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Epigenetics

  • Chromatin Immunoprecipitation Sequencing (ChIP-seq)

    Chromatin Tallaalka Tallaalka (ChiP-seq)

    ChIP-Seq waxay bixisaa sifaynta genom-ballaaran ee bartilmaameedyada DNA-da ee wax ka beddelka histone, qodobbada qoraalka, iyo borotiinada kale ee DNA-da la xidhiidha.Waxay isku daraysaa xulashada chromatin immuno-precipitation (ChIP) si ay u soo kabsato dhismooyin borotiin-DNA gaar ah, oo leh awoodda isku xigxiga jiilka xiga (NGS) ee isku xigxiga-sare ee DNA-da la soo kabsaday.Intaa waxaa dheer, sababtoo ah iskudhafka borotiinka-DNA waxaa laga soo ceshay unugyada nool, goobaha isku-xidhka ah waxaa lagu barbar dhigi karaa noocyo kala duwan oo unugyo ah iyo unugyo, ama xaalado kala duwan.Codsiyada waxay u dhexeeyaan nidaaminta qoraalka ilaa dariiqyada korriinka ilaa hababka cudurada iyo wixii ka baxsan.

    Platform: Illumina NovaSeq 6000

  • Whole genome bisulfite sequencing

    Isku xigxiga genome bisulfite oo dhan

    Methylation DNA ee booska shanaad ee cytosine (5-mC) ayaa saameyn aasaasi ah ku leh muujinta hiddaha iyo dhaqdhaqaaqa gacanta.Qaababka methylation-ka aan caadiga ahayn ayaa lala xiriiriyay xaalado iyo cuduro dhowr ah, sida kansarka.WGBS waxay noqotay halbeegga dahabiga ah ee lagu baranayo methylation-ka-ballaaran ee genome-ga ee xallinta hal saldhig.

    Platform: Illumina NovaSeq6000

  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

    Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo

    ATAC-seq waa hab isku xigxig heersare ah oo loogu talagalay falanqaynta gelitaanka chromatin ee genom-ballaaran, taas oo muhiim u ah xakamaynta epigenetic-ka caalamiga ah ee muujinta hiddaha.Adabiyeyaasha isku xigxiga ayaa la geliyo gobollada chromatin ee furan iyadoo loo marayo transposase firfircoon oo Tn5 ah.Kordhinta PCR ka dib, maktabad taxane ah ayaa la dhisay.Dhammaan gobollada chromatin-ka furan waxaa lagu heli karaa xaalad gaar ah oo waqti-space ah, oo aan ku xaddidnayn oo keliya goobaha lagu xirayo qodobka qoraalka, ama gobol gaar ah oo histone wax laga beddelay.

  • Reduced Representation Bisulfite Sequencing (RRBS)

    Taxanaha Bisulfite ee la dhimay (RRBS)

    Cilmi-baarista methylation DNA waxay had iyo jeer ahayd mawduuc kulul oo ku saabsan cilmi-baarista cudurrada, waxayna si dhow ula xiriirtaa muujinta hidda-wadaha iyo sifooyinka pheno-nooca.RRBS waa hab sax ah, hufan oo dhaqaale oo loogu talagalay cilmi-baarista methylation DNA.Kobcinta dhiirrigeliyeyaasha iyo gobollada jasiiradaha CpG iyadoo la kala gooyo enzymatic (Msp I), oo ay weheliso isku xigxiga Bisulfite, waxay bixisaa ogaanshaha methylation DNA xallin sare.

    Platform: Illumina NovaSeq 6000

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