Illumina le BGI
Likaroloana
●Platform:Illumina NovaSeq 6000, NovaSeq , HiSeq X Ten le BGI-DNB-T7
●Mekhoa ea tatellano:PE50, PE100, PE150, PE250
●Taolo ea boleng ba lilaebrari pele ho tatellano
●Ho lekanya ho fana ka data le QC:ho fana ka tlaleho ea QC le data e tala ka sebopeho sa fastq ka mor'a ho bala le ho sefa Q30.
Melemo ea Tšebeletso
●Litšebeletso tse fapaneng tsa Sequencing:moreki a ka khetha ho tatellano ka lane, phallo sele kapa palo ea data.
●Mefuta e fapaneng ea li-platform:Lilaebrari tsa DNB li ka fetisetsoa ho li-platform tsa Illumina
●Boiphihlelo bo pharalletseng sethaleng sa tatellano sa Illumina:ka likete tsa merero e koetsoeng ka mefuta e fapaneng.
●Tlhahiso ea tlaleho ea tatellano ea QC:ka metrics ea boleng, ho nepahala ha data le ts'ebetso e akaretsang ea morero oa tatellano.
●Ts'ebetso ea tatellano e holileng:ka nako e khuts'oane ea ho tsamaea.
●Taolo ea Boleng e Tiileng: re kenya ts'ebetsong litlhoko tse tiileng tsa QC ho netefatsa ho fana ka liphetho tsa boleng bo holimo kamehla.
Litlhoko tsa Mohlala*
Karolo e sa Feleng ea Lane Sequencing
| Chelete ea data (X) | Khokahano (qPCR/nM) | Bolumo |
| X ≤ 50 Gb | ≥ 2 nM | ≥ 20 μl |
| 50 Gb ≤ X <100 Gb | ≥ 3 nM | ≥ 20 μl |
| X ≥ 100 Gb | ≥ 4 nM | ≥ 20 μl |
Single Lane (Illumina)
| Sethala | Khokahano (qPCR/nM) | Bolumo |
| HiSeq X Leshome | ≥ 2 nM | ≥ 20 μl |
| NovaSeq 6000 SP | ≥ 1 nM | ≥ 25 μl |
| NovaSeq 6000 S4 | ≥ 1.5 nM | ≥ 25 μl |
| NovaSeq X | ≥ 1.5 nM | ≥ 25 μl |
| BGI-DNBSEQ-T7 | ≥ 1.5 nM | ≥ 25 μl |
Ntle le ho tsepamisa mohopolo le palo eohle, ho boetse ho hlokahala mohlala o loketseng oa tlhoro.
Ka kopo ikopanye le rona haeba disampole tsa hau li sa fihlelle litlhoko tsa ho qala.
Ts'ebetso ea ts'ebetso ea litšebeletso
Taolo ea boleng ba laebrari
Tatelano
Taolo ea boleng ba data
Phano ea morero
Tlaleho ea Library QC
Tlaleho ea boleng ba laebrari e fanoa pele ho tatellano, ho lekola palo ea laebrari, le ho aroloa.
Ho latela tlaleho ea QC
Letlapa la 1. Lipalopalo tsa tatellano ea lintlha.
| ID ea mohlala | BMKID | Li-raw bala | Raw Data (bp) | Ho bala hantle (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Setšoantšo sa 1. Kabo ea boleng hammoho le lipalo ho sampole ka 'ngoe
Setšoantšo sa 2. Kabo ea litaba tsa motheo
Setšoantšo sa 3. Kabo ea litaba tse baloang ka tatellano ea data
