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Chromatin Taxanaha Tallaalka Tallaalka (ChiP-seq)
ChIP-Seq waxay bixisaa sifaynta genome-ballaaran ee bartilmaameedyada DNA ee wax ka beddelka histone, qodobbada qoraalka, iyo borotiinada kale ee DNA-da la xidhiidha.Waxay isku daraysaa xulashada chromatin immuno-precipitation (ChiP) si ay u soo kabsato dhismooyin borotiin-DNA gaar ah, oo leh awoodda isku xigxiga jiilka xiga (NGS) ee isku xigxiga-sare ee DNA-da la soo kabsaday.Intaa waxaa dheer, sababtoo ah iskudhafka borotiinka-DNA waxaa laga soo ceshaday unugyada nool, goobaha isku-xidhka waxaa lagu barbar dhigi karaa noocyada unugyada iyo unugyada kala duwan, ama xaalado kala duwan.Codsiyada waxay u dhexeeyaan nidaaminta qoraalka ilaa dariiqooyinka korriinka ilaa hababka cudurada iyo wixii ka baxsan.
Platform: Illumina NovaSeq Platform
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Taxanaha Metagenomic -NGS
Metagenome waxa loola jeedaa ururinta wadarta walxaha hidde-sidaha ee bulshada isku dhafan ee noolaha, sida metagenome deegaanka, metagenome-ka bini'aadamka, iwm. Waxa uu ka kooban yahay hiddo-sideyaasha noolaha la beeran karo iyo kuwa aan la beeran karin labadaba.Isku xigxiga Metagenomic waa qalab molecular loo isticmaalo in lagu falanqeeyo walxaha hidde-sideyaasha isku dhafan ee laga soo saaro muunado deegaanka, kaas oo siinaya macluumaad faahfaahsan oo ku saabsan kala duwanaanshaha noocyada iyo badnaanta, qaab dhismeedka dadweynaha, xiriirka phylogenetic, hiddo-wadaha shaqeynta iyo isku-xirnaanta arrimaha deegaanka.
Platform:Illumina NovaSeq Platform
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Taxanaha Metagenomic-Nanopore
Metagenomics waa qalab molecular oo loo isticmaalo in lagu falanqeeyo walxaha isku dhafan ee genomic ee laga soo saaro shaybaarada deegaanka, kaas oo siinaya macluumaad faahfaahsan oo ku saabsan kala duwanaanta iyo badnaanta noocyada, qaab dhismeedka dadweynaha, xiriirka phylogenetic, hiddo-wadaha shaqeynaya iyo isku-xirnaanta arrimaha deegaanka, iwm barashada metagenomic.Wax qabadkeeda aadka u wanaagsan ee dhererka akhrinta ayaa si weyn kor ugu qaaday falanqaynta metagenomic-ga qulqulka, gaar ahaan isu-ururinta metagenome.Qaadashada faa'iidooyinka dhererka-akhriska, cilmi-baadhis metagenomic ku salaysan ee Nanopore ayaa awood u leh in ay gaadho kulan joogto ah oo dheeraad ah marka la barbar dhigo metagenomics-ka-tooska ah.Waxaa la daabacay in Metagenomics-ku-saleysan Nanopore ay si guul leh u soo saareen genomes bakteeriyada dhamaystiran oo xiran oo ka yimid microbiomes (Moss, EL, et. al,Biotech dabiiciga ah, 2020)
Platform:Nanopore PromethION P48
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Isku xigxiga genome bisulfite oo dhan
Methylation DNA ee booska shanaad ee cytosine (5-mC) ayaa saameyn aasaasi ah ku leh muujinta hiddaha iyo dhaqdhaqaaqa gacanta.Qaababka methylation-ka aan caadiga ahayn ayaa lala xiriiriyay xaalado iyo cuduro dhowr ah, sida kansarka.WGBS waxay noqotay halbeegga dahabiga ah ee lagu baranayo methylation-ka-ballaaran ee genome-ga ee xallinta hal saldhig.
Platform: Illumina NovaSeq Platform
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Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo
ATAC-seq waa hab isku xigxig heersare ah oo lagu falanqeeyo gelitaanka chromatin-ka genom-ballaaran, taas oo muhiim u ah xakamaynta epigenetic-ka caalamiga ah ee muujinta hidda-wadaha.Adabiyeyaasha isku xigxiga ayaa la geliyo gobollada chromatin ee furan iyadoo loo marayo transposase firfircoon oo Tn5 ah.Kordhinta PCR ka dib, maktabad taxane ah ayaa la dhisay.Dhammaan gobollada chromatin-ka furan waxaa lagu heli karaa xaalad gaar ah oo waqti-space ah, oo aan ku xaddidnayn oo keliya goobaha lagu xirayo qodobka qoraalka, ama gobol gaar ah oo histone wax laga beddelay.
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16S/18S/ITS Amplicon Sequencing-PacBio
Qaybta hoose ee 16S iyo 18S rRNA oo ka kooban labada gobol ee aadka loo ilaaliyo iyo kuwa isbedbeddela leh ayaa ah faraha molikula ee ugu fiican ee aqoonsiga noolaha prokaryotic iyo eukaryotic.Ka faa'iidaysiga isku xigxiga, amplicons-yadan waxaa lagu bartilmaameedsan karaa iyadoo lagu salaynayo qaybaha la xafiday iyo gobollada kala duwanaanshiyaha ayaa si buuxda loogu sifeyn karaa aqoonsiga microbial ee gacan ka geysanaya daraasadaha daboolaya falanqaynta kala duwanaanta microbial, taxonomy, phylogeny, iwm. Hal-molecule-waqtiga dhabta ah (SMRT) Isku xigxiga madal PacBio waxa ay sahlaysa in la helo akhrin dheer oo sax ah, kaas oo dabooli kara amplicons dhererkiisu dhan yahay (qiyaastii 1.5 Kb).Aragtida ballaaran ee beerta hidde-sidaha ayaa si weyn u xoojisay xallinta sharraxaadda noocyada bakteeriyada ama fangaska bulshada.
Platform:Talaabada PacBio II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS isku xigxiga amplicon waxay ujeedadeedu tahay muujinta phylogeny, taxonomy, iyo noocyada badan ee bulshada microbial iyadoo la baarayo alaabta PCR ee calaamadaha hidda-waynta ee ka kooban qaybaha aadka loo wada sheekaysanayo iyo kuwa kala duwan.Soo bandhigida faraha molecular ee kaamil ah ee Woeses et al, (1977) waxay awood u siisaa sifaynta microbiome-ka-freeIsku xigxiga 16S (bakteeriyada), 18S (Fungi) iyo Internal transcribed spacer (ITS, fungi) waxay ogolaataa aqoonsiga noocyada badan ee noocyada kala duwan iyo sidoo kale noocyada dhifka ah iyo kuwa aan la aqoonsan.Tiknoolajiyadani waxay noqotay qalab si ballaaran loo isticmaalo oo lagu garto ka kooban microbial kala duwan ee deegaan kala duwan, sida afka aadanaha, xiidmaha, saxarada, iwm.
Platform:Illumina NovaSeq Platform
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Dib-u-habaynta Bakteeriyada iyo Fungal Genom Genome
Bakteeriyada iyo fangasyada dib-u-soo-celinta genome-ga oo dhan waa qalab muhiim ah oo lagu dhammaystiro genome-yada bakteeriyada iyo fangaska la yaqaan, iyo sidoo kale in la is barbar dhigo genome badan ama in la sawiro genomes-ka noole cusub.Aad bay muhiim u tahay in la isku dayo dhammaan genome-yada bakteeriyada iyo fangaska si loo soo saaro genome tixraac sax ah, si loo sameeyo aqoonsiga microbial iyo daraasado kale oo genome is barbar dhig ah.
Platform:Illumina NovaSeq Platform
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PacBio-dhererka-buuxa 16S/18S/ITS Taxanaha Amplicon
Amplicon (16S / 18S / ITS) madal waxaa lagu horumariyaa iyadoo sano oo waayo-aragnimo ah ee falanqaynta mashruuca kala duwanaansho microbial, kaas oo ka kooban falanqaynta aasaasiga ah ee caadiga ah iyo falanqaynta shakhsiyeed: falanqaynta aasaasiga ah daboolayaa content falanqaynta caadiga ah ee cilmi microbial hadda, content falanqaynta waa qani iyo dhamaystiran. iyo natiijooyinka falanqaynta waxaa lagu soo bandhigay qaab warbixin mashruuc;Nuxurka falanqaynta shakhsi ahaaneed waa kala duwan.Tusaalooyinka waa la dooran karaa waxaana loo dejin karaa si dabacsan iyadoo loo eegayo warbixinta falanqaynta aasaasiga ah iyo ujeedada cilmi-baarista, si loo xaqiijiyo shuruudaha shakhsi ahaaneed.Nidaamka hawlgalka Windows, fudud oo degdeg ah.
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PacBio-Full-dherer Transcriptome (Tixraac la'aan)
Qaadashada xogta isku xigxiga ee Baasifiga (PacBio) Isoform sida galinta, App-kan waxa uu awoodaa in uu aqoonsado taxanaha qoraalka qoraalka dhererka buuxa (la'aan la kulmin).Marka la sawiro taxanaha dhererka buuxa ee lidka ku ah genome tixraaca, qoraalada waxaa lagu hagaajin karaa hiddo-wadaha la yaqaan, qoraallada qoraalka, gobollada codeynta, iwm. Xaaladdan, aqoonsi sax ah oo ka badan qaab-dhismeedka mRNA, sida kala-bax kale, iwm, ayaa la gaari karaa.Falanqaynta wadajirka ah ee xogta isku xigxiga qoraalka ee NGS waxay awood u siinaysaa sharraxaad dhammaystiran iyo tiraab sax ah oo muujinta heerka qoraalka, taas oo inta badan faa'iido u leh muujinta kala duwanaanta hoose iyo falanqaynta shaqeynta.
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Taxanaha Bisulfite ee la dhimay (RRBS)
Cilmi-baarista methylation DNA waxay had iyo jeer ahayd mawduuc kulul cilmi-baarista cudurrada, waxayna si dhow ula xiriirtaa muujinta hidda-wadaha iyo sifooyinka pheno-nooca.RRBS waa hab sax ah, hufan oo dhaqaale oo loogu talagalay cilmi-baarista methylation DNA.Kobcinta dhiirrigeliyeyaasha iyo gobollada jasiiradaha CpG iyadoo la kala gooyo enzymatic (Msp I), oo ay weheliso taxanaha Bisulfite, waxay bixisaa ogaanshaha methylation DNA xallin sare.
Platform: Illumina NovaSeq Platform
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Taxanaha prokaryotic mRNA
Taxanaha mRNA waxa uu awood siinayaa sifada dhamaystiran ee dhamaan qoraalada mRNA-ga ee unugyada ku jira xaalado gaar ah.Tiknoolajiyadan casriga ah waxay u adeegtaa sidii qalab awood leh, oo daaha ka qaadaysa muuqaalada muujinta hidda-wadaha, qaab-dhismeedka hidda-wadaha, iyo hababka molecular ee la xidhiidha hababka noole ee kala duwan.Si ballaaran loo qaatay cilmi baarista aasaasiga ah, baarista caafimaadka, iyo horumarinta daroogada, isku xigxiga mRNA waxa uu bixiyaa aragtiyo ku saabsan qalafsanaanta dhaqdhaqaaqa gacanta iyo habaynta hidda-socodka.Samaynta muunadda prokaryotic mRNA ee prokaryotic waxaa loogu talagalay qoraallada prokaryotic, ee ku lug leh dhimista rRNA iyo diyaarinta maktabad jihaynta.
Platform: Illumina NovaSeq X
