Wararka

Kordhinta curiyaha la beddeli karo (TE) waxaa loo aqoonsaday inuu yahay awood dhexdhexaadinaysa ballaadhinta cabbirka genome-ga iyo horumarka, laakiin cawaaqibka qaabaynta qaab-dhismeedka genomic 3D ayaa ah mid aan si weyn looga aqoon dhirta.Halkan, waxaanu ka warbixinaynaa shirarka genome-fasalka tixraaca ee saddex nooc oo cudbi ah oo u dhexeeya saddex laab oo cabbirka genome, kuwaas oo kala ah.Gossypium rotundifolium(K2),G. arboreum(A2), iyoG. raimondii(D5), iyadoo la isticmaalayo Oxford Nanopore Technologies.Falanqaynta genome-ka isbarbardhigga ahi waxay diiwaangelisaa faahfaahinta nasabka-gaarka ah ee TE-weyneynta ka qaybqaata kala duwanaanshaha cabbirka genome-ga weyn (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), oo waxay muujisaa nuxurka hidde-sidaha ilaa xad la xafiday iyo xidhiidhada isku dhafan ee genome-yada.Waxaan ogaanay in qiyaastii 17% hiddo-wadaha syntenic ay muujiyaan isbeddelka heerka chromatin ee u dhexeeya qaybaha firfircoon ("A") iyo kuwa aan firfircoonayn ("B"), iyo kordhinta TE waxay la xiriirtay korodhka saamiga qaybta A ee gobollada hiddaha (~ 7,000 hiddo-wadaha). ) ee K2 iyo A2 marka loo eego D5.Keliya 42% ee xudduudaha laxidhiidha topologically associating domain (TAD) ayaa lagu xafiday saddexda genom.Xogtayadu waxay khusaysaa kordhinta TE-yada ee dhowaan ka dib samaynta soohdimaha TAD ee u gaarka ah.Daraasadani waxay iftiiminaysaa doorka balaadhinta genome-ka dhexdhexaadinta transposon-ka ee horumarka qaab dhismeedka chromatin-ka sare ee dhirta.