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Taxanaha Metagenomic -NGS
Metagenome waxa loola jeedaa ururinta wadarta walxaha hidde-sidaha ee bulshada isku dhafan ee noolaha, sida metagenome deegaanka, metagenome-ka bini'aadamka, iwm. Waxa uu ka kooban yahay hiddo-sideyaasha noolaha la beeran karo iyo kuwa aan la beeran karin labadaba.Isku xigxiga Metagenomic waa qalab molecular loo isticmaalo in lagu falanqeeyo walxaha hidde-sideyaasha isku dhafan ee laga soo saaro muunado deegaanka, kaas oo siinaya macluumaad faahfaahsan oo ku saabsan kala duwanaanshaha noocyada iyo badnaanta, qaab dhismeedka dadweynaha, xiriirka phylogenetic, hiddo-wadaha shaqeynta iyo isku-xirnaanta arrimaha deegaanka.
Platform:Illumina NovaSeq Platform
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Taxanaha Metagenomic-Nanopore
Metagenomics waa qalab molecular oo loo isticmaalo in lagu falanqeeyo walxaha isku dhafan ee genomic ee laga soo saaro shaybaarada deegaanka, kaas oo siinaya macluumaad faahfaahsan oo ku saabsan kala duwanaanta iyo badnaanta noocyada, qaab dhismeedka dadweynaha, xiriirka phylogenetic, hiddo-wadaha shaqeynaya iyo isku-xirnaanta arrimaha deegaanka, iwm barashada metagenomic.Wax qabadkeeda aadka u wanaagsan ee dhererka akhrinta ayaa si weyn kor ugu qaaday falanqaynta metagenomic-ga qulqulka, gaar ahaan isu-ururinta metagenome.Qaadashada faa'iidooyinka dhererka-akhriska, cilmi-baadhis metagenomic ku salaysan ee Nanopore ayaa awood u leh in ay gaadho kulan joogto ah oo dheeraad ah marka la barbar dhigo metagenomics-ka-tooska ah.Waxaa la daabacay in Metagenomics-ku-saleysan Nanopore ay si guul leh u soo saareen genomes bakteeriyada dhamaystiran oo xiran oo ka yimid microbiomes (Moss, EL, et. al,Biotech dabiiciga ah, 2020)
Platform:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Qaybta hoose ee 16S iyo 18S rRNA oo ka kooban labada gobol ee aadka loo ilaaliyo iyo kuwa isbedbeddela leh ayaa ah faraha molikula ee ugu fiican ee aqoonsiga noolaha prokaryotic iyo eukaryotic.Ka faa'iidaysiga isku xigxiga, amplicons-yadan waxaa lagu bartilmaameedsan karaa iyadoo lagu salaynayo qaybaha la xafiday iyo gobollada kala duwanaanshiyaha ayaa si buuxda loogu sifeyn karaa aqoonsiga microbial ee gacan ka geysanaya daraasadaha daboolaya falanqaynta kala duwanaanta microbial, taxonomy, phylogeny, iwm. Hal-molecule-waqtiga dhabta ah (SMRT) Isku xigxiga madal PacBio waxa ay sahlaysa in la helo akhrin dheer oo sax ah, kaas oo dabooli kara amplicons dhererkiisu dhan yahay (qiyaastii 1.5 Kb).Aragtida ballaaran ee beerta hidde-sidaha ayaa si weyn u xoojisay xallinta sharraxaadda noocyada bakteeriyada ama fangaska bulshada.
Platform:Talaabada PacBio II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS isku xigxiga amplicon waxay ujeedadeedu tahay muujinta phylogeny, taxonomy, iyo noocyada badan ee bulshada microbial iyadoo la baarayo alaabta PCR ee calaamadaha hidda-waynta ee ka kooban qaybaha aadka loo wada sheekaysanayo iyo kuwa kala duwan.Soo bandhigida faraha molecular ee kaamil ah ee Woeses et al, (1977) waxay awood u siisaa sifaynta microbiome-ka-freeIsku xigxiga 16S (bakteeriyada), 18S (Fungi) iyo Internal transcribed spacer (ITS, fungi) waxay ogolaataa aqoonsiga noocyada badan ee noocyada kala duwan iyo sidoo kale noocyada dhifka ah iyo kuwa aan la aqoonsan.Tiknoolajiyadani waxay noqotay qalab si ballaaran loo isticmaalo oo lagu garto ka kooban microbial kala duwan ee deegaan kala duwan, sida afka aadanaha, xiidmaha, saxarada, iwm.
Platform:Illumina NovaSeq Platform
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Dib-u-habaynta Bakteeriyada iyo Fungal Genom Genome
Bakteeriyada iyo fangasyada dib-u-soo-celinta genome-ga oo dhan waa qalab muhiim ah oo lagu dhammaystiro genome-yada bakteeriyada iyo fangaska la yaqaan, iyo sidoo kale in la is barbar dhigo genome badan ama in la sawiro genomes-ka noole cusub.Aad bay muhiim u tahay in la isku dayo dhammaan genome-yada bakteeriyada iyo fangaska si loo soo saaro genome tixraac sax ah, si loo sameeyo aqoonsiga microbial iyo daraasado kale oo genome is barbar dhig ah.
Platform:Illumina NovaSeq Platform
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Taxanaha Metatranscriptome
Isku xigxiga Metatranscriptome wuxuu tilmaamayaa muujinta hidda-wadaha ee microbes (labada eukaryotes iyo prokaryotes) gudaha deegaanka dabiiciga ah (sida ciidda, biyaha, badda, saxarada, iyo mindhicirka.) noocyada, falanqaynta kobcinta functional ee hiddo-sidaha si kala duwan loo muujiyey, iyo in ka badan.
Platform: Illumina NovaSeq Platform
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Fungal Genome
Tiknoolajiyada Biomarker waxay bixiyaan sahan genome, genome ganaax ah iyo genome-dhameystiran genome ee fangas iyadoo ku xiran hadafka cilmi baarista gaarka ah.Taxanaha genome-ga, isu-ururinta iyo sharraxaadda shaqada waxa lagu gaadhi karaa iyadoo la isku darayo taxanaha jiilka xiga + taxanaha jiilka saddexaad si loo gaadho isu-ururinta genome-ga heerka sare ah.Tignoolajiyada Hi-C sidoo kale waxaa loo adeegsan karaa si loo fududeeyo isu-ururinta genome-ga heerka koromosoomyada.
Platform:Talaabada PacBio II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Bakteeriyada Genome oo Dhamaystiran
Teknolojiyadda Biomarker waxay bixisaa adeeg isku xigxig ah oo lagu dhisayo genome-ka bakteeriyada oo dhammaystiran oo aan lahayn farqi eber ah.Shaqada ugu muhiimsan ee bakteeriyadu waxay dhamaystiraysaa dhismaha genome-ga waxaa ka mid ah isku xigxiga jiilka saddexaad, isu-ururinta, sharraxaadda shaqeynta iyo falanqaynta bioinformatic-ka sare ee buuxinaysa yoolalka cilmi-baarista gaarka ah.Bakteeriyada genome-ka ee genome-ka ayaa awood u siinaya muujinta hababka aasaasiga ah ee ka hooseeya hababka bayoolojiga, taas oo sidoo kale siin karta tixraac qiimo leh cilmi-baarisyada genomic ee noocyada sare ee eukaryotic.
Platform:Nanopore PromethION P48 + Illumina NovaSeq Platform
Talaabada PacBio II
