MICROBIAL
Waafaqsanaanta bakteeriyada iyo dhaqdhaqaaq la'aanta biochar waxay wanaajisay xaalufka tebuconazole, ka kooban microbiome ciidda iyo shaqeynta
Dhererka buuxa 16S isku xigxiga amplicon |PacBio HiFi |Kala duwanaanta Alpha |Kala duwanaanta beta
Daraasaddan, dhererka buuxa ee 16S amplicon sequencing by PacBio iyo falanqaynta bioinformatic waxaa bixiyay Biomarker Technologies.
Tilmaamaha
Biochar immobilized tebuconazole-bakteeriyada wax u dhimaysa Alcaligenes faecalis WZ-2 waxaa lagu daraaseeyay waxtarka biodegradation waxayna saamaysaa ciidda wasakhaysan ee tebuconazole marka loo eego cadaadiska WZ-2 ee bilaashka ah.
1. Biochar-immobilized WZ-2 waxay muujisay hoos u dhac waxtar leh oo tebuconazole ah marka la barbar dhigo WZ-2 bilaash ah iyada oo la dhimay tebuconazole nuska nolosha ciidda laga bilaabo 18.7 maalmood ilaa 13.3 maalmood.
2. Biochar-immobilized WZ-2 waxay awooday inay dib u soo celiso waxqabadyada enzyme microbial ee ciidda hooyo, oo ay ku jiraan urease, fuuq-baxa iyo leexinta, iwm.
3. profile microbial in biochar-immobilized WZ-2 daaweeyay ciidda lagu go'aamiyay dherer buuxa 16S isku xigxiga si xoog leh u taageeray in nidaamkani soo celin karo caafimaadka ciidda by hagaajinta qaab-dhismeedka bulshada bakteeriyada hoos wasakhda tebuconazole.
Tijaabo (Taxane la xidhiidha)
Kooxaynta: CK: Ciidda dabiiciga ah;T: Ciidda lagu saydhay tebuconazole;S: Tebuconazole waxaa ku jira carro leh cadaadis bilaash ah WZ-2;BC: Tebuconazole waxaa ku jira ciid leh biochar;BCS: Tebuconazole waxaa ku jira carro leh biochar aan dhaqdhaqaaq lahayn WZ-2.
Muunad: Wadarta soo saarista DNA-ga ciidda waxaa lagu xoojiyey 16S rDNA aasaasiga ah
27F (5′-AGAGTTTGATCCTGGCTCAG-3′) iyo 1492R (5′-GGTTACCTTGTACGA),soo celinaysa dhererka buuxa ee 16S rDNA
Habka isku xigxiga: PacBio RS II
Istaraatiijiyada isku xigxiga: CCS HIFI wuu akhriyaa
Falanqaynta xogta:BMKCloudBioinformatic Platforming ayaa kalluunka dahabka ka dhigaya nidaam moodal hidde ah oo heer sare ah oo loogu talagalay physiology kalluunka iyo kobaca.
Natiijo
Qaab dhismeedka beesha microbial ciidda waxaa lagu go'aamiyay isku xigxiga 16S rDNA.Qaninimada OTU iyo tusmada kala duwanaanta alfa, oo ay ku jiraan Chao1, Ace, Shannon iyo Simpson indices ayaa la qiimeeyay si loo muujiyo kala duwanaanshaha noocyada ee nidaam kasta.Ka dib 60 maalmood ka dib, dhammaan tusmooyinka ayaa muujiyay isbeddel la mid ah, tusaale ahaan tebuconazole waxay keeni kartaa hoos u dhaca hodantinimada noocyada iyo kala duwanaanta ciidda.Si kastaba ha ahaatee, iyada oo lagu daray cadaadis WZ-2, beesha bakteeriyada ciidda ayaa qayb ahaan laga soo kabsaday marka loo eego qaninimada iyo kala duwanaanta labadaba.Farqi xaddidan ayaa lagu arkay BC, BCS iyo CK, taasoo muujinaysa in biochar iyo biochar-immobilized WZ-2 ay si wax ku ool ah u soo celin karaan caafimaadka noolaha ee ciidda.
Habka kooxda-kooxeed ee aan miisaan lahayn oo leh macnaha Arithmetic (UPGMA) ayaa lagu dabaqay daraasaddan si loo muujiyo kala duwanaanshaha beta ee kooxaha.Sida ku cad shaxanka soo socda, BC, BSC iyo CK waxay wadaagaan qaab isku mid ah oo la mid ah halabuurka microbial marka la barbar dhigo kooxda T iyo S, taas oo sii muujisay in hordhaca biochar ee tebuconazole-wasakhaysan bioremediation ciidda ay si weyn u fududayn karto soo kabashada bulshada microbial ee ciidda.
SawirkaUPGMA UPGMA ee bulshada bakteeriyada heerka phylum ee daaweynta kala duwan
Tixraac
Sun, Tong, iyo al."Isku waafaqsanaanta bakteeriyada iyo dhaqdhaqaaqa ka saarista biochar waxay wanaajisay xaalufka tebuconazole, ka kooban microbiome ciidda iyo shaqeynta."Joornaalka Walxaha halista ah398 (2020): 122941.
Warar iyo Qodobo Muhiim ah ujeedadeedu tahay in la wadaago kiisaskii ugu dambeeyay ee lagu guulaystey Teknolojiyadda Biomarker, qabashada guulaha sayniska ee cusub iyo sidoo kale farsamooyinka caanka ah ee lagu dabaqay daraasadda.
Waqtiga boostada: Jan-08-2022