Alaabta

Isku-dabaynta hal-xuduneed ee RNA

Horumarka lagu sameeyay hal unug iyo farsamada dhismaha maktabadda gaarka ah oo lagu daray isku xigxiga-sare ee soo saarista waxay saamaxdaa daraasado muujinta hidde-sidaha oo ku salaysan unug-unug.Waxay awood u siisaa falanqayn qoto dheer oo dhamaystiran oo ku saabsan nidaamka unugyada isku dhafan, taas oo ay si weyn uga fogaato qarinta kala duwanaanshahooda iyada oo qaadashada celceliska dhammaan unugyada.

Si kastaba ha ahaatee, unugyada qaarkood kuma haboona in laga dhigo hal unug hal unug ah, markaa habab kale oo muunad diyaarin ah - soo saarista nukleus ee unugyada ayaa loo baahan yahay, taas oo ah, nukleus si toos ah ayaa looga soo saaraa unugyo ama unug waxaana loo diyaariyey hal-budada ka joojinta hal-hal- isku xigxiga unugga.

BMK waxa ay bixisaa 10x Genomics ChromiumTM ku salaysan adeegga isku xigxiga hal unug ee RNA.Adeeggan ayaa si weyn loogu isticmaalay daraasado ku saabsan cilmi-baadhisyo la xidhiidha cudurrada, sida kala-soocidda unugyada difaaca, kala duwanaanshaha burooyinka, horumarinta unugyada, iwm.

Chip transcriptome Spatial: 10× Genomics

Platform: Illumina NovaSeq Platform

Nala soo xiriir

Faahfaahinta Adeegga

Bioinformatics

Natiijooyinka Demo

Mabda'a farsamada

Go'doominta nuclei-yada waxaa lagu gaaraa 10 × Genomics Chromium TM, kaas oo ka kooban siddeed kanaal oo ah nidaamka microfluidics oo leh laba isgoys.Nidaamkan, boodhadhka jel ee leh barcodes iyo primer, enzymes iyo hal nucleus ayaa ku jira dhibicda saliidda ee nanoliter-ka, taasoo dhalinaysa Gel Bead-in-Emulsion (GEM).Marka GEM la sameeyo, lysis unug iyo sii deynta barcodes ayaa lagu sameeyaa GEM kasta.mRNA waxaa loo rogaa unugyo cDNA ah oo wata 10x barcodes iyo UMI, kuwaas oo ku sii xidhan dhismaha maktabadda isku xigxiga ee caadiga ah.

Unug aan ku habboonayn diyaarinta ka joojinta unugga keliya

Unug / unug	Sababta
Ka saar unug barafaysan	Aan awoodin in la helo ururo cusub ama muddo dheer la badbaadiyey
Unugga murqaha, Megakaryocyte, Dufan…	Dhexroorka unuggu aad buu u weyn yahay si aan loo gelin qalabka
Beerka…	Aad bay u jilicsan tahay in la jebiyo, oo aan awoodin in la kala saaro unugyada keliya
Unugga neerfaha, Maskaxda…	Aad u xasaasisan, oo ay fududahay in la caddeeyo, ayaa bedeli doonta natiijooyinka isku xigxiga
Pankres, Thyroid…	Qani ku ah enzymes endogenous, oo saameeya soo saarista joojinta hal unug

Hal-nukleus vs Single-unug

Hal-xudun	Hal unug
Dhexroor unug aan xadidnayn	Dhexroorka unugga: 10-40 μm
Maaddadu waxay noqon kartaa unug barafaysan	Walaxdu waa inay noqotaa unug cusub
Walwalka hoose ee unugyada barafaysan	Daawaynta enzyme waxay keeni kartaa dareen-celinta cadaadiska unugga
Looma baahna in unugyada dhiigga cas laga saaro	Unugyada dhiigga cas waxay u baahan yihiin in la saaro
Nukliyeerku wuxuu muujiyaa macluumaadka noolaha	Unugga oo dhan waxa uu muujiyaa macluumaadka noolaha