MOEKETSI
Tšebelisano ea baktheria le ho se sebetse ka biochar e ntlafalitse ho senyeha ha tebuconazole, sebopeho sa microbiome ea mobu le ho sebetsa.
Bolelele bo felletseng ba 16S amplicon sequencing |PacBio HiFi |Alpha mefuta-futa |Phapang ea Beta
Thutong ena, tatellano ea amplicon ea 16S e bolelele bo felletseng ka PacBio le tlhahlobo ea bioinformatic e fanoe ke Biomarker Technologies.
Lintlha-khōlō
Biochar immobilized tebuconazole-degrading bacteria Alcaligenes faecalis WZ-2 e ile ea ithutoa ka katleho ea biodegradation mme e ama mobu o silafetseng oa tebuconazole ha o bapisoa le mofuta oa mahala o senyang WZ-2.
1. Biochar-immobilized WZ-2 e bonts'a ho senyeha ho hoholo ha tebuconazole ha e bapisoa le mahala ea WZ-2 ka ho fokotsa halofo ea tebuconazole mobung ho tloha matsatsing a 18.7 ho ea ho matsatsi a 13.3.
2. Biochar-immobilized WZ-2 e ile ea khona ho tsosolosa mesebetsi ea enzyme ea mobu oa tlhaho, ho akarelletsa le urease, dehydrogenase le invertase, joalo-joalo.
3. Boemo ba likokoana-hloko mobung o phekotsoeng oa biochar-immobilized WZ-2 o khethiloeng ke ho latellana ha 16S ka ho feletseng ho tšehetsoa ka matla hore tsamaiso ena e ka tsosolosa bophelo ba mobu ka ho ntlafatsa sebopeho sa sechaba sa baktheria tlas'a tšilafalo ea tebuconazole.
Teko (e amanang le tatelano)
Lihlopha: CK: Mobu oa tlhaho;T: Mobu o tšetsoeng ka tebuconazole;S: Tebuconazole e nang le mobu o nang le mathata a mahala WZ-2;BC: Tebuconazole e nang le mobu o nang le biochar;BCS: Tebuconazole e nang le mobu o nang le biochar immobilized WZ-2.
Sampling: Kakaretso ea ho ntšoa ha DNA ea mobu e holisitsoeng ke 16S rDNA primers
27F (5′-AGAGTTTGATCCTGGCTCAG-3′) le 1492R (5′-GGTTACCTTGTTACGA),re percentng bolelele bo felletseng ba 16S rDNA
Sethala sa tatellano: PacBio RS II
Leano la tatellano: CCS HIFI ea bala
Tlhahlobo ea lintlha:BMKCloudBioinformatic Platforming e etsa hore tlhapi ea khauta e be mokhoa o babatsehang oa liphatsa tsa lefutso bakeng sa fisiology ea litlhapi le ho iphetola ha lintho.
Sephetho
Sebopeho sa sechaba sa likokoana-hloko sa mobu se ile sa khethoa ke 16S rDNA tatellano.Lenane la OTU la leruo le mefuta e fapaneng ea alpha, ho kenyeletsoa Chao1, Ace, Shannon le Simpson index li ile tsa hlahlojoa ho senola mefuta-futa ea mefuta tsamaisong ka 'ngoe.Kamora matsatsi a 60 a ho incubation, li-indices tsohle tse bonts'itsoeng mokhoa o ts'oanang, ke hore, tebuconazole e ka fokotsa bongata ba mefuta le mefuta-futa ea mobu.Leha ho le joalo, ka ho eketsa khatello ea WZ-2, sechaba sa libaktheria tsa mobu se ile sa hlaphoheloa ka mokhoa o itseng ho latela maruo le mefuta-futa.Phapang e fokolang e ile ea bonoa har'a BC, BCS le CK, e bontšang hore biochar le biochar-immobilized WZ-2 li ka tsosolosa bophelo bo botle ba mobu ka katleho.
Mokhoa oa sehlopha sa batho ba se nang boima ba 'mele o nang le Arithmetic Means (UPGMA) o sebelisitsoe thutong ena ho bontša phapang ea beta har'a lihlopha.Joalokaha ho bontšitsoe setšoantšong se latelang, BC, BSC le CK li arolelane mokhoa o tšoanang oa sebopeho sa likokoana-hloko ha li bapisoa le sehlopha sa T le S, se ileng sa boela sa bontša hore ho kenngoa ha biochar mobung o silafetseng oa tebuconazole ho ka thusa haholo ho hlaphoheloa ha sechaba sa likokoana-hloko mobung.
Setšoantšo.UPGMA clustering ea sechaba sa libaktheria boemong ba phylum tlas'a phekolo e fapaneng
Referense
Sun, Tong, et al."Ho tsamaisana le baktheria le ho se sebetse ka biochar ho ntlafalitse ho senyeha ha tebuconazole, sebopeho sa microbiome ea mobu le ho sebetsa."Journal of Hazardous Materials398 (2020): 122941.
Litaba le Lintlha-khōlō e ikemiselitse ho arolelana linyeoe tsa morao-rao tse atlehileng le Biomarker Technologies, ho hapa likatleho tse ncha tsa mahlale hammoho le mekhoa e tsebahalang e sebelisitsoeng nakong ea thuto.
Nako ea poso: Jan-08-2022