Liphatsa tsa lefutso tsa ho iphetola ha lintho
Melemo ea Tšebeletso
Takagi et al.,Koranta ea semela, 2013
● Ho hakanya nako ea ho fapana ha mefuta le lebelo ho ipapisitsoe le ho fapana ha li-nucleotide le li-amino acid.
● Ho hlahisa kamano e tšepahalang haholoanyane ea phylogenetic pakeng tsa mefuta e nang le tšusumetso e fokolang ea ho iphetola ha lintho le ho bapisa ho iphetola ha lintho.
● Ho theha maqhama pakeng tsa liphetoho tsa liphatsa tsa lefutso le phenotypes ho senola liphatsa tsa lefutso tse amanang le tšobotsi
● Ho lekanya mefuta-futa ea liphatsa tsa lefutso, e bontšang bokhoni ba ho iphetola ha lintho
● Nako ea ho Fetolela ka Potlako
● Boiphihlelo bo pharaletseng: BMK e bokelletse phihlelo e kholo mererong e amanang le palo ea baahi le ea ho iphetola ha lintho ka lilemo tse fetang 12, e akaretsang makholo a mefuta ea liphoofolo, joalo-joalo, 'me e kentse letsoho mererong e fetang 80 ea boemo bo holimo e hatisitsoeng ho Nature Communications, Molecular Plants, Plant Biotechnology Journal, joalo-joalo.
Litlhaloso tsa Tšebeletso
Lisebelisoa:
Ka tlwaelo, ho kgothaletswa bonyane dihlopha tse tharo tse nyane (mohlala, mefuta e nyane kapa mefuta).Sebaka se seng le se seng sa baahi se lokela ho ba le batho ba seng ka tlase ho 10 (Limela ~15, li ka fokotsoa bakeng sa mefuta e sa tloaelehang).
Leano la tatellano:
* WGS e ka sebelisoa bakeng sa mefuta e nang le genome ea boleng bo holimo, ha SLAF-Seq e sebetsa ho mefuta e nang le genome kapa ntle le genome, kapa genome ea boleng bo tlase.
| E sebetsa ho boholo ba genome | WGS | Li-tag tsa SLAF (×10,000) |
| ≤ 500 Mb | 10×/ka motho ka mong | WGS e khothaletsoa ho feta |
| 500 Mb - 1 Gb | 10 | |
| 1 Gb - 2 Gb | 20 | |
| ≥2 Gb | 30 |
Litlhahlobo tsa Bioinformatics
● Tlhahlobo ea ho iphetola ha lintho
● Ho fiela ka boikhethelo
● Phallo ea liphatsa tsa lefutso
● Histori ea palo ea batho
● Nako ea ho arohana
Litlhoko tsa Mohlala le Phano
Litlhoko tsa Mohlala:
| Mefuta | Tishu | WGS-NGS | SLAF |
| Phoofolo
| Lisele tsa visceral |
0.5 ~ 1g
|
0.5g
|
| Lithishu tsa mesifa | |||
| Mali a mammalian | 1.5mL
| 1.5mL
| |
| Mali a likhoho/Litlhapi | |||
| Semela
| Lekhasi le Lecha | 1 ~ 2g | 0.5 ~ 1g |
| Petale/Stem | |||
| Motso/Peo | |||
| Lisele | Seleng ea setso |
| gDNA | Ho tsepamisa maikutlo | Chelete (ug) | OD260/OD280 |
| SLAF | ≥35 | ≥1.6 | 1.6-2,5 |
| WGS-NGS | ≥1 | ≥0.1 | - |
Phallo ea Mosebetsi oa Ts'ebeletso
Moqapi oa liteko
Mohlala oa thomello
Kaho ea laebrari
Tatelano
Tlhahlobo ea lintlha
Litšebeletso tsa ka mor'a thekiso
*Liphetho tsa demo tse bontšitsoeng mona kaofela li tsoa ho liphatsa tsa lefutso tse hatisitsoeng ka BMKGENE
1.Tlhahlobo ea ho iphetola ha lintho e na le kaho ea sefate sa phylogenetic, sebopeho sa baahi le PCA e thehiloeng ho liphatsa tsa lefutso.
Sefate sa Phylogenetic se emela likamano tsa taxonomic le tsa ho iphetola ha lintho har'a mefuta e nang le moholo-holo.
PCA e ikemiselitse ho bona kamano e haufi pakeng tsa batho ba tlase.
Sebopeho sa baahi se bontša boteng ba palo e nyenyane ea liphatsa tsa lefutso ho latela maqhubu a allele.
Chen, le.al.,PNAS, 2020
2.Selective fiela
Khetho e khethollang e bolela mokhoa oo ka oona sebaka se molemo se khethoang le ho eketseha ha libaka tsa libaka tse sa jeleng paate 'me tsa libaka tse sa amaneng li fokotseha, e leng se bakang phokotso ea libaka.
Ho sibolla ha Genome ka bophara libakeng tse ikhethileng ho sebetsoa ka ho bala palo ea liphatsa tsa lefutso tsa palo ea batho (π, Fst, Tajima's D) ea li-SNP tsohle ka har'a fensetere e thellang (100 Kb) mohatong o itseng (10 KB).
Phapang ea Nucleotide (π)
Tajima D
Lenane la ho lokisa(Fst)
Wu, le.al.,Semela sa Molek'hule, 2018
Phallo ea 3.Gene
Wu, le.al.,Semela sa Molek'hule, 2018
Histori ea 4.Demographic
Zhang, le.al.,Nature Ecology & Evolution, 2021
5. Nako ea ho arohana
Zhang, le.al.,Nature Ecology & Evolution, 2021
Taba ea BMK
'Mapa oa phetoho ea liphatsa tsa lefutso o fana ka leseli mabapi le motheo oa liphatsa tsa lefutso tsa khetho ea Spring Chinese Cabbage(Brassica rapa ssp. Pekinensis)
E hatisitsoe: Semela sa Molek'hule, 2018
Leano la tatellano:
Ho latelana: botebo ba tatellano: 10 ×
Liphetho tsa bohlokoa
Phuputsong ena, ho ile ha sebetsoa likhabeche tsa Machaena tse 194 bakeng sa ho hlophisoa bocha ka botebo bo boholo ba 10×, tse hlahisitseng 1,208,499 SNPs le 416,070 InDels.Tlhahlobo ea Phylogenetic meleng ena e 194 e bontšitse hore mela ena e ka aroloa ka mefuta e meraro ea ecotypes, selemo, lehlabula le hoetla.Ho feta moo, sebopeho sa baahi le tlhahlobo ea PCA e bonts'itse hore k'habeche ea selemo ea China e simolohile ho khabeche ea hoetla Shandong, China.Ka mor'a moo, tsena li ile tsa tsebisoa Korea le Japane, tsa tšela mela ea sebaka seo 'me mefuta e meng ea tsona ea morao-rao e ile ea khutlisetsoa Chaena 'me qetellong ea fetoha k'habeche ea Spring Chinese.
Ho hlahlojoa ha Genome ka har'a likhabeche tsa selemo tsa selemo le likhabeche tsa hoetla ha ho khethoa ho senotse libaka tse 23 tsa genomic tse fetileng khethong e matla, tse peli tsa tsona li ne li tsamaisana le sebaka sa taolo ea nako e thehiloeng ho 'mapa oa QTL.Libaka tsena tse peli li fumanoe li na le liphatsa tsa lefutso tsa bohlokoa tse laolang palesa, BrVIN3.1 le BrFLC1.Liphatsa tsena tse peli tsa lefutso li ile tsa netefatsoa hape hore li kentse letsoho ho beheng nako ka boithuto ba transcriptome le liteko tsa transgenic.
 Tlhahlobo ea sebopeho sa baahi ka likhabeche tsa China |  Boitsebiso ba liphatsa tsa lefutso mabapi le khetho ea k'habeche ea Chaena |
Tongbing, le ba bang."'Mapa oa Phapang ea Genomic o Fana ka Lintlha mabapi le Motheo oa Liphatsa tsa lefutso tsa Spring Chinese Cabbage (Brassica rapa ssp.pekinensis)Khetho."Limela tsa molek'hule,11(2018): 1360-1376.
