e senola lefa le ipatileng la ho ikatisa.Tlhaho.2020;588(7837):284-289.doi:10.1038/s41586-020-2947-8
9. Walkowiak S, Gao L, Monat C, le ba bang.Liphatsa tsa lefutso tse ngata tsa koro li senola phapang ea lefats'e ho tsoalisong ea sejoale-joale.Tlhaho.2020;588(7837):277-283.doi:10.1038/s41586-020-2961-x
10. Tao Y, Luo H, Xu J, et al.Phapang e batsi ka har'a pan-genome ea mabele a lengoang le a hlaha [e phatlalalitsoe inthaneteng pele e hatisoa, 2021 May 20].Nat Plants.2021;10.1038 / s41477-021-00925-x.doi:10.1038/s41477-021-00925-x
11. Fan X, Qiu H, Han W, et al.Phytoplankton pangenome e senola phetiso e pharalletseng ea liphatsa tsa lefutso tsa prokaryotic tsa mesebetsi e fapaneng.Sci Adv.2020;6(18):eaba0111.E phatlalalitsoe 2020 Apr 29. doi:10.1126/sciadv.aba0111
Nako ea poso: Jan-04-2022