PHEPO PHETOHO
tlhaho
LITŠOANTŠISO
Tatelano ea liphatsa tsa lefutso e senola litsela tsa ho hasana lefatšeng ka bophara 'me e fana ka maikutlo a phetoho ea liphatsa tsa lefutso ho seahorse evolution.
PacBio |Ilumina |Hi-C |WGS |Liphatsa tsa lefutso |Nalane ea palo ea batho |Phallo ea Gene
Tatelano ea Pacbio, kopano ea genome de novo le lits'ebeletso tsa litlhaloso li fanoe ke Biomarker Technologies.
Lintlha-khōlō
1.Ho ile ha fumanoa genome ea boleng bo phahameng ba chromosome-level seahorse (Hippocampus erectus) e nang le contig N50 ea 15.5 Mb.
2. Kakaretso ea liphatsa tsa lefutso tse 358 ho tsoa lefats'eng ka bophara mefuta e 21 ea li-seahorse e ile ea lateloa.
3. Li-seahorses tse bileng teng ho elella qetellong ea Oligocene le litsela tse ileng tsa latela tsa bokolone ba lefats'e ka bophara lia tsebahala 'me li amahanngoa le ho fetoha ha matla a maqhubu a leoatle le menyetla ea maoatle a paleotemporal.
4.Motheo oa liphatsa tsa lefutso tsa "bony spines" tse feto-fetohang tsa phenotype li amahanngoa le li-substitutes tse ikemetseng ho liphatsa tsa lefutso tsa bohlokoa.
5. Rafting ka maqhubu a leoatle e lefella ho hasana ho fokolang le ho ikamahanya le maemo ka potlako ho thusa ho etsa likolone libakeng tse ncha.
Katleho
Setšoantšo sa 1 Phapang ea liphatsa tsa lefutso le likamano tsa phylogenetic tsa 358 seahorse specimens
aLibaka tsa lisampole tsa li-seahorses tse entsoeng lisampole tse nang le mefuta ea nucleotide (π) ea mefuta e 21 ea li-seahorse ho li-chromosome tse 22.b Sefate se kopaneng se hahiloeng ka li-SNP tsa genome-wide tsa 358 seahorses.Matšoao a phini ea sebaka ho (a) le bokamorao ba lekala ho (b) a lumellana.
Setšoantšo sa 2 Bokolone le nalane ea batho ba li-seahorses
aLikhakanyo tsa nako ea li-phylogenetic tsa mefuta ea li-seahorse tse 21.Botenya ba mela ea lekala bo lumellana le likhakanyo tsa boholo ba baahi (Ne) 'me mebala e bontša mela e fapaneng.Matšoao I–III a bontša lintlha tsa ho lekanya.b–d Litsela tse boletsoeng esale pele tsa bokolone (metsu e mebala) ea lipere tsa leoatle tse ipapisitseng le nako ea ho fapana, kabo, liketsahalo tsa maemo, le maqhubu a leoatle (metsu e tšoeu).b The Indo-Australian Archipelago e ne e le setsi sa tšimoloho (letšoao le lefubelu) la mofuta oa Hippocampus pele li-seahorses li arohanngoa le ho hasana lefatšeng ka bophara 18–23 Ma.Li-Seahorses qalong li ile tsa etsa kolone ea Leoatle la Atlantic ka tsela e bulehileng ea leoatle ea Tethyan, eo, kamora ho koaloa (Ketsahalo ea terminal ka 7-13 Ma), e ileng ea arola leloko lena la Tethyan le leloko la khaitseli ea lona la Leoatle la Indian.Ea morao-rao, e ileng ea fetoha ka potlako (ho tšoaea mosehla) Leoatleng la Arabia, ho theha setsi sa bobeli sa mefuta-futa ea li-seahorse.d Ketsahalo ea bobeli ea kolone ea Leoatle la Atlantic e etsahetse ho tloha Leoatleng la Indian hoo e ka bang 5Ma ka ho feta ntlha ea Afrika Boroa, 'me qetellong ea fihla Leoatleng la Pacific Bochabela ka tsela e ntseng e bulehile ea leoatle ea Panama hoo e ka bang 3.6 Ma.
Setšoantšo sa 3 Phallo ea liphatsa tsa lefutso le ho feto-fetoha ha palo ea baahi e sebetsang hantle
aPhallo ea liphatsa tsa lefutso e fumanoe lipakeng tsa mefuta e lulang Leoatleng la Atlantic Boroa.Phallo ea liphatsa tsa lefutso e bonts'oa haufi le mela e mesoeu joalo ka sekhahla sa ho falla se hlahisitsoeng ke G-PhoCS.Botenya le tataiso ea metsu li lumellana le litekanyetso le tataiso ea phallo ea lefutso, ka ho latellana.b Ho feto-fetoha hoa palo ea baahi e atlehang ke PSMC.Axis ea x e emela nako lilemong tse fetileng ha karolo ea y e emela boholo bo sebetsang ba baahi.Lichate li hlophisitsoe haholo-holo ho latela kabo ea sebaka bakeng sa mofuta o mong le o mong o nang le libaka tse fapaneng tsa kabo.c Phetoho ea boemo ba leoatle nakong ea lilemo tse 1 million tse fetileng ka limithara33.Mola o mosehla o bonts'a tlhoro ea ho qetela ea lefatše ka bophara ha 'mala o mosoeu o bontša nako ea ho qetela ea leqhoa.
Setšoantšo sa 4 Ho iphetola ha mokokotlo.
aKa ho le letšehali, Sefate sa Species se bontšang phetoho e ikemetseng ea li-spines ho li-seahorses.Bolelele ba lekala bo bonts'a palo ea libaka tsa sebaka ka seng.Mefuta e mene ea li-spiny seahorse e totobalitsoe ka boputsoa.Makala a teteaneng a lumellana le litekanyetso tse phahameng tsa li-substitutes tse sa tsejoeng ho ea ho li-synonymous (dN/dS) bakeng sa bmp3 gene.Teko ea Canonical le e akaretsang ea McDonald le Kreitman (MKT) bakeng sa mofuta oa bmp3 e entsoe bakeng sa mefuta e meraro ea likhaitseli tse nang le likarolo tse fapaneng tsa spiny le tse se nang spiny tse totobalitsoeng ke mebala e ka morao, eo maemo a eona a bohlokoa a bonts'itsoeng ka boleng ba p le fonte e putsoa le e khubelu, ka ho latellana.Ka ho le letona, papiso ea li-substitutes tsa amino acid ka protheine ea bmp3, polymorphic le li-substitutes tse tsitsitseng ho li-spiny seahorses li bontšoa ka li-circles tse khubelu le tse putsoa, ka ho latellana.b Kabo ea boleng ba dN/dS ho bmp3 ho li-spiny seahorses ha li bapisoa le mefuta e seng spiny.c Ho iphetola ha lintho ka boikemelo sefateng sa phylogenetic se hahiloeng bocha bakeng sa protheine e kentsoeng ke bmp3.d Ho kopanngoa ha Whole-mount in situ ea bmp3 ho Hippocampus erectus.
Referense
Li C et al.Tatelano ea liphatsa tsa lefutso e senola litsela tsa ho hasana lefatšeng ka bophara 'me e fana ka maikutlo a ho feto-fetoha ha liphatsa tsa lefutso ho seahorse evolution.Nat Commun.2021 Hlakola 17;12(1):1094.
Litaba le Lintlha-khōlō e ikemiselitse ho arolelana linyeoe tsa morao-rao tse atlehileng le Biomarker Technologies, ho hapa likatleho tse ncha tsa mahlale hammoho le mekhoa e tsebahalang e sebelisitsoeng nakong ea thuto.
Nako ea poso: Jan-06-2022