Alaabta

Bakteeriyada Genome oo Dhamaystiran

Bakteeriyada Dhameystiran Genome Sawirka

Teknolojiyadda Biomarker waxay bixisaa adeeg isku xigxig ah oo lagu dhisayo genome-ka bakteeriyada oo dhammaystiran oo aan lahayn farqi eber ah.Shaqada ugu muhiimsan ee bakteeriyadu waxay dhamaystiraysaa dhismaha genome-ga waxaa ka mid ah isku xigxiga jiilka saddexaad, isu-ururinta, sharraxaadda shaqeynta iyo falanqaynta bioinformatic-ka sare ee buuxinaysa yoolalka cilmi-baarista gaarka ah.Bakteeriyada genome-ka ee genome-ka ayaa awood u siinaya muujinta hababka aasaasiga ah ee ka hooseeya hababka bayoolojiga, taas oo sidoo kale siin karta tixraac qiimo leh cilmi-baarisyada genomic ee noocyada sare ee eukaryotic.

Platform:Nanopore PromethION P48 + Illumina NovaSeq Platform

Talaabada PacBio II

Nala soo xiriir

Faahfaahinta Adeegga

Natiijooyinka Demo

Faa'iidooyinka Adeegga

● Xeelado isku xigxig oo kala duwan oo loo heli karo ujeedooyin cilmi baaris oo kala duwan

● Bakteeriyadu waxay dhammaystiran tahay genome oo leh 0 Gap dammaanad ah.

● Khibrad sare u leh bakteeriyada genome-ga oo la isku keenay in ka badan 10,000 oo genom oo dhamaystiran.

● Kooxda taageerada farsamada iibka ka dib ee xirfadlayaasha ah oo buuxinaya baahiyo cilmi-baaris oo gaar ah.

Tilmaamaha Adeegga

Isku xigxigaXeeladda

Maktabadda

Tayada la damaanad qaaday

Waqtiga soo-jeedinta la qiyaasay

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Farqiga

30 Maalmood

PacBio HiFi 30X

PacBio 10K

Falanqaynta bioinformatics

● Xakamaynta tayada xogta ceeriin

● Kulanka genome

● Falanqaynta qaybaha genome

● Sharaxaada shaqada hidda-wadaha

● Falanqaynta genomic isbarbardhigga

Tusaalaha Shuruudaha iyo Bixinta

Tusaalaha Shuruudaha:

WaayoSoosaarida DNA:

Tusaalaha Nooca	Qadarka	Xoog-saarid	daahirnimo
Soosaarida DNA	2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Shaybaarada unugyada:

Tusaalaha nooca

daawaynta muunad ee lagu taliyay

Tusaalaha kaydinta iyo rarista

Bakteeriyada

Bakteeriyada ku fiiri mikroskoob ka dibna ku ururi bakteeriyada wejigeeda jibbaarada

U wareeji dhaqanka bakteeriyada (oo ka kooban qiyaastii 3-4.5e9 unug) una wareeji 1.5 ama 2 ml eppendorf.(Barafka ku hay)

Centrifuge tuubada 1 daqiiqo at 14000 g si aad u ururiso bakteeriyada oo aad si taxadar leh uga saartid dusha sare

Xidh tuubada oo ku qabooji bakteeriyada ku jirta nitrogen dareeraha ah ugu yaraan 30 daqiiqo.Ku kaydi tuubada qaboojiyaha -80 ℃.

Ku qabooji shaybaarada ku jira nitrogen dareeraha ah 3-4 saacadood kuna kaydi nitrogen dareeraha ah ama -80 digrii ilaa boos sii hayn muddo dheer ah.Tusaalaha rarida baraf qallalan ayaa loo baahan yahay.