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Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq) Setšoantšo se Featured
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

ATAC-seq ke mokhoa oa tatellano ea maemo a holimo bakeng sa tlhahlobo ea phihlello ea genome-wide chromatin, e leng bohlokoa bakeng sa taolo ea lefats'e ea epigenetic ea polelo ea liphatsa tsa lefutso.Li-adapter tsa tatellano li kenngoa libakeng tse bulehileng tsa chromatin ka hyperactive Tn5 transposase.Kamora ho holisoa ha PCR, ho etsoa laeborari ea tatellano.Libaka tsohle tse bulehileng tsa chromatin li ka fumanoa tlas'a boemo bo itseng ba nako ea sebaka, eseng feela libakeng tse tlamang tsa transcription factor, kapa sebaka se itseng se fetotsoeng sa histone.


Lintlha tsa Tšebeletso

Bioinformatics

Demo Results

Melemo ea Tšebeletso

● Tlhahlobo e Feletseng: Phaephe ea tlhahlobo ea litsebi ho finyella litlhoko tse fapaneng tsa tlhahlobo e hlophisitsoeng;

● Boiphihlelo bo Batsi: Merero e makholo e phethiloe ka katleho ho tla fihlela joale, ho kenyeletsoa lisampole tsa liphoofolo le limela.

Litlhaloso tsa Tšebeletso

Sethala: sethala sa Illumina NovaSeq

Mofuta oa laebrari: ATAC-seq

Tlhahiso ea data e khothalelitsoeng: ≥20M e baloa

Litlhoko tsa Mohlala

Mofuta oa mohlala: Lithane, lisele tse phelang, kapa lisele tse hatselitsoeng joalo-joalo

Nomoro ea sele: ≥ 5×105lisele, nomoro ea sele e nepahetseng ke senotlolo sa katleho ea teko;

Boima ba 'mele: ≥ 200mg lisele tse ncha;

Mali: ≥ 2 mL

Phallo ea Mosebetsi oa Ts'ebeletso

Mohlala oa QC

Moqapi oa liteko

tlhahiso ea mohlala

Mohlala oa thomello

Teko ea sefofane

Ho ntšoa ha DNA

Tokiso ea Laebrari

Kaho ea laebrari

Tatelano

Tatelano

Tlhahlobo ea lintlha

Tlhahlobo ea lintlha

Ka mor'a ho rekisa Litšebeletso

Litšebeletso tsa ka mor'a thekiso

  • E fetileng:
  • E 'ngoe:

    • 表观-02

    1.Heatmap ho ATAC e bala kabo ho TSS le libakeng tse haufi (± 3 kb)

    3 (1)2.Kabo ea sebaka se bulehileng sa chromatin likarolong tse fapaneng tsa genome

    4 (1)

    3.Differential tlhoro pitso pakeng tsa lihlopha

    5 (1)

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