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Litaba

T2T GENOME ASSEMBLY, GAP FREE GENOME

1stLi-Genomes tse peli tsa Rice1

Sehlooho: Kopano le netefatso ea li-Genomes tse peli tse se nang Gap bakeng sa Xian/indica Rice e Senola Litlhaloso ka Mehaho ea Plant Centromere

Doi:https://doi.org/10.1101/2020.12.24.424073

Nako e behiloeng: Pherekhong 01, 2021.

Setsi: Univesithi ea Temo ea Huazhong, Chaena

Lisebelisoa

O. sativa xian/indicamefuta ea raese 'Zhenshan 97 (ZS97)' le 'Minghui 63 (MH63)

Leano la tatellano

NGS e bala + HiFi ea bala + CLR e bala + BioNano + Hi-C

Lintlha:

ZS97: 8.34 Gb (~23x) HiFi e bala + 48.39 Gb (~131x ) CLR e bala + 25 Gb(~69x) NGS + 2 Lisele tsa BioNano Irys

MH63: 37.88 Gb (~103x) HiFi e bala + 48.97 Gb (~132x) CLR e bala + 28 Gb(~76x) NGS + 2 Lisele tsa BioNano Irys

Setšoantšo-1

Setšoantšo sa 1 Li-genome tse peli tse se nang lekhalo tsa raese (MH63 le ZS97)

2ndBanana Genome2

Sehlooho: Telomere-to-telomere gapless chromosome ea banana e sebelisa nanopore sequencing

Doi:https://doi.org/10.1101/2021.04.16.440017

Nako e behiloeng: La 17 Mmesa 2021.

Setsi: Université Paris-Saclay, Fora

Lisebelisoa

Haploid e habeliMusa acuminatasppmalacensis(DH-Pahang)

Leano le data:

HiSeq2500 PE250 mode + MinION/ PromethION (93Gb, ~200X )+ Optical map (DLE-1+BspQ1)

Letlapa la 1 Papiso ea likopano tsa genome tsa Musa acuminata (DH-Pahang).

Lethathamo la 1-Papiso-ea-GRCh38-le-T2T-CHM13-likopano tsa genome tsa motho
Setšoantšo-Musa-genomes-architecture-papiso

Setšoantšo sa 2 Musa genomes papiso ea meralo

3rdPhaeodactylum tricornutum genome3

Sehlooho: Kopano ea genome ea Telomere-to-telomere eaP
haeodactylum tricornutum

Doi:https://doi.org/10.1101/2021.05.04.442596

Nako e behiloeng: La 4 Mots'eanong 2021

Setsi: Univesithi ea Bophirimela, Canada

Lisebelisoa

Phaeodactylum tricornutum(Setso Pokello ea Algae le Protozoa CCAP 1055/1)

Leano le data:

1 Oxford Nanopore minion flow cell + e 2 × 75 e phehiloeng bohareng ba sephetho NextSeq 550 run

Figure-Workflow-for-telomere-to-telomere-genome-assembly-1-1024x740

Setšoantšo sa 3 Phallo ea mosebetsi bakeng sa kopano ea genome ea telomere-to-telomere

4thCHM13 genome ea motho4

Sehlooho: Tatelano e feletseng ea lefutso la motho

Doi:https://doi.org/10.1101/2021.05.26.445798

Nako e behiloeng: La 27 Mots'eanong 2021

Setsi: Mekhatlo ea Sechaba ea Bophelo (NIH), USA

Lisebelisoa: mohala oa sele CHM13

Leano le data:

30 × PacBio circular consensus sequencing (HiFi) , 120 × Oxford Nanopore ultra-long bala sequencing , 100 × Illumina PCR-Free sequencing (ILMN) , 70 × Illumina / Arima Genomics Hi-C (Hi-C), BioNano le Strand-seq

Letlapa la 2 Papiso ea likopano tsa genome tsa batho tsa GRCh38 le T2T-CHM13

Tafole-Papiso-of-Musa-acuminata-DH-Pahang-genome-assemblies

Referense

1.Sergey Nurk et al.Tatelano e felletseng ea lefutso la motho.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798

2.Caroline Belser et al.Telomere-to-telomere gapless chromosome ea banana e sebelisang nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017

3.Daniel J. Giguere et al.Kopano ea Genome ea Telomere-to-telomere ea Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596

4.Jia-Ming Song et al.Ho Kopana le ho Tiisoa ha Li-Genomes tse peli tsa Reference tse se nang Gap bakeng sa Xian/indica Rice e Senola Litlhaloso ka Mehaho ea Plant Centromere.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073


Nako ea poso: Jan-06-2022

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