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Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo

Qiimaynta Chromatin-ka-soo-baxa ee la heli karo oo leh taxanayaal wax-soo-saar sare leh (ATAC-seq) Sawirka
  • Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo
  • Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo
  • Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo
  • Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo
  • Qiimaynta chromatin-ka-wareejin-ka-hortagga leh ee la heli karo

ATAC-seq waa hab isku xigxig heersare ah oo lagu falanqeeyo gelitaanka chromatin-ka genom-ballaaran, taas oo muhiim u ah xakamaynta epigenetic-ka caalamiga ah ee muujinta hidda-wadaha.Adabiyeyaasha isku xigxiga ayaa la geliyo gobollada chromatin ee furan iyadoo loo marayo transposase firfircoon oo Tn5 ah.Kordhinta PCR ka dib, maktabad taxane ah ayaa la dhisay.Dhammaan gobollada chromatin-ka furan waxaa lagu heli karaa xaalad gaar ah oo waqti-space ah, oo aan ku xaddidnayn oo keliya goobaha lagu xirayo qodobka qoraalka, ama gobol gaar ah oo histone wax laga beddelay.


Faahfaahinta Adeegga

Bioinformatics

Natiijooyinka Demo

Faa'iidooyinka Adeegga

● Falanqaynta Dhamaystiran: dhuumaha falanqaynta xirfadlayaasha si ay u daboolaan baahiyo kala duwan oo la habeeyey;

● Khibrad Balaadhan: Boqolaal mashruuc oo si guul leh loo dhameeyay ilaa hadda, oo ay ku jiraan muunadaha xoolaha iyo dhirta.

Tilmaamaha Adeegga

Platform: Illumina NovaSeq platform

Nooca maktabadda: ATAC-seq

Soo saarista xogta lagu taliyay: ≥20M akhriyay

Shuruudaha Tusaalaha

Nooca Tusaalaha: Unugyo, unugyo nool, ama unugyo la qaboojiyey iyo wixii la mid ah

Lambarka gacanta: ≥ 5×105unugyo, lambar unug sax ah ayaa fure u ah guusha tijaabada;

Miisaanka unugyada: ≥ 200mg unug cusub;

Dhiiga: ≥ 2 ml

Socodka Shaqada Adeegga

Tusaalaha QC

Naqshadaynta tijaabada

muunad keenid

Tusaalaha keenista

Tijaabada tijaabada

Soo saarista DNA

Diyaarinta Maktabadda

Dhismaha maktabadda

Isku xigxiga

Isku xigxiga

Falanqaynta xogta

Falanqaynta xogta

Adeegyada iibka kadib

Adeegyada iibka kadib

  • Hore:
  • Xiga:

    • 表观-02

    1.Kariidada kulaylka ee ATAC waxay akhridaa qaybinta TSS iyo gobollada ku dhow (± 3 kb)

    3 (1)2. Qaybinta gobolka chromatin furan ee qaybaha genome ee kala duwan

    4 (1)

    3. Kala duwanaanshaha ugu sarreeya ee u dhexeeya kooxaha

    5 (1)

    qaado xigasho

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