High-throughput genotyping, haholo-holo ho batho ba bangata, ke mohato oa bohlokoa lithutong tsa liphatsa tsa lefutso, tse fanang ka motheo oa liphatsa tsa lefutso bakeng sa ho sibolla liphatsa tsa lefutso tse sebetsang, tlhahlobo ea ho iphetola ha lintho, joalo-joalo Ho e-na le ho latellana ho tebileng ha liphatsa tsa lefutso, ho fokotsoa ha tatellano ea liphatsa tsa lefutso (RRGS). ) e hlahisoa ho fokotsa litšenyehelo tsa tatellano ea sampuli ka 'ngoe, ha ho ntse ho boloka ts'ebetso e utloahalang ho sibolloeng ha lets'oao la lefutso.Hangata sena se finyelloa ka ho ntša sekhechana sa lithibelo ka har'a boholo bo fanoeng, se bitsoang laeborari ea boemeli bo fokolitsoeng (RRL).Specific-locus amplified fragment sequencing(SLAF-Seq) ke leano le iketselitseng la ho sibolla de novo SNP le SNP genotyping ea batho ba bangata.
Ts'ebetso ea tekheniki
SLAF vs mekhoa e teng ea RRL
Melemo ea SLAF
Bokhoni bo phahameng ba ho sibolla liphatsa tsa lefutso- Ha e kopantsoe le theknoloji ea tatellano ea maemo a holimo, SLAF-Seq e ka fihlela li-tag tse likete tse makholo tse fumanoeng ka har'a genome eohle ho phethahatsa kopo ea merero e fapaneng ea lipatlisiso, ebang ke ka genome ea rona kapa ka eona.
Moralo oa liteko o ikhethileng le o feto-fetohang- Bakeng sa sepheo kapa mefuta e fapaneng ea lipatlisiso, maqheka a fapaneng a tšilo ea enzymatic a fumaneha ho kenyelletsa le enzyme e le 'ngoe, li-enzyme tse peli le tšilo ea li-enzyme tse ngata.Leano la tšilo ea lijo le tla hlahlojoa esale pele ka silika ho netefatsa moralo o nepahetseng oa enzyme.
Ts'ebetso e phahameng ea tšilo ea enzymatic- Tšilo e entsoeng esale pele ea enzymatic e fana ka li-SLAF tse ajoang ka ho lekana ho chromosome.Pokello ea likhechana e sebetsang hantle e ka fihlella ho feta 95%.
Qoba ho pheta-pheta tatellano- Peresente ea tatellano e pheta-phetoang ho data ea SLAF-Seq e fokotsehile ho ea tlase ho 5%, haholo-holo mefuta e nang le boemo bo phahameng ba lintho tse iphetang, tse kang koro, poone, joalo-joalo.
Boikemisetso ba ts'ebetso ea bioinformatics e tsoetseng pele- BMK e ntlafalitse ts'ebetso e kopaneng ea bioinformatic e sebetsang ho theknoloji ea SLAF-Seq ho netefatsa ts'epo le ho nepahala ha tlhahiso ea ho qetela.
Tšebeliso ea SLAF
'Mapa oa khokahano oa liphatsa tsa lefutso
Kaho ea 'mapa oa liphatsa tsa lefutso o matla haholo le ho tsebahatsa sebaka se laolang mekhoa ea mofuta oa lipalesa ho Chrysanthemum(Chrysanthemum x morifolium Ramat.)
Journal: Horticulture Research e Phatlalalitsoeng: 2020.7
GWASE
Ho tsebahatsa mofuta oa mokhethoa o amanang le litaba tsa isofavone peong ea soybean ho sebelisoa motsoako oa genome-wide le 'mapa oa khokahano.
Journal: Plant Journal E Phatlalalitsoeng: 2020.08
Liphatsa tsa lefutso tsa ho iphetola ha lintho
Population genomic analysis and de novo assembly e senola tšimoloho ea raese ea mofoka e le papali ea ho iphetola ha lintho
Journal: Semela sa Limolek'hule se Phatlalalitsoeng: 2019.5
Bulked Segregant Analysis (BSA)
GmST1, e kenyelletsang sulfotransferase, e fana ka khanyetso ho mefuta ea kokoana-hloko ea soybean mosaic G2 le G3.
Journal: Plant, Cell & Tikoloho E Phatlalalitsoeng: 2021.04
Referense
Sun X, Liu D, Zhang X, le ba bang.SLAF-Seq: mokhoa o sebetsang hantle oa ho sibolloa ha SNP ka tekanyo e kholo ea de novo le genotyping o sebelisa tatellano ea maemo a holimo[J].Plos one, 2013, 8(3):e58700
Song X, Xu Y, Gao K, et al.Kaho ea 'mapa oa liphatsa tsa lefutso o phahameng haholo le ho tsebahatsa loci e laolang litšobotsi tsa mofuta oa lipalesa ho Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.2020;7:108.
Wu D, Li D, Zhao X, et al.Ho tsebahatsa mofuta oa mokhethoa o amanang le litaba tsa isoflavone dipeong tsa soya ho sebelisoa kamano e pharalletseng ea genome le 'mapa oa khokahano.Semela J. 2020;104(4): 950-963.
Sun J, Ma D, Tang L, et al.Population Genomic Analysis le Kopano ea De Novo e Senola Tšimoloho ea Weedy Rice e le Papali ea Tsoelo-pele.Semela sa Mol.2019;12(5):632-647.Semela sa Mol.2018;11(11):1360-1376.
Zhao X, Jing Y, Luo Z, et al.GmST1, e kenyelletsang sulfotransferase, e fana ka khanyetso ho mefuta ea kokoana-hloko ea soybean mosaic G2 le G3.Tikoloho ea Cell ea Semela.2021;10.1111/pce.14066
Nako ea poso: Jan-04-2022