Isku xigxiga genome bisulfite oo dhan
Faa'iidooyinka Adeegga
● Madal dhameystiran: bixi adeeg aad u wanaagsan oo hal joogsi ah laga bilaabo habaynta muunada, dhismaha maktabadda, isku xigxiga iyo falanqaynta bioinformatics.
● Saxnaanta sare: Tiknoolajiyada beddelka methylation ee qaan-gaadhka ah waxay si sax ah u falanqeyn kartaa heerka methylation ee hal saldhig C.
● Daboolista ballaaran: ogaanshaha goobaha methylation ee heerka genome-ballaaran.
● Ku celcelin wanaagsan: habayn deggan, ku habboon falanqaynta isbarbardhigga ee muunado badan.
Tilmaamaha Tusaalaha
|
Platform
|
Akhri Dhererka
|
Istaraatiijiyada Taxanaha
|
| Illumina | PE 150 | 30X, saddex nuqul oo bayooloji ah, oo leh genome tixraac oo tayo sare leh |
Shuruudaha Tusaalaha
|
Tusaalaha Nooca
|
Qadarka (μg)
|
Conc.(ng/μl)
|
Mugga (μl)
|
daahirnimo
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| Nabaad-guur iyo faddarayn xaddidan
|
Socodka Shaqada Adeegga
Tusaalaha keenista
Soo saarista DNA
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
gaarsiinta xogta
Bioinformatics
1.Gnome-ballaaran qaybinta methylation DNA
2.Anotation on aadka-methylated CGI gobolka
