T2T GENOME ASSEMBLY, GAP FREE GENOME
1stLi-Genomes tse peli tsa Rice1
Sehlooho: Kopano le netefatso ea li-Genomes tse peli tse se nang Gap bakeng sa Xian/indica Rice e Senola Litlhaloso ka Mehaho ea Plant Centromere
Doi:https://doi.org/10.1101/2020.12.24.424073
Nako e behiloeng: Pherekhong 01, 2021.
Setsi: Univesithi ea Temo ea Huazhong, Chaena
Lisebelisoa
O. sativa xian/indicamefuta ea raese 'Zhenshan 97 (ZS97)' le 'Minghui 63 (MH63)
Leano la tatellano
NGS e bala + HiFi ea bala + CLR e bala + BioNano + Hi-C
Lintlha:
ZS97: 8.34 Gb (~23x) HiFi e bala + 48.39 Gb (~131x ) CLR e bala + 25 Gb(~69x) NGS + 2 Lisele tsa BioNano Irys
MH63: 37.88 Gb (~103x) HiFi e bala + 48.97 Gb (~132x) CLR e bala + 28 Gb(~76x) NGS + 2 Lisele tsa BioNano Irys
Setšoantšo sa 1 Li-genome tse peli tse se nang lekhalo tsa raese (MH63 le ZS97)
2ndBanana Genome2
Sehlooho: Telomere-to-telomere gapless chromosome ea banana e sebelisa nanopore sequencing
Doi:https://doi.org/10.1101/2021.04.16.440017
Nako e behiloeng: La 17 Mmesa 2021.
Setsi: Université Paris-Saclay, Fora
Lisebelisoa
Haploid e habeliMusa acuminatasppmalacensis(DH-Pahang)
Leano le data:
HiSeq2500 PE250 mode + MinION/ PromethION (93Gb, ~200X )+ Optical map (DLE-1+BspQ1)
Letlapa la 1 Papiso ea likopano tsa genome tsa Musa acuminata (DH-Pahang).
Setšoantšo sa 2 Musa genomes papiso ea meralo
3rdPhaeodactylum tricornutum genome3
Sehlooho: Kopano ea genome ea Telomere-to-telomere eaP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Nako e behiloeng: La 4 Mots'eanong 2021
Setsi: Univesithi ea Bophirimela, Canada
Lisebelisoa
Phaeodactylum tricornutum(Setso Pokello ea Algae le Protozoa CCAP 1055/1)
Leano le data:
1 Oxford Nanopore minion flow cell + e 2 × 75 e phehiloeng bohareng ba sephetho NextSeq 550 run
Setšoantšo sa 3 Phallo ea mosebetsi bakeng sa kopano ea genome ea telomere-to-telomere
4thCHM13 genome ea motho4
Sehlooho: Tatelano e feletseng ea lefutso la motho
Doi:https://doi.org/10.1101/2021.05.26.445798
Nako e behiloeng: La 27 Mots'eanong 2021
Setsi: Mekhatlo ea Sechaba ea Bophelo (NIH), USA
Lisebelisoa: mohala oa sele CHM13
Leano le data:
30 × PacBio circular consensus sequencing (HiFi) , 120 × Oxford Nanopore ultra-long bala sequencing , 100 × Illumina PCR-Free sequencing (ILMN) , 70 × Illumina / Arima Genomics Hi-C (Hi-C), BioNano le Strand-seq
Letlapa la 2 Papiso ea likopano tsa genome tsa batho tsa GRCh38 le T2T-CHM13
Referense
1.Sergey Nurk et al.Tatelano e felletseng ea lefutso la motho.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser et al.Telomere-to-telomere gapless chromosome ea banana e sebelisang nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere et al.Kopano ea Genome ea Telomere-to-telomere ea Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Song et al.Ho Kopana le ho Tiisoa ha Li-Genomes tse peli tsa Reference tse se nang Gap bakeng sa Xian/indica Rice e Senola Litlhaloso ka Mehaho ea Plant Centromere.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Nako ea poso: Jan-06-2022