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Sequencing ea Chromatin Immunoprecipitation (ChIP-seq)
ChIP-Seq e fana ka profil ea genome-wide ea lipheo tsa DNA bakeng sa phetoho ea histone, lintlha tsa transcription, le liprotheine tse ling tse amanang le DNA.E kopanya khetho ea chromatin immuno-precipitation (ChIP) ho khutlisa protheine-DNA complexes, ka matla a tatellano ea moloko o latelang (NGS) bakeng sa tatellano e phahameng ea DNA e fumanoeng.Ho feta moo, kaha li-protein-DNA complexes li fumanoa liseleng tse phelang, libaka tse tlamang li ka bapisoa ka mefuta e sa tšoaneng ea lisele le lisele, kapa tlas'a maemo a sa tšoaneng.Likopo li fapana ho tloha taolong ea mongolo ho ea ho litsela tsa nts'etsopele ho isa mekhoeng ea mafu le ho feta.
Sethala: Illumina NovaSeq 6000
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Tatelano e felletseng ea genome bisulfite
DNA methylation sebakeng sa bohlano ho cytosine (5-mC) e na le tšusumetso e kholo ho polelo ea liphatsa tsa lefutso le tšebetso ea lisele.Mekhoa e sa tloaelehang ea methylation e 'nile ea amahanngoa le maemo le maloetse a mangata, a kang kankere.WGBS e fetohile tekanyetso ea khauta ea ho ithuta genome-wide methylation ka qeto ea motheo e le 'ngoe.
Sethala: Illumina NovaSeq6000
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Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
ATAC-seq ke mokhoa oa tatellano ea maemo a holimo bakeng sa tlhahlobo ea phihlello ea genome-wide chromatin, e leng bohlokoa bakeng sa taolo ea lefats'e ea epigenetic ea polelo ea liphatsa tsa lefutso.Li-adapter tsa tatellano li kenngoa libakeng tse bulehileng tsa chromatin ka hyperactive Tn5 transposase.Kamora ho holisoa ha PCR, ho etsoa laeborari ea tatellano.Libaka tsohle tse bulehileng tsa chromatin li ka fumanoa tlas'a boemo bo itseng ba nako ea sebaka, eseng feela libakeng tse tlamang tsa transcription factor, kapa sebaka se itseng se fetotsoeng sa histone.
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Representation Bisulfite Sequencing (RRBS)
Lipatlisiso tsa DNA methylation esale e le taba e chesang haholo lipatlisisong tsa mafu, 'me e amana haufi-ufi le liphatsa tsa lefutso le litšobotsi tsa pheno-typic.RRBS ke mokhoa o nepahetseng, o sebetsang hantle le oa moruo bakeng sa lipatlisiso tsa DNA methylation.Ntlafatso ea libaka tsa lihlekehleke tsa CpG ka enzymatic cleavage (Msp I), e kopantsoeng le tatellano ea Bisulfite, e fana ka tlhahlobo e phahameng ea DNA methylation.
Sethala: Illumina NovaSeq 6000
