Litaba

Matlafatso ea Transposable element (TE) e amohetsoe e le matla a tsamaisang katoloso ea boholo ba genome le ho iphetola ha lintho, empa litlamorao tsa ho bopa boqapi ba 3D genomic li ntse li sa tsejoe haholo limela.Mona, re tlaleha likopano tsa mofuta oa liphatsa tsa lefutso bakeng sa mefuta e meraro ea k'hothone ho tloha ka makhetlo a mararo ka boholo ba genome, e leng.Gossypium rotundifolium(K2),G. arboreum(A2), leG. raimondii(D5), ho sebelisa Oxford Nanopore Technologies.Papiso ea genome e hlahlobisisa lintlha tsa ho holisa TE ka mokhoa o ikhethileng oa leloko ho tlatsetsang phapanong e kholo ea boholo ba genome (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), 'me e bonts'a likahare tsa liphatsa tsa lefutso tse baballang le likamano tsa synteny har'a liphatsa tsa lefutso.Re fumane hore hoo e ka bang 17% ea liphatsa tsa lefutso tsa syntenic li bonts'a phetoho ea boemo ba chromatin lipakeng tsa likarolo tse sebetsang ("A") le tse sa sebetseng ("B"), 'me matlafatso ea TE e ne e amahanngoa le keketseho ea karolo ea A karolong ea liphatsa tsa lefutso (~ 7,000 liphatsa tsa lefutso. ) ho K2 le A2 mabapi le D5.Ke 42% feela ea meeli ea topologically associating domain (TAD) e bolokiloeng har'a liphatsa tsa lefutso tse tharo.Lintlha tsa rona li ama katoloso ea morao-rao ea li-TE ka mor'a ho theoa ha meeli ea TAD e ikhethileng.Boithuto bona bo fana ka leseli mabapi le karolo ea katoloso ea genome ea transposon-mediated phetohong ea sebopeho sa chromatin se maemong a holimo limeleng.