Genotyping-sare-soo-saarka, gaar ahaan dadka tirada badan, waa tallaabo aasaasi ah ee daraasadaha ururka hidde, kaas oo bixiya aasaaska hidde-helka ee daahfurka hiddo-wadaha, falanqaynta korriinka, iwm. ) waxaa loo soo bandhigay si loo yareeyo qiimaha isku xigxiga ee muunad kasta, iyadoo la ilaalinayo waxtarka macquulka ah ee helitaanka calaamadeeyaha hidde-sideyaasha.Tan waxaa badanaa lagu gaaraa in la soo saaro jajab xaddidan oo ku dhex jira cabbirka cabbirka, kaas oo lagu magacaabo maktabadda matalaadda la dhimay (RRL).Taxanaha goos gooska gaarka ah ee meel-joogta ah (SLAF-Seq) waa istaraatiijiyad iskeed u horumarisay oo loogu talagalay de novo SNP daahfurka iyo SNP genotyping ee dad badan.
Socodka shaqada farsamada
SLAF vs hababka RRL ee jira
Faa'iidooyinka SLAF
Hufnaanta calaamadaynta hidde-sidaha sare- Marka lagu daro tikniyoolajiyadda isku xigxiga ee sare-sare, SLAF-Seq waxay gaari kartaa boqolaal kun oo tags ah oo laga helay genome-ga oo dhan si loo fuliyo codsiga mashaariicda cilmi-baarista ee kala duwan, ha ahaato ama leh genome tixraac ah.
Naqshad tijaabo ah oo la habeeyey& dabacsanaan leh- Hadafka cilmi-baarista ee kala duwan ama noocyada, xeeladaha dheef-shiid kiimikaadka enzymatic ee kala duwan ayaa diyaar ah oo ay ku jiraan hal-enzyme, dual-enzyme iyo dheef-shiid kiimikaad badan.Istaraatiijiyada dheefshiidka ayaa horay loogu qiimeyn doonaa silico si loo xaqiijiyo naqshadeynta enzyme ugu fiican.
Waxtarka sare ee dheefshiidka enzymatic- Dheef-shiid kiimikaad ee hore loo qorsheeyay wuxuu bixiyaa SLAF-yada si siman u qaybsan koromosoomyada.Uruurinta jajabka si hufan waxay gaari kartaa in ka badan 95%.
Iska ilaali isku xigxiga soo noqnoqda- Boqolkiiba isku xigxiga soo noqnoqda ee xogta SLAF-Seq ayaa hoos loo dhigay ilaa 5%, gaar ahaan noocyada leh heerka sare ee walxaha soo noqnoqda, sida sarreenka, galleyda, iwm.
Socod-shaqo bioinformatic ah oo iskeed u horumarisay- BMK waxay horumarisay qulqulka shaqada bioinformatic isku dhafan ee lagu dabaqi karo tignoolajiyada SLAF-Seq si loo hubiyo isku halaynta iyo saxnaanta wax soo saarka kama dambaysta ah.
Codsiga SLAF
Khariidadda isku xirka hiddaha
Dhismaha khariidad hidde cufan oo cufan iyo aqoonsiga loci xakamaynta sifooyinka nooca ubaxa ee Chrysanthemum (Chrysanthemum x morifolium Ramat.)
Joornaalka: Cilmi-baarista Beeraha La daabacay: 2020.7
GWAS
Aqoonsiga hidda-wadaha murashaxa ee la xidhiidha maaddada isofavone ee ku jirta abuurka soybeanka iyadoo la adeegsanayo ururka genome-ballaaran iyo khariidaynta isku xidhka
Joornaalka: Wargeyska Dhirta La daabacay: 2020.08
Hiddaha Kobcinta
Falanqaynta genomic ee dadweynaha iyo golaha de novo waxay muujinayaan asalka bariiska haramaha sida ciyaar horumar leh
Joornaalka: Dhirta Molecular La daabacay: 2019.5
Falanqaynta Qaybaha Badan (BSA)
GmST1, kaas oo dejinaya sulfotransferase, waxay siisaa iska caabbinta fayraska mosaic soybean G2 iyo G3
Joornaalka: Dhirta, Unugyada & Deegaanka La daabacay: 2021.04
Tixraac
Sun X, Liu D, Zhang X, iyo al.SLAF-Seq: hab wax ku ool ah oo baaxad weyn de novo SNP daahfurka iyo genotyping iyadoo la isticmaalayo isku xigxiga sare-throughput[J].Plos kow, 2013, 8 (3): e58700
Heesta X, Xu Y, Gao K, iyo al.Dhismaha khariidad hidde-cufnaanta sare leh iyo aqoonsiga loci xakamaynta sifooyinka nooca ubaxa ee Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.2020;7:108.
Wu D, Li D, Zhao X, iyo al.Aqoonsiga hidda-wadaha murashaxa ee la xidhiidha maaddada isoflavone ee ku jirta miraha soybean iyadoo la adeegsanayo ururka genome-ballaaran iyo khariidaynta isku xidhka.Dhirta J. 2020;104 (4): 950-963.
Sun J, Ma D, Tang L, iyo al.Falanqaynta Genomic Dadweynaha iyo Golaha De Novo ayaa Muujiyay Asalka Bariiska Weedy sida Ciyaar Horumarineed.Mol Plant.2019;12 (5):632-647.Mol Plant.2018;11 (11): 1360-1376.
Zhao X, Jing Y, Luo Z, iyo al.GmST1, kaas oo soo koobaya sulfotransferase, waxa ay siisaa iska caabbinta fayraska mosaic soybean G2 iyo G3.Deegaanka Unugga Dhirta.2021;10.1111/pce.14066
Waqtiga boostada: Jan-04-2022