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Chromatin Taxanaha Tallaalka Tallaalka (ChiP-seq)
ChIP-Seq waxay bixisaa sifaynta genome-ballaaran ee bartilmaameedyada DNA ee wax ka beddelka histone, qodobbada qoraalka, iyo borotiinada kale ee DNA-da la xidhiidha.Waxay isku daraysaa xulashada chromatin immuno-precipitation (ChiP) si ay u soo kabsato dhismooyin borotiin-DNA gaar ah, oo leh awoodda isku xigxiga jiilka xiga (NGS) ee isku xigxiga-sare ee DNA-da la soo kabsaday.Intaa waxaa dheer, sababtoo ah iskudhafka borotiinka-DNA waxaa laga soo ceshaday unugyada nool, goobaha isku-xidhka waxaa lagu barbar dhigi karaa noocyada unugyada iyo unugyada kala duwan, ama xaalado kala duwan.Codsiyada waxay u dhexeeyaan nidaaminta qoraalka ilaa dariiqooyinka korriinka ilaa hababka cudurada iyo wixii ka baxsan.
Platform: Illumina NovaSeq Platform
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Isku xigxiga genome bisulfite oo dhan
Methylation DNA ee booska shanaad ee cytosine (5-mC) ayaa saameyn aasaasi ah ku leh muujinta hiddaha iyo dhaqdhaqaaqa gacanta.Qaababka methylation-ka aan caadiga ahayn ayaa lala xiriiriyay xaalado iyo cuduro dhowr ah, sida kansarka.WGBS waxay noqotay halbeegga dahabiga ah ee lagu baranayo methylation-ka-ballaaran ee genome-ga ee xallinta hal saldhig.
Platform: Illumina NovaSeq Platform
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Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo
ATAC-seq waa hab isku xigxig heersare ah oo lagu falanqeeyo gelitaanka chromatin-ka genom-ballaaran, taas oo muhiim u ah xakamaynta epigenetic-ka caalamiga ah ee muujinta hidda-wadaha.Adabiyeyaasha isku xigxiga ayaa la geliyo gobollada chromatin ee furan iyadoo loo marayo transposase firfircoon oo Tn5 ah.Kordhinta PCR ka dib, maktabad taxane ah ayaa la dhisay.Dhammaan gobollada chromatin-ka furan waxaa lagu heli karaa xaalad gaar ah oo waqti-space ah, oo aan ku xaddidnayn oo keliya goobaha lagu xirayo qodobka qoraalka, ama gobol gaar ah oo histone wax laga beddelay.
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Taxanaha Bisulfite ee la dhimay (RRBS)
Cilmi-baarista methylation DNA waxay had iyo jeer ahayd mawduuc kulul cilmi-baarista cudurrada, waxayna si dhow ula xiriirtaa muujinta hidda-wadaha iyo sifooyinka pheno-nooca.RRBS waa hab sax ah, hufan oo dhaqaale oo loogu talagalay cilmi-baarista methylation DNA.Kobcinta dhiirrigeliyeyaasha iyo gobollada jasiiradaha CpG iyadoo la kala gooyo enzymatic (Msp I), oo ay weheliso taxanaha Bisulfite, waxay bixisaa ogaanshaha methylation DNA xallin sare.
Platform: Illumina NovaSeq Platform
