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Isku-dabaynta hal-xuduneed ee RNA
Horumarka lagu sameeyay hal unug iyo farsamada dhismaha maktabadda gaarka ah oo lagu daray isku xigxiga-sare ee soo saarista waxay saamaxdaa daraasado muujinta hidde-sidaha oo ku salaysan unug-unug.Waxay awood u siisaa falanqayn qoto dheer oo dhamaystiran oo ku saabsan nidaamka unugyada isku dhafan, taas oo ay si weyn uga fogaato qarinta kala duwanaanshahooda iyada oo qaadashada celceliska dhammaan unugyada.
Si kastaba ha ahaatee, unugyada qaarkood kuma haboona in laga dhigo hal unug hal unug ah, markaa habab kale oo muunad diyaarin ah - soo saarista nukleus ee unugyada ayaa loo baahan yahay, taas oo ah, nukleus si toos ah ayaa looga soo saaraa unugyo ama unug waxaana loo diyaariyey hal-budada ka joojinta hal-hal- isku xigxiga unugga.
BMK waxa ay bixisaa 10x Genomics ChromiumTM ku salaysan adeegga isku xigxiga hal unug ee RNA.Adeeggan ayaa si weyn loogu isticmaalay daraasado ku saabsan cilmi-baadhisyo la xidhiidha cudurrada, sida kala-soocidda unugyada difaaca, kala duwanaanshaha burooyinka, horumarinta unugyada, iwm.
Chip transcriptome Spatial: 10× Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
transcriptomics Spatial transcriptomics waxay u taagan tahay safka hore ee hal-abuurnimada sayniska, taasoo awood u siinaysa cilmi-baarayaasha inay dhexgalaan qaababka muujinta hidde-sidaha ee qallafsan ee unugyada dhexdooda iyagoo ilaalinaya xaaladdooda bannaan.Meelo kala duwan oo ay ku jiraan, BMKGene waxay soo saartay BMKManu S1000 Spatial Transcriptome Chip, iyada oo ku faanaysaxallinta la xoojiyayee 5µM, gaarey tirada unug hoosaadka, iyo kartidejinta xallinta heerar badan.Chip-ka S1000, oo leh ku dhawaad 2 milyan oo baro, ayaa ka shaqeeya microwells oo ay ku dahaaran yihiin kuul ay ku raran yihiin shaybaarro qabsi oo meel bannaan ah.Maktabad cDNA ah, oo ay ku hodmeen barcode-yada bannaan, ayaa laga soo diyaariyey chip-ka S1000 oo markii dambe lagu sii raacay madal Illumina NovaSeq.Isku darka shaybaarrada barcodeed ee meel bannaan iyo UMI-yada waxay hubinayaan saxnaanta iyo gaarnimada xogta la soo saaray.BMKManu S1000 chip sifadiisa gaarka ah waxay ku jirtaa kala duwanaanshaheeda, oo bixisa dejinta xallinta heerar badan oo si fiican loogu hagaajin karo unugyo iyo heerar faahfaahsan oo kala duwan.Laqabsigani wuxuu u dhigayaa chip-ka doorasho aad u wanaagsan oo loogu talagalay daraasadaha transcriptomics ee kala duwan, hubinta isku dhafka boosaska saxda ah oo leh buuq yar.
Isticmaalka BMKManu S1000 chip iyo teknoolojiyadda transcriptomics kale ee goobta, cilmi-baarayaashu waxay heli karaan faham wanaagsan oo ku saabsan abaabulka ballaaran ee unugyada iyo isdhexgalka molecular ee adag ee ka dhex dhaca unugyada, iyagoo siinaya fikrado qiimo leh oo ku saabsan hababka asaasiga ah ee hababka bayoolojiga ee qaybo badan oo kala duwan, oo ay ku jiraan oncology, neuroscience, bayoolaji korriinka, immunology iyo cilmiga dhirta.
Platform: BMKManu S1000 chip iyo Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
transcriptomics Spatial transcriptomics waa tignoolajiyada gees-goynta ah oo u oggolaanaysa cilmi-baarayaasha inay baaraan qaababka muujinta hidda-wadaha ee unugyada dhexdooda iyagoo ilaalinaya xaaladdooda bannaan.Mid ka mid ah madal awood leh oo ku taal boggan waa 10x Genomics Visium oo ay weheliso taxanaha Illumina.Mabda'a 10X Visium wuxuu dul saaran yahay jajab khaas ah oo leh meel laqabsado oo loo qoondeeyay halkaasoo qaybaha unugyada la dhigo.Goobtan la qabsashada waxa ay ka kooban tahay baro kood leh, oo mid walba u dhigma meel bannaan oo gaar ah oo ku dhex jirta unugyada.Unugyada RNA ee la qabtay ee ka yimid nudaha ayaa markaa lagu calaamadeeyay tilmaameyaal unugyo gaar ah (UMIs) inta lagu guda jiro habka qorista.Barcodeedkan iyo UMI-yada waxay awood u siinayaan khariidad meeleed sax ah iyo qiyaasida muujinta hidda-wadaha ee xallinta hal unug.Isku darka shaybaarrada barcodeed ee meel bannaan iyo UMI-yada waxay hubinayaan saxnaanta iyo gaarnimada xogta la soo saaray.Isticmaalka tignoolajiyadan Spatial Transcriptomics, cilmi-baarayaashu waxay heli karaan faham qoto dheer oo ku saabsan abaabulka ballaaran ee unugyada iyo isdhexgalka unugyada kakan ee ka dhex dhaca unugyada, iyagoo siinaya aragtiyo qiimo leh oo ku saabsan hababka asaasiga ah ee hababka bayoolojiga ee dhinacyo badan, oo ay ku jiraan oncology, neuroscience, bayoolaji korriinka, immunology. , iyo cilmiga dhirta.
Platform: 10X Genomics Visium iyo Illumina NovaSeq
