Lihlahisoa

DNA / RNA Sequencing - Nanopore Sequencer

Sequencing ea ONT ke molek'hule e le 'ngoe ea nako ea sebele ea theknoloji ea tatellano ea motlakase e thehiloeng ho nanopores, molao-motheo oa tatellano oa sethala ka seng o tšoana.DNA/RNA e nang le likhoele tse peli e tla tlama ho protheine ea nanoporous e kentsoeng ka har'a biofilm ebe e phutholoha tlas'a boetapele ba protheine ea motlakase, tlasa ts'ebetso ea phapang ea motlakase ho tsoa mahlakoreng ka bobeli a biofilm, likhoele tsa DNA/RNA li feta protheine ea kanal ea nanopore sebakeng se itseng. sekhahla.Ka lebaka la phapang ea lik'hemik'hale tsa metheo e fapaneng holim'a DNA / RNA strand, ha setsi se le seng kapa molek'hule ea DNA e feta mocha oa nanopore, e tla baka phetoho ea lipontšo tse fapaneng tsa motlakase.Ka ho lemoha le ho lumellana le lipontšo tsena, mefuta ea motheo e lumellanang e ka baloa, 'me ho lemoha nako ea sebele ea tatellano ho ka phethoa.

Iteanye le rona

Lintlha tsa Tšebeletso

Sephetho sa Demo

Lintlha tsa Tšebeletso Likarolo

Sethala	Boholo ba Laebrari	Theoretical Data Yield (Per Cell)	Ho nepahala ha Motheo o le Mong	Lisebelisoa
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Seleng	85-92%	SV call, De novo, tatellano ea bolelele bo felletseng, Iso-Seq, tlhaloso ea Gene, Ho fumanoa ha DNA methylation

Melemo ea Tšebeletso

● Boiphihlelo ba lilemo tse fetang 5 sethaleng sa tatellano sa PacBio le likete tsa merero e koetsoeng e nang le mefuta e fapaneng.
● BMKGENE ke molekane oa molao oa Oxford Nanopore, ea nang le setifikeiti sa marang-rang sa RNA / DNA.
● Ho na le mefuta e tloaelehileng ea li-sequencers tse nang le lisebelisoa tse feletseng le mokhoa o lekaneng oa ho latellana.
● Ho ipapisitsoe le sethala sa Nanopore, lipatlisiso tse fetang 10 tsa liphoofolo le limela tsa Denovo li hatisitsoe likoranteng tse tsebahalang lefatšeng ka bophara.

Litlhoko tsa Mohlala

Mofuta oa Mohlala

Chelete

Concentrtion(Qubit ®)

Bolumo

Bohloeki

Ba bang

Genomic DNA

Itšetlehile ka Tlhokahalo ea Lintlha

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

Tlhōrō e hlakileng ho 260 nm, ha ho na litšila

Maikutlo a lokela ho lekanyetsoa ka Qubit le Qubit/Nanopore ≤ 2

Kakaretso ea RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5;ha ho na litšila

RIN boleng ≥7.5

Ts'ebetso ea ts'ebetso ea litšebeletso

Ho lokisetsa mohlala

Kaho ea laebrari

Tatelano

Tlhahlobo ea lintlha

Phano ea morero

E fetileng: DNA / RNA Sequencing - Illumina Sequencer

E 'ngoe: Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Tlhahlobo ea Boleng ba Lintlha tsa Sample ea DNA

Letlapa la 1. Lipalopalo tsa data e hloekileng.

BMKID	rawSeqNum	rawSumBase	hloekisaSeqNum	hloekisaSumBase	cleanN50Len	cleanN90Len	hloekisaMeanLen	hloekisaMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9