HORUMARINTA GENOME
genetics dabeecadda
Isugeynta genome-ga oo tayo sare leh ayaa muujisa sifooyinka genomic galley iyo hidde-sidayaasha muhiimka ah ee beeraha
PacBio |Illumina |Bionano Optical Map |Hi-C Genome Assembly |Khariidadda Hidaha |Xaaqid Xul Ah |RNA-Seq |ISO-seq |SLAF-seq
Biomarker Technologies waxay bixisay taageero farsamo oo ku saabsan taxanaha Pacbio, taxanaha Hi-C iyo falanqaynta xogta daraasaddan.
Tilmaamaha
1.Qormosoomkii ugu horeeyay ee tayada sare leh ee Rye genome ayaa la helay, kaas oo leh hal cabbir koromosoom ah oo ka weyn 1 Gb.
2.Marka la barbardhigo Tu, Aet iyo Hv genome, dhacdooyin gaar ah oo dhawaanahan LTR-RT ah ayaa lagu arkay genome-ka Rye, kaas oo mas'uul ka ahaa fidinta cabbirka genome-ga galley.
3. Farqiga u dhexeeya sarreenka galley iyo diploid wuxuu dhacay ka dib markii la kala soocay shaciirka iyo sarreenka, iyadoo waqtiyada kala duwanaanshaha labada dhacdo ay ahaayeen qiyaastii 9.6 iyo 15 MYA.
Fosforyaalka hiddo-wadaha FT ayaa laga yaabaa inay xakameeyaan sifada cinwaanka hore ee galley.
4. Falanqaynta xaaqidda xulashada waxay muujinaysaa ku lug lahaanshaha suurtogalka ah ee ScID1 ee xeerinta taariikhda ciwaanka iyo xulashada suurtogalka ah ee xulashada guriga ee galley
Asalkii hore
Asalkii hore
Rye waa cunto qiimo leh iyo dalagga calafka, kheyraadka hidde-sidaha muhiimka u ah hagaajinta sarreenka iyo triticale, iyo walxo lagama maarmaan u ah daraasado wax ku ool ah oo isbarbardhigga genomics ee cawska.galley Weining, oo ah ubax hore oo kala duwan oo lagu beeray Shiinaha, ayaa ah mid aad u wanaagsan sababtoo ah caabbinta ballaaran ee caarada budada ah iyo miridhkuba.Si loo fahmo aasaaska hidda-socodka iyo molecular ee sifooyinka caanka ah ee galley iyo si kor loogu qaado cilmi-baarista genomic iyo taranta ee galley iyo dalagyada la xiriira, waxaan halkan taxnay oo falanqeynay genome of Weining galley.
Guulihii
Rye Genome
Genome-ka Rye waxaa lagu dhisay iyada oo la isku darayo akhrinta PacBio SMRT, akhrinta gaaban ee Illumina, iyo sidoo kale kuwa laga soo qaaday qabsashada chromatin conformation (Hi-C), khariidaynta hiddaha, iyo falanqaynta BioNano.Xirmooyinka la isku keenay (7.74 Gb) waxay ku xisaabtameen 98.47% qiyaasta genome-ga lagu qiyaasay (7.86 Gb), iyadoo 93.67% contigs (7.25 Gb) loo qoondeeyay todoba koromosoom.Curiyayaasha soo noqnoqda waxay ka kooban yihiin 90.31% genome-ga la soo ururiyey.
Rye Genome
Khariidadda isku xirka hidde-sideyaasha (WJ) ayaa la sameeyay iyadoo la adeegsanayo 295 F2 dhir ah oo laga soo qaatay isgoysyada labada dhul galley (Weining × Jingzhou)
Khariidadda xiriirka Hi-C ee toddobada koromosoomyada Weining Rye ee la isku keenay (1R - 7R)
Isbarbardhigga u dhexeeya toddobada koromosoom ee la isku keenay ee Weining rye iyo toddobada kooxood ee isku xidhka galley ee la sameeyay iyadoo la adeegsanayo Lo7 x Lo255 RIL
Qiimaha LTR Assembly Index (LAI) ee genome-ka Rye waxaa lagu ogaadey inuu yahay 18.42 iyo 1,393 (96.74%) ee 1,440 ee aadka loo ilaaliyo ee BUSCO ayaa la aqoonsaday. iyo gobollada genic.Wadar dhan 86,991 hiddo-sidaha borotiinka-koodka, oo ay ku jiraan 45,596-kalsooni sare (HC) hidde-sidayaasha iyo 41,395 hidde-sideyaasha kalsoonida-hoose (LC) ayaa la saadaaliyay.
2. Falanqaynta TE-yada
Falanqaynta TE-yada.Wadar dhan 6.99 Gb, oo ka dhigan 90.31% shirka Weining, ayaa loo qeexay TE, oo ay ku jiraan 2,671,941 walxood oo ay leeyihiin 537 qoys.Maaddada TE-da waxay si cad uga sarraysay tii hore loo soo sheegay Ta (84.70%), Tu (81.42%), Aet (84.40%), WEW (82.20%), ama Hv (80.80%).Terminal-ka dheer ee dib-u-celinta dib-u-celinta (LTR-RTs), oo ay ku jiraan Gypsy, Copia iyo walxaha RT aan la kala saarin, ayaa ahaa TE-yada ugu sarreeya, oo 1 qabsaday 84.49% ee nuxurka TE iyo 76.29% ee genome la isku daray ee Weining;Qaadista DNA-da CACTA waxay ahaayeen TE-yada labaad ee ugu badan, oo ka kooban 11.68% ee nuxurka TE iyo 10.55% ee genome-ga la isku keenay ee Weining.
Falanqaynta walxaha transposon ee galley
Xayndaabka galleydu waxa ay lahayd qayb is barbar dhig ah oo aad u saraysa oo ah galinta dhawaantan ee LTR-RT-yada oo leh tii ugu saraysay ee kor u qaadistoodu waxa ay soo muuqatay qiyaastii 0.5 milyan sano ka hor (MYA), kaas oo ahaa kii ugu dambeeyay ee afarta nooc;Midka ugu sarreeya, wuxuu dhacay qiyaastii 1.7 MYA, wuu da' weynaa oo sidoo kale lagu arkay shaciir.Heer sare qoys, dilaacyadii ugu dambeeyay ee curiyeyaasha Copia ee Weining galley ee 0.3 MYA ayaa la helay, halka kordhinta Gypsy RTs ay si weyn u qaabeeyeen qaabka qaybinta laba-geesoodka ah ee dhaqdhaqaaqa dilaaca LTR-RT.
3. Baaritaanka korriinka genome-ga ee galley iyo chromosome syntenies
Farqiga u dhexeeya galleyda iyo sarreenka diploid ayaa dhacay ka dib markii laga soocay shaciir ka sarreenka, iyada oo waqtiyada kala duwanaanshaha labada dhacdo ay ahaayeen qiyaastii 9.6 iyo 15 MYA, siday u kala horreeyaan.1R, 2R, 3R waxay si buuxda u wada siman yihiin kooxaha 1, 2 iyo 3 koromosoomyada sarreenka, siday u kala horreeyaan.4R, 5R, 6R, 7R ayaa la helay inay jiraan fiyuusyo baaxad weyn iyo qaybo.
4. Falanqaynta isku-duubnida hiddo-wadaha iyo saamaynta ay ku leeyihiin hidda-wadaha biosynthesis ee istaarijka
Waxaa xusid mudan, tirooyinka hiddo-wadaha la isku koobay (TDGs) iyo hiddo-wadaha u dhow-dhow (PDGs) ee Weining galley labaduba waxay ka badnaayeen kuwa laga helay Tu, Aet, Hv, Bd iyo Os.Hidde-sidayaasha la soo koobay (TrDGs) ayaa sidoo kale ka badnaa kuwa si gaar ah loogu helay Tu iyo Aet.Balaadhinta genome-ka Rye waxaa weheliya tiro badan oo isku-duubnida hiddo-wadaha.Korodhka TE ee dilaaca galleyda ayaa laga yaabaa inay keentay tiro sare oo TrDGs ah.
Falanqaynta korriinka iyo koromosoomyada synteny ee genome-ga galley
Falanqaynta nuqul ka mid ah hiddo-wadaha galleyda iyo saamaynta ay ku leeyihiin noocyada kala duwan ee hidda-sidaha biosynthesis ee istaarijka (SBRGs)
5. Kala qaybinta borotiinka kaydinta abuurka galley (SSP) ee loci
Afar koromosoomyada loci (Sec-1 ilaa Sec-4) oo tilmaamaya SSPs galley ayaa lagu aqoonsaday 1R ama 2R.Hidde-sidayaasha α-gliadin waxa ay dhawaan uun ku kobceen sarreenka iyo noocyada xidhiidhka dhow ka dib kala duwanaanshaha sarreenka ee galley.
6. Baadhitaanka factor transcription (TF) iyo hidde iska caabinta cudurada
Falanqaynta galley secalin loci
Weining galley wuxuu lahaa tiro badan oo iska caabin cudur ah oo la xiriira (DRA) hiddo-wadaha (1,989, Xogta Dheeraadka 3) marka loo eego Tu (1,621), Aet (1,758), Hv (1,508), Bd (1,178), Os (1,575), iyo A (1,836) ), B (1,728) iyo D (1,888) subgenomyyada sarreenka caadiga ah.
7. Baadhitaanka astaamaha muujinta hidda-wadaha ee la xidhiidha sifada ciwaanka hore
Laba hidde-side FT oo leh muujin heer sare ah oo hoos yimaada xaaladaha maalinta dheer, ScFT1 iyo ScFT2, ayaa lagu sharraxay isu imaatinka genome ee Weining.Laba haraaga amino acid ee ScFT2 (S76 iyo T132) fosforyaalka ayaa la helay xiriir la leh hoos u dhigista xakamaynta wakhtiga
Tilmaamaha muujinta korriinka iyo hidda-wadaha ee la xidhiidha sifada cinwaanka hore ee Weining galley
8. Macdanta gobollada koromosoomyada iyo meelaha laga yaabo inay ku lug yeeshaan guriyeynta galley
Wadar dhan 123,647 SNPs ayaa loo isticmaalay in lagu sameeyo falanqayn xaaqin ah oo u dhexeeya galley la beeray iyo S.vavilovii.11 calaamado xaaqid la xushay oo lagu aqoonsaday index dhimis (DRI), index hagaajinta (FST) iyo habka XP-CLR.ScID1 waxaa la helay ku lug lahaanshaha suurtagalka ah ee sharciyeynta taariikhda cinwaanka.
Aqoonsiga iyo falanqaynta gobollada koromosoomyada iyo meelaha suurtagalka ah ee la xidhiidha guriyaynta galley
Tixraac
Li GW iyo al.Isugeynta genome-ga oo tayo sare leh ayaa muujisa sifooyinka genomic galley iyo hidde-sidayaasha muhiimka ah ee beeraha.Hiddaha Dabeecada (2021)
Warar iyo Qodobo Muhiim ah ujeedadeedu tahay in la wadaago kiisaskii ugu dambeeyay ee lagu guulaystey Teknolojiyadda Biomarker, qabashada guulaha sayniska ee cusub iyo sidoo kale farsamooyinka caanka ah ee lagu dabaqay daraasadda.
Waqtiga boostada: Jan-05-2022