Alaabta

Fungal Genome

Tiknoolajiyada Biomarker waxay bixiyaan sahan genome, genome ganaax ah iyo genome-dhameystiran genome ee fangas iyadoo ku xiran hadafka cilmi baarista gaarka ah.Taxanaha genome-ga, isu-ururinta iyo sharraxaadda shaqada waxa lagu gaadhi karaa iyadoo la isku darayo taxanaha jiilka xiga + taxanaha jiilka saddexaad si loo gaadho isu-ururinta genome-ga heerka sare ah.Tignoolajiyada Hi-C sidoo kale waxaa loo adeegsan karaa si loo fududeeyo isu-ururinta genome-ga heerka koromosoomyada.

Platform:Talaabada PacBio II

Nanopore PromethION P48

Illumina NovaSeq Platform

Nala soo xiriir

Faahfaahinta Adeegga

Natiijooyinka Demo

Faa'iidooyinka Adeegga

● Xeelado isku xigxig oo kala duwan oo loo heli karo ujeedooyin cilmi baaris oo kala duwan

● Khibrad sare u leh isu imaatinka genome fungi oo leh in ka badan 10,000 oo genome dhammaystiran oo la isugu keenay.

● Kooxda taageerada farsamada iibka ka dib ee xirfadlayaasha ah oo buuxinaya baahiyo cilmi-baaris oo gaar ah.

Tilmaamaha Adeegga

Adeegga	Istaraatiijiyada Taxanaha	Tayada la damaanad qaaday	Waqtiga soo-jeedinta la qiyaasay
Fungal khariidad fiican	Illumina 50X+ Nanopore 100X	Ku socota N50≥2 Mb	35 maalmood oo shaqo
Fungal khariidad fiican	PacBio HiFi 30X	Ku socota N50≥2 Mb	35 maalmood oo shaqo
Maabka fangas-ku-buuxsan yahay	Illumina 50X+ Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Qiyaasta ku xidhidhiyaha Chromosome>90%	45 maalmood oo shaqo

Falanqaynta bioinformatics

● Xakamaynta tayada xogta ceeriin

● Kulanka genome

● Falanqaynta qaybaha genome

● Sharaxaada shaqada hidda-wadaha

● Falanqaynta genomic isbarbardhigga

Tusaalaha Shuruudaha iyo Bixinta

Tusaalaha Shuruudaha:

WaayoSoosaarida DNA:

Tusaalaha Nooca	Qadarka	Xoog-saarid	daahirnimo
Soosaarida DNA	1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Shaybaarada unugyada:

Tusaalaha nooca

daawaynta muunad ee lagu taliyay

Tusaalaha kaydinta iyo rarista

Fungus Unicellular

Ku ilaali khamiirka hoosta mikroskoob oo ku soo ururi wejigooda jibbaarada

U wareeji dhaqanka (oo ka kooban qiyaastii. 3-4.5e9 unug) una wareeji 1.5 ama 2 ml eppendorf.(Barafka ku hay)

Centrifuge tuubada 1 daqiiqo at 14000 g si aad u ururiso bakteeriyada oo aad si taxadar leh uga saartid dusha sare

Xidh tuubada oo ku qabooji bakteeriyada ku jirta nitrogen dareeraha ah ugu yaraan 1-3 saacadood.Ku kaydi tuubada qaboojiyaha -80 ℃.

Ku qabooji shaybaarada ku jira nitrogen dareeraha ah 3-4 saacadood kuna kaydi nitrogen dareeraha ah ama -80 digrii ilaa boos sii hayn muddo dheer ah.Tusaalaha rarida baraf qallalan ayaa loo baahan yahay.

Fungus macro

Nudaha wajiga si firfircoon u koraya ayaa lagula talinayaa.

Ku raaci nudaha biyo aan lahayn endotoxin, ka dibna 70% ethonal.

Ku kaydi muunada tuubooyinka cryo-tubes.