ECOOLOGY MICROBIAL
Xoojinta beeruhu waxay yaraynaysaa kakanaanta shabakada microbial iyo tirada badan ee canshuurta furaha ee xididdada
Isku xigxiga Amplicon dhererka buuxa (ITS) |PacBio HIFI
Tilmaamaha
AXoojinta beeruhu waxa ay noqotey mid sii kordheysa dhibaatada ay sabab u tahay waxyeelooyinka deegaanka ee xun oo ay ka mid yihiin waxtarka isticmaalka nafaqeynta oo liidata, biyo-baxa goundwater, xaalufka tayada carrada, iwm. Nidaamyada beeralayda ee beddelka ah oo ay ku jiraan beeralayda aan-till iyo organic ayaa si weyn loo qaatay si loo yareeyo waxyeelada.Bulshada microbial waxay door lama huraan ah ka ciyaartaa wax soo saarka iyo waaritaanka nidaamka beeralayda.Si kastaba ha ahaatee, ma cadda in habka beerashada ee kala duwan ay u saameeyaan microbiota xididka.
Naqshadaynta Tijaabada
Tijaabooyin
SSaliida iyo xididka (DNA) ayaa laga soo qaaday beero sarreen ah oo laga keenay 60 dhul-beereed (20 midkiiba)
Grouping: 1. Convention (oo leh beero);2. Axdiga (aan-beero);3. Dhul-beereed dabiici ah
SIstaraatiijiyad isku mid ah: Isku xigxiga amplicon dhererka buuxa (ITS)
PRimers: ITS1F-ITS4 (oo lagu beegsanayo dhammaan gobolka ITS ~ 630 bp)
Sequencing platform: PacBio RS II
Falanqaynta Biyoinformatic
Natiijooyinka
OCelcelis ahaan 357 OTUs ayaa la aqoonsaday goobtiiba iyo wadarta 837 OTU ee dhammaan 60ka goobood.Kala duwanaanshaha alfa ee bulshooyinka fangas xididka ma muujinin farqi weyn oo u dhexeeya saddexda hab ee beeralayda.Si kastaba ha ahaatee, saddex kooxood oo kala duwan ayaa lagu sameeyay falanqaynta kala duwanaanshiyaha beta, taas oo muujinaysa saamaynta xooggan ee nidaamka beeralayda ee xididdada dhismeedka bulshada fangas.
Jaantus 1. Kala duwanaanta Alpha (Shannon index iyo halabuurka bulshada) iyo falanqaynta kala duwanaanta beta (falanqaynta canonical ee isku-duwayaasha aasaasiga ah) ee bulshooyinka fangas xididka
Ten keystone taxa ayaa lagu qeexay iyadoo lagu salaynayo shabakada guud ee bulshooyinka fangas ee sadexda hab ee beeralayda: 10ka qanjidh ee ugu sareeya ee leh darajada ugu saraysa, udub dhexaadka ugu sareeya iyo dhexda hoose ee dhexe ayaa la doortay.Toddoba ka mid ah waxay ka tirsan yihiin amarrada mycorrhizal.
Jaantuska 2. Isku-xidhka guud ee bulshooyinka fangas xididka ee saddexda hab-beeraeed
FHubaynta-nidaamka shabakadaha gaarka ah waxay muujiyeen isku xirnaan aad u sareysa oo shabakada organic ah oo leh laba jeer geeso badan iyo noodhadh isku xiran oo ka badan kuwa aan-ilaa lahayn iyo shabakada caadiga ah.Intaa waxa dheer, shabakada beeralayda dabiiciga ah waxa ay haysteen wax badan oo muhiim ah oo taxa (dheeman) marka la barbar dhigo inta kale, taas oo taageertay kakanaanta iyo isku xidhka
Jaantuska 3. Shabakadaha fangas ee xididka gaar ah ee nidaamka beeraha
Axiriir xun oo xoog leh oo u dhexeeya xoojinta beeraha iyo isku xirka shabakada fangas xididka ayaa la arkay.Falanqaynta kaynta random ayaa shaaca ka qaaday darawalada ugu muhiimsan ee taxa furaha: fosfooraska ciidda, cufnaanta bulk, pH iyo gumeysiga mycorrhizal.
Jaantuska 4. Xoojinta beeraha iyo isku xidhka shabakada ee saddexda hab-beeraeed (A iyo B);Falanqaynta Kaymaha Random (C) iyo Xiriirka ka dhexeeya xoojinta beeraha iyo gumeysiga AMF (D)
Farsamada
Isku xigxiga amplicon dherer buuxa
As "Taxanaha Jiilka Saddexaad" ee soo gelaya masraxa, xaddidaadda gobollada la beegsaday iyo dhibaatooyinka golaha de novo waa laga gudbay.Baasifiga Bioscience (PacBio) waxay si guul leh u kordhisay akhrinta taxanaha tobanaan kilobases, taas oo noo ogolaanaysa inaan helno akhrinta dhererka buuxa ee 16s rRNA (1,000 bp-1,500 bp) ee bakteeriyada ama 18S rRNA (1,500 bp-2,000 bp) iyo ITS gobollada (400 bp-900 bp) ee eukaryotics.Aragtida ballaaran ee beerta hidde-sidaha ayaa si weyn u xoojisay xallinta sharraxaadda noocyada iyo hiddo-wadaha shaqeynaya.Arrinka muddada dheer ka welwelsanaa ee ku saabsan saxnaanta saldhigga waxaa xalliyey is- saxista PacBio CCS, taasoo soo saarta HIFI akhrinta in ka badan 99% saxsanaanta.
Waxqabadka qoraalka OTU
Tfaa'iidooyinka akhrinta dheer iyo kuwa sare labadaba, saxnaanta tifaftirka si weyn ayaa loo kordhin karaa waxaana lagu gaari karaa xallinta "heerka noocyada" ee aqoonsiga microbial.
Tixraac
Banerjee, Samiran, iyo al."Xoojinta beeruhu waxay yaraynaysaa kakanaanta shabakadaha microbial iyo tirada badan ee canshuurta furaha ee xididdada."Wargeyska ISME (2019).
Farsamada iyo Waxyaabaha ugu muhiimsan ujeedadeedu tahay in la wadaago adeeggii ugu dambeeyay ee lagu guulaystay ee tignoolajiyada kala duwan ee isku xigxiga-sare ee saaxadda cilmi-baarista ee kala duwan iyo sidoo kale fikrado cajiib ah oo ku saabsan naqshadaynta tijaabada iyo macdanta xogta.
Waqtiga boostada: Jan-08-2022