Ho hlahloba mekhoa ea liphatsa tsa lefutso sebakeng sa pele sa ntho e phelang ho bohlokoa bakeng sa ho utloisisa mefuta le mesebetsi ea lisele tsa eona.Leha ho le joalo, mekhoa ea hajoale ea tlhahlobo ea transcriptome ea sebaka e na le mefokolo joalo ka ho fokotseha kapa tharollo e sa lekaneng.BMKMANU S1000 Spatial Chip, e ntlafalitsoeng ke BMKMANU, e khona ho bona tlhaiso-leseling ea liphatsa tsa lefutso tsa in-situ likarolong tse felletseng tsa li-subcellular resolution, e nolofalletsang tlhaloso e fapaneng ea sebopeho sa lisele.
Itokisetse ho bona sehlahisoa sa rona se secha!Ho keteka ho lokolloa ha eona, re fana ka e khethehilengReka Tse Nne Fumana E 'Ngoe Mahalapapatso ea nako e lekanyelitsoeng feela.
Likaroloana tsa Sehlahisoa
BMKMANU S1000 chip ea sebaka le lisebelisoa tse tšoanang tsa reagent li kenyelletsa:
1) Tissue Optimization Kit, e nang le li-reagents bakeng sa ntlafatso ea lisele (tlhahlobo e nepahetseng ea nako bakeng sa phepelo ea lisele).
2) Gene Expression Kit, e nang le li-reagents bakeng sa ho hapa mRNA ka lilae tsa linama bakeng sa tokiso le tatellano ea laeborari e latelang.
3) Kit ea ho Qala: E kenyelletsa adapter ea thermostatic le transcriptome magnetic separator.
Theknoloji Molao-motheo
Chip e ipapisitse le li-micropores le li-microbeads, 'me mRNA e ka nkuoa e le situ ka Oligo ho li-microbeads.Oligo e entsoe ka likarolo tse 'nè: Read1, Spatial Barcode, UMI, le Poly(dT)VN.Ka mor'a hore lesela le khomaretsoe ho chip, mRNA e lokolloa liseleng ka enzyme ea permeabilization.Kaha boholo ba lipheletso tsa mRNA 3' li na le mohatla oa Poly-A, li hapiloe ke Oligo ka Poly(dT)VN.Kamora ho holisoa ha RT-PCR le cDNA, ho lokisoa ha laeborari le tatellano, ho lekola boemo ba sebaka ho fihlelleha ka Spatial Barcode.Sena se lumella tlhahlobo ea polelo ea liphatsa tsa lefutso libakeng tsa sebaka le ho fapana ha lisele.
Melemo ea Sehlahisoa
Lisebelisoa
E ka sebelisoa libakeng tse ngata tsa lipatlisiso tsa baeloji le tsa bongaka, ho kenyeletsoa hlahala, lefu, immunology, le biology ea nts'etsopele, e thusa ho fihlela katleho e ncha mafapheng ana ka tlhahlobo ea polelo ea sebaka ho qeto ea subcellular.
● Hlahala le Boloetse:
Sepakapaka sa heterogeneity le microenvironment ea lihlahala le mafu
Ho qala le nts'etsopele ea lihlahala le mafu
Karabelo ea kalafo ea lihlahala le mafu
●Baeloji ea Tsoelo-pele
Spatiotemporal atlas ea litho tsa 'mele
Mekhoa ea ho laola liphatsa tsa lefutso nakong ea tsoelo-pele
● Karabelo ea Khatello ea Maikutlo
Karabelo ea khatello ea kelello ea biotic
Karabelo ea khatello ea maikutlo
● Thuto ea ho itšireletsa mafung
Karabelo ea 'mele oa ho itšireletsa mafung ka ho kenya setho sa 'mele
Mekhoa ea ho itšireletsa mafung ea lihlahala le mafu
Pathogenesis ea mafu a autoimmune
● Tlhahlobo ea ho hanyetsa lithethefatsi
Mekhoa ea ho hanyetsa lithethefatsi
Lipatlisiso le nts'etsopele ea meriana e mecha
Linyeoe le Demo Data
BMKMANU S1000 e netefalitsoe ts'ebetso maemong a makholo ho pholletsa le mefuta e fapaneng ea lisele.
