Hufnaanta calaamadaynta sare- Tiknoolajiyada isku xigxiga ee sare-u-qaadka ah waxay ka caawisaa SLAF-Seq soo saarista boqollaal kun oo calaamado ah oo ku dhex jira genome-ga oo dhan.
Ku tiirsanaanta hoose ee genome- Waxaa lagu dabaqi karaa noocyada mid leh ama aan lahayn genome tixraac ah.
Naqshadaynta nidaamka dabacsan- Hal-enzyme, dual-enzyme, dheef-shiid kiimikaad badan iyo noocyo kala duwan oo enzymes ah, dhammaantood waa la dooran karaa si loo daboolo yoolalka cilmi-baarista ama noocyada kala duwan.Qiimaynta hore ee silico ayaa loo isticmaalaa si loo xaqiijiyo naqshadaynta enzyme ugu fiican.
Dheefshiidka enzymatic oo hufan- Tijaabo hore ayaa loo sameeyay si loo wanaajiyo xaaladaha, taas oo ka dhigaysa tijaabada rasmiga ah mid deggan oo la isku halayn karo.Hufnaanta ururinta jajabku waxay gaari kartaa in ka badan 95%.
Calaamadaha SLAF si siman loo qaybiyay- Calaamadaha SLAF waxay si siman ugu qaybsan yihiin dhammaan koromosoomyada illaa xadka ugu badan, iyagoo gaaraya celcelis ahaan 1 SLAF 4 kb.
Ka fogaanshaha waxtarka leh ee soo noqnoqdaIsku-xigta soo noqnoqda ee xogta SLAF-Seq ayaa hoos loo dhigay ilaa 5%, gaar ahaan noocyada leh heerka sare ee soo noqnoqda, sida sarreenka, galleyda, iwm.
Khibrad ballaadhan-In ka badan 2000 oo mashruuc SLAF-Seq ah oo xidhay boqolaal nooc oo daboolaya dhirta, naasleyda, shimbiraha, cayayaanka, noolaha-aqua-organisms, iwm.
Socod-shaqo bioinformatic ah oo iskeed u horumarisay- Socod-hawleed bioinformatic ah oo isku-dhafan oo loogu talagalay SLAF-Seq waxaa soo saaray BMKGENE si loo hubiyo isku halaynta iyo saxnaanta wax-soo-saarka kama-dambaysta ah.
Platform | Conc.(ng/gl) | Wadartaug) | OD260/280 |
Illumina NovaSeq | >35 | >1.6(Qaybta>15μl) | 1.6-2.5 |
Qoto dheer ee isku xigxiga: 10X/Tag
Cabirka Genome | Tags SLAF lagu taliyay |
< 500 Mb | 100k ama WGS |
500 Mb- 1 Gb | 100 K |
1 Gb -2 Gb | 200 K |
Giant ama genomes adag | 300 - 400K |
Codsiyada
| lagu taliyay Qiyaasta Dadweynaha
| Istaraatiijiyada isku xigxiga iyo qoto dheer
| |
Qoto dheer
| Lambarka Tag
| ||
GWAS
| Tusaalaha nambarka ≥ 200
| 10X
|
Sida laga soo xigtay cabbirka genome
|
Evolution Hidde
| Shakhsiyaadka mid kasta koox-hoosaad ≥ 10; muunado guud ≥ 30
| 10X
|
Weelka: 2 ml tuubo centrifuge
Inta badan shaybaarada, waxaan kugula talineynaa inaan lagu keydin ethanol.
Calaamadaynta Tusaalaha: Muunaduhu waxay u baahan yihiin in si cad loo calaamadiyo oo la mid ah foomka macluumaadka muunada ee la gudbiyay.
Shixnadda: Barafka qallalan: Muunadodu waxay u baahan yihiin in marka hore bac lagu shubo oo lagu aaso baraf qallalan.
1. Tirakoobka natiijada khariidada
2. Horumarinta calaamadaha SLAF
3. Faahfaahin kala duwan
Sannadka | Joornaal | IF | Ciwaanka | Codsiyada |
2022 | Isgaarsiinta dabeecadda | 17.694 | Asalkii genomic ee giga-chromosomes iyo giga-genome of peony geed Paeonia ostii | SAF-GWAS |
2015 | Cilmi-nafsiga cusub | 7.433 | Raad-raacyada gurigu waxay ku xidhaan gobollada genomic ee muhiimadda beeralayda gudaha digirta | SAF-GWAS |
2022 | Joornaalka Cilmi-baarista Sare | 12.822 | Genom-ballaaran hordhaca macmalka ah ee Gossypium barbadense galay G. hirsutum Muujin heer sare ah oo loogu talagalay hagaajinta tayada fiber-ka suufka ah iyo wax-soo-saarka sifooyinka | SLAF-horumarka hiddaha |
2019 | Warshada Molecular | 10.81 | Falanqaynta Genomic Dadweynaha iyo Golaha De Novo ayaa Muujiyay Asalka Weedy Bariiska sida Ciyaar Horumarineed | SLAF-horumarka hiddaha |
2019 | Hiddaha Dabeecada | 31.616 | Isku xigxiga genome iyo kala duwanaanshaha hidde-sidaha ee carp-ka caadiga ah, Cyprinus carpio | Khariidadda SLAF-Likage |
2014 | Hiddaha Dabeecada | 25.455 | Hiddo-sidaha lawska la beeray waxa uu siinaya aragtida legume karyotypes, polyploid kobcinta iyo dalagyada dalagyada. | Khariidadda SLAF-Likage |
2022 | Wargeyska Biotechnology ee dhirta | 9.803 | Aqoonsiga ST1 wuxuu muujinayaa xulashada ku lug leh garaacista qaab-dhismeedka abuurka iyo waxa ku jira saliidda inta lagu jiro dhaqashada soybean | Horumarinta SLF-Marker |
2022 | Joornaalka Caalamiga ah ee Cilmiga Molecular | 6.208 | Aqoonsiga iyo Horumarinta Calaamadaha DNA ee Qamadiga-Leymus mollis 2Ns (2D) Beddelka Kala-duwan ee Chromosome | Horumarinta SLF-Marker |