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Litaba

Nanopore-based Full-length Transcriptome Sequencing

Nanopore sequencing e ikhetholla ho li-platform tse ling tsa ho latellana, ka hore li-nucleotide li baloa ka ho toba ntle le motsoako oa DNA 'me li hlahisa nako e telele ho li-kilobase tse mashome.Sena se matlafatsa ho bala ka ho otloloha ha lingoliloeng tsa bolelele bo felletseng le ho sebetsana le mathata a lithuto tsa transcriptomic.

√ Leeme le ikhethileng la tatelano e itseng

√ Palo e felletseng ea cDNA ho ea ho lithuto tsa sebopeho sa liphatsa tsa lefutso

√ Lintlha tse fokolang tse hlokahalang ho koahela palo e tšoanang ea lingoloa

√ Ho tsebahatsa li-isoform tse ngata ka jini

√ Tlhaloso ea polelo ho boemo ba isoform

nanopore-1

Pipeline e tloaelehileng ea Bioinformatics

Bolelele bo felletseng-trans-bix-1024x254

Linyeoe tse Atlehileng Haufinyane ka Biomarker Technologies

1. Litlhahlobo tse bapisoang tsa li-transcriptomes tsa bolelele bo felletseng li senola Gnetum luofuense stem developmental dynamics.

Journal: Frontiers in Genetics

E phatlalalitsoe: Mar. 2021

Mantsoe a bohlokoa: MinION |Alternative splicing |APA |lncRNA |Litlhaku tse hlahisitsoeng ka mokhoa o fapaneng

Bala Mongolo o felletseng

2. Motsamao o le mong oa li-RNA tse nyane ho tloha ho letlobo ho isa metsong ho li-interspecific heterografts

Journal: Limela tsa Tlhaho

E phatlalalitsoe: Pherekhong 2021

Mantsoe a bohlokoa: Nanopore |Ilumina |phumano ea mRNA ea bolelele bo felletseng

3. Tlhahlobo e feletseng ea transcriptome ea metso ea asparagus e senola mokhoa oa molek'hule oa mamello ea letsoai o bakoang ke arbuscular mycorrhizal fungus.

Journal: Botany ea Tikoloho le Teko

E phatlalalitsoe: Pherekhong 2021

Mantsoe a bohlokoa: MinION |Lingoloa tse hlahisitsoeng ka mokhoa o fapaneng |Lintlha tsa mongolo |Alternative splicing

4. Liphetoho tsa Sebopeho sa 'Mele le Phetolelo ea Bolelele bo Feletseng ba Rose (Rosa chinensis) Metso le Makhasi ho Araba Khatello ea Komello.

Journal: Semela le Cell Physiology

E phatlalalitsoe: Oct. 2020

Mantsoe a bohlokoa: PromethION |Lingoloa tse hlahisitsoeng ka mokhoa o fapaneng |Boprofeta bo sebetsang |Li-isoform tse arohaneng |TF le lncRNA

5. Tlhahlobo le papiso e felletseng ea PacBio le tatellano ea RNA e thehiloeng ho nanopore ea Arabidopsis transcriptome

Journal: Mokhoa oa Semela

E phatlalalitsoe: Mmesa 2020

Mantsoe a bohlokoa: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |Khokahano ea liphatsa tsa lefutso |Boitsebiso ba sengoloa |Alternative splicing |SSR |LNCRNA |Isoform quantification

Litaba le Lintlha-khōlō e ikemiselitse ho arolelana linyeoe tsa morao-rao tse atlehileng le Biomarker Technologies, ho hapa likatleho tse ncha tsa mahlale hammoho le mekhoa e tsebahalang e sebelisitsoeng nakong ea thuto.


Nako ea poso: Jan-08-2022

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