Hiddaha Kobcinta
Faa'iidooyinka Adeegga
Takagi et al.,Joornaalka dhirta, 2013
● Qiyaasta kala duwanaanshaha noocyada kala duwanaanshaha wakhtiga iyo xawaaraha ku salaysan kala duwanaanta heerka nucleotide iyo amino acids
● Daaha ka qaadida xidhiidhka phylogenetic ee la isku halayn karo ee u dhexeeya noocyada leh saamaynta la yareeyey ee korriinka is-daba-joogga ah iyo kobaca is barbar socda
● Dhisidda xidhiidhka ka dhexeeya isbeddellada hidde-sidaha iyo noocyada phenotypes si loo ogaado hidde-sidaha la xidhiidha
● Qiyaasta kala duwanaanshaha hidde-sideyaasha, taas oo ka tarjumaysa awoodda korriinka ee noocyada
● Wakhtiga ka-soo-noqoshada oo degdeg ah
● Khibrad ballaadhan: BMK waxa ay ururisay waayo-aragnimo ballaadhan oo xagga dadweynaha iyo mashaariicda horumarka la xidhiidha in ka badan 12 sano, oo daboolaysa boqollaal nooc, iwm. waxayna ka qayb qaadatay in ka badan 80 mashruuc oo heer sare ah oo lagu daabacay Isgaarsiinta Dabiiciga ah, Dhirta Molecular, Journal Biotechnology Journal, iwm.
Tilmaamaha Adeegga
Qalabka:
Caadiyan, ugu yaraan saddex dad-hoosaadyo (tusaale nooc-hoosaad ama nooc) ayaa lagula talinayaa.Dad-hoosaad kastaa waa inuu ka kooban yahay wax aan ka yarayn 10 qof (Dhirta 15, waa la dhimi karaa noocyada dhifka ah).
Istaraatiijiyada isku xigxiga:
* WGS waxaa loo shaqaaleysiin karaa noocyada leh hiddo-wadaha tixraaca tayada sare leh, halka SLAF-Seq lagu dabaqi karo noocyada midkood ama aan lahayn genome tixraaceed, ama genome tixraac tayo liita.
| Lagu dabaqi karo cabbirka genome-ga | WGS | SLAF-Tags (×10,000) |
| ≤ 500 Mb | 10×/ shaqsi | WGS ayaa aad loogu talinayaa |
| 500 Mb - 1 Gb | 10 | |
| 1 Gb - 2 Gb | 20 | |
| ≥2 Gb | 30 |
Falanqaynta bioinformatics
● Falanqaynta korriinka
● Xaaqid xul ah
● Qulqulka hidda-socodka
● Taariikhda tirada dadka
● Wakhtiga kala duwanaanshaha
Tusaalaha Shuruudaha iyo Bixinta
Tusaalaha Shuruudaha:
| Noocyada | Nudaha | WGS-NGS | SLAF |
| Xoolaha
| Nudaha indhaha |
0.5-1g
|
0.5g
|
| Nudaha murqaha | |||
| Dhiiga naasleyda | 1.5ml
| 1.5ml
| |
| Dhiiga digaaga/kalluunka | |||
| Dhirta
| Caleen Cusub | 1 ~ 2g | 0.5-1g |
| Dufan/jirrid | |||
| Xidid/Shiil | |||
| Unugyada | Unug dhaqan |
| gDNA | Xoog-saarid | Qadarka (ug) | OD260/OD280 |
| SLAF | ≥35 | ≥1.6 | 1.6-2.5 |
| WGS-NGS | ≥1 | ≥0.1 | - |
Socodka Shaqada Adeegga
Naqshadaynta tijaabada
Tusaalaha keenista
Dhismaha maktabadda
Isku xigxiga
Falanqaynta xogta
Adeegyada iibka kadib
*Natiijooyinka demo ee halkan lagu muujiyay dhamaantood waxay ka yimaadeen genome-yada lagu daabacay BMKGENE
1. Falanqaynta Evolution waxa ay ka kooban tahay dhismaha geedka phylogenetic, qaab-dhismeedka dadweynaha iyo PCA-ga oo ku salaysan kala duwanaanshiyaha hiddaha.
Geedka Phylogenetic wuxuu u taagan yahay cilaaqaadka taxonomic iyo kobcinta ee ka dhexeeya noocyada awoowayaasha wadaaga ah.
PCA waxa ay rabta in ay sawirto isku soo dhawaanshaha ka dhexeeya bulshooyin hoosaadyada.
Qaab dhismeedka dadku wuxuu muujinayaa joogitaanka dad-hoosaadyo hidde ahaan kala duwan marka loo eego inta jeer ee allele.
Chen, iyo.al.,PNAS, 2020
2.Xaqid la doortay
Xaaqid xulashada ah waxa loola jeedaa hab lagu doorto goob faa'iido leh iyo inta jeer ee goobaha dhexdhexaadka ah ee ku xiran la kordhiyo iyo kuwa aan la xiriirin oo la dhimo, taasoo keentay hoos u dhaca gobolka.
Ogaanshaha genome-ballaaran ee gobollada xaaqin ee xulashada waxaa lagu farsameeyaa xisaabinta tusaha hidda-socodka dadweynaha (π, Fst, Tajima's D) ee dhammaan SNP-yada gudaha daaqad simbiriirixan (100 Kb) tallaabo go'an (10 Kb).
Kala duwanaanshaha Nucleotide (π)
Tajima ee D
Tilmaanta hagaajinta (Fst)
Wu, iyo.al.,Warshada Molecular, 2018
3. Socodka Hiddaha
Wu, iyo.al.,Warshada Molecular, 2018
4.Taariikhda tirakoobka
Zhang, iyo.al.,Nature Ecology&Evolution, 2021
5.Waqtiga kala duwanaanshaha
Zhang, iyo.al.,Nature Ecology&Evolution, 2021
Kiiska BMK
Khariidadda kala duwanaanshiyaha genomic waxay siinaysaa fikrado ku saabsan saldhigga hidde-sidaha ee xulashada Kaabashka Shiinaha (Brassica rapa ssp. Pekinensis)
La daabacay: Warshada Molecular, 2018
Istaraatiijiyada isku xigxiga:
Ku-xigta: qoto dheer ee isku xigxiga: 10×
Natiijooyinka muhiimka ah
Daraasaddan, 194 kaabash Shiineys ah ayaa loo habeeyey dib-u-isku-dabasho leh celcelis ahaan qoto dheer oo ah 10 ×, taas oo keentay 1,208,499 SNPs iyo 416,070 InDels.Falanqaynta phylogenetic ee 194-kan sadar waxay muujisay in khadadkan loo qaybin karo saddex nooc oo deegaanka ah, gu', xagaaga iyo dayrta.Intaa waxaa dheer, qaab-dhismeedka dadweynaha iyo falanqaynta PCA-ga ayaa tilmaamay in kaabashka Shiinaha ee gu'ga uu ka yimid kaabajka dayrta ee Shandong, Shiinaha.Kuwaas waxaa markii dambe loo soo bandhigay Kuuriya iyo Japan, oo ay ka gudbeen khadadka maxalliga ah iyo qaar ka mid ah noocyo dambe oo iyaga ka mid ah ayaa dib loogu soo celiyay Shiinaha ugu dambeyntiina waxay noqdeen kaabajka Shiinaha ee Guga.
Baadhitaanka genome-ballaaran ee kaabajka Shiinaha ee guga iyo kaabajka dayrta ee xulashada ayaa shaaca ka qaaday 23 genomic loci kuwaas oo soo maray xulasho xooggan, oo laba ka mid ah ay ku dhejiyeen gobolka xakamaynta-waqtiga ku salaysan QTL-maping.Labadan gobol ayaa la ogaaday inay ku jiraan hidde-sideyaasha muhiimka ah ee nidaamiya ubaxa, BrVIN3.1 iyo BrFLC1.Labadan hiddo-wade ayaa sidoo kale la xaqiijiyay inay ku lug leeyihiin waqti-kordhinta daraasadda transcriptome iyo tijaabooyinka transgenic.
 Falanqaynta qaab-dhismeedka dadweynaha ee kaabajka Shiinaha |  Macluumaadka hidda-socodka ee xulashada kaabajka Shiinaha |
Tongbing, iyo al."Khariidadda Kala duwanaanshiyaha Genomic wuxuu bixiyaa aragtida asalka hidda-socodka ee Kaabashka Shiinaha (Brassica rapa ssp.pekinensis) doorashada."Dhirta molecular,11 (2018): 1360-1376.
